11 results on '"Haycraft, Courtney J."'
Search Results
2. Generation of a New Mouse Model Harboring the Polycystin-2 Loss-of-Function D511V Patient Variant
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Outeda, Patricia, Haycraft, Courtney J., Basquin, Denis, Tran, Pamela V., Parnell, Stephen C., DeCaen, Paul G., Wallace, Darren P., Yoder, Bradley K., and Watnick, Terry J.
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- 2023
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3. A kidney resident macrophage subset is a candidate biomarker for renal cystic disease in preclinical models
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Li, Zhang, Zimmerman, Kurt A., Cherakara, Sreelakshmi, Chumley, Phillip H., Collawn, James F., Wang, Jun, Haycraft, Courtney J., Song, Cheng J., Chacana, Teresa, Andersen, Reagan S., Croyle, Mandy J., Aloria, Ernald J., Hombal, Raksha P., Thomas, Isis N., Chweih, Hanan, Simanyi, Kristin L., George, James F., Parant, John M., Mrug, Michal, and Yoder, Bradley K.
- Abstract
Although renal macrophages have been shown to contribute to cyst development in polycystic kidney disease (PKD) animal models, it remains unclear whether there is a specific macrophage subpopulation involved. Here, we analyzed changes in macrophage populations during renal maturation in association with cystogenesis rates in conditional Pkd2 mutant mice. We observed that CD206+ resident macrophages were minimal in a normal adult kidney but accumulated in cystic areas in adult-induced Pkd2 mutants. Using Cx3cr1 null mice, we reduced macrophage number, including CD206+ macrophages, and showed that this significantly reduced cyst severity in adult-induced Pkd2 mutant kidneys. We also found that the number of CD206+ resident macrophage-like cells increased in kidneys and in the urine from autosomal-dominant PKD (ADPKD) patients relative to the rate of renal functional decline. These data indicate a direct correlation between CD206+ resident macrophages and cyst formation, and reveal that the CD206+ resident macrophages in urine could serve as a biomarker for renal cystic disease activity in preclinical models and ADPKD patients. This article has an associated First Person interview with the first author of the paper.
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- 2023
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4. Chapter 11 Cilia Involvement in Patterning and Maintenance of the Skeleton.
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Haycraft, Courtney J. and Serra, Rosa
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Abstract: Although the expression of cilia on chondrocytes was described over 40 years ago, the importance of this organelle in skeletal development and maintenance has only recently been recognized. Primary cilia are found on most mammalian cells and have been shown to play a role in chemosensation and mechanosensation. A growing number of human pleiotropic syndromes have been shown to be associated with ciliary or basal body dysfunction. Skeletal phenotypes, including alterations in limb patterning, endochondral bone formation, craniofacial development, and dentition, have been described in several of these syndromes. Additional insights into the potential roles and mechanisms of cilia action in the mammalian skeleton have been provided by research in model organisms including mouse and zebrafish. In this article we describe what is currently known about the localization of cilia in the skeleton as well as the roles and underlying molecular mechanisms of cilia in skeletal development. [Copyright &y& Elsevier]
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- 2008
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5. Telomerase immortalization of principal cells from mouse collecting duct
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Steele, Stacy L., Wu, Yongren, Kolb, Robert J., Gooz, Monika, Haycraft, Courtney J., Keyser, Kent T., Guay-Woodford, Lisa, Yao, Hai, and Bell, P. Darwin
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Recently, the use of overexpression of telomerase reverse transcriptase (TERT) has led to the generation of immortalized human cell lines. However, this cell immortalization approach has not been reported in well-differentiated mouse cells, such as renal epithelial cells. We sought to establish and then characterize a mouse collecting duct cell line, using ectopic expression of mTERT. Isolated primary cortical collecting duct (CCD) cell lines were transduced with mouse (m)TERT, using a lentiviral vector. mTERT-negative cells did not survive blasticidin selection, whereas mTERT-immortalized cells proliferated in selection media for over 40 subpassages. mTERT messenger RNA and telomerase activity was elevated in these cells, compared with an SV40-immortalized cell line. Flow cytometry with Dolichos biflorusagglutinin was used to select the CCD principal cells, and we designated this cell line mTERT-CCD. Cells were well differentiated and exhibited morphological characteristics typically found in renal epithelial cells, such as tight junction formation, microvilli, and primary cilia. Further characterization using standard immunofluorescence revealed abundant expression of aquaporin-2 and the vasopressin type 2 receptor. mTERT-CCD cells exhibited cAMP-stimulated/benzamil-inhibited whole cell currents. Whole cell patch-clamp currents were also enhanced after a 6-day treatment with aldosterone. In conclusion, we have successfully used mTERT to immortalize mouse collecting duct cells that retain the basic in vivo phenotypic characteristics of collecting duct cells. This technique should be valuable in generating cell lines from genetically engineered mouse models.
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- 2010
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6. Evolutionarily conserved genetic interactions between nphp-4and bbs-5mutations exacerbate ciliopathy phenotypes
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Bentley-Ford, Melissa R, LaBonty, Melissa, Thomas, Holly R, Haycraft, Courtney J, Scott, Mikyla, LaFayette, Cameron, Croyle, Mandy J, Andersen, Reagan S, Parant, John M, and Yoder, Bradley K
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Primary cilia are sensory and signaling hubs with a protein composition that is distinct from the rest of the cell due to the barrier function of the transition zone (TZ) at the base of the cilium. Protein transport across the TZ is mediated in part by the BBSome, and mutations disrupting TZ and BBSome proteins cause human ciliopathy syndromes. Ciliopathies have phenotypic variability even among patients with identical genetic variants, suggesting a role for modifier loci. To identify potential ciliopathy modifiers, we performed a mutagenesis screen on nphp-4mutant Caenorhabditis elegansand uncovered a novel allele of bbs-5. Nphp-4;bbs-5double mutant worms have phenotypes not observed in either individual mutant strain. To test whether this genetic interaction is conserved, we also analyzed zebrafish and mouse mutants. While Nphp4mutant zebrafish appeared overtly normal, Bbs5mutants exhibited scoliosis. When combined, Nphp4;Bbs5double mutant zebrafish did not exhibit synergistic effects, but the lack of a phenotype in Nphp4mutants makes interpreting these data difficult. In contrast, Nphp4;Bbs5double mutant mice were not viable and there were fewer mice than expected carrying three mutant alleles. In addition, postnatal loss of Bbs5in mice using a conditional allele compromised survival when combined with an Nphp4allele. As cilia are still formed in the double mutant mice, the exacerbated phenotype is likely a consequence of disrupted ciliary signaling. Collectively, these data support an evolutionarily conserved genetic interaction between Bbs5and Nphp4alleles that may contribute to the variability in ciliopathy phenotypes.
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- 2022
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7. Caenorhabditis elegans DYF-2, an Orthologue of Human WDR19, Is a Component of the Intraflagellar Transport Machinery in Sensory Cilia
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Efimenko, Evgeni, Blacque, Oliver E., Ou, Guangshuo, Haycraft, Courtney J., Yoder, Bradley K., Scholey, Jonathan M., Leroux, Michel R., and Swoboda, Peter
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The intraflagellar transport (IFT) machinery required to build functional cilia consists of a multisubunit complex whose molecular composition, organization, and function are poorly understood. Here, we describe a novel tryptophan-aspartic acid (WD) repeat (WDR) containing IFT protein from Caenorhabditis elegans, DYF-2, that plays a critical role in maintaining the structural and functional integrity of the IFT machinery. We determined the identity of the dyf-2 gene by transgenic rescue of mutant phenotypes and by sequencing of mutant alleles. Loss of DYF-2 function selectively affects the assembly and motility of different IFT components and leads to defects in cilia structure and chemosensation in the nematode. Based on these observations, and the analysis of DYF-2 movement in a Bardet–Biedl syndrome mutant with partially disrupted IFT particles, we conclude that DYF-2 can associate with IFT particle complex B. At the same time, mutations in dyf-2 can interfere with the function of complex A components, suggesting an important role of this protein in the assembly of the IFT particle as a whole. Importantly, the mouse orthologue of DYF-2, WDR19, also localizes to cilia, pointing to an important evolutionarily conserved role for this WDR protein in cilia development and function.
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- 2006
8. Caenorhabditis elegansDYF-2, an Orthologue of Human WDR19, Is a Component of the Intraflagellar Transport Machinery in Sensory Cilia
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Efimenko, Evgeni, Blacque, Oliver E., Ou, Guangshuo, Haycraft, Courtney J., Yoder, Bradley K., Scholey, Jonathan M., Leroux, Michel R., and Swoboda, Peter
- Abstract
The intraflagellar transport (IFT) machinery required to build functional cilia consists of a multisubunit complex whose molecular composition, organization, and function are poorly understood. Here, we describe a novel tryptophan-aspartic acid (WD) repeat (WDR) containing IFT protein from Caenorhabditis elegans, DYF-2, that plays a critical role in maintaining the structural and functional integrity of the IFT machinery. We determined the identity of the dyf-2gene by transgenic rescue of mutant phenotypes and by sequencing of mutant alleles. Loss of DYF-2 function selectively affects the assembly and motility of different IFT components and leads to defects in cilia structure and chemosensation in the nematode. Based on these observations, and the analysis of DYF-2 movement in a Bardet–Biedl syndrome mutant with partially disrupted IFT particles, we conclude that DYF-2 can associate with IFT particle complex B. At the same time, mutations in dyf-2can interfere with the function of complex A components, suggesting an important role of this protein in the assembly of the IFT particle as a whole. Importantly, the mouse orthologue of DYF-2, WDR19, also localizes to cilia, pointing to an important evolutionarily conserved role for this WDR protein in cilia development and function.
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- 2006
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9. XBX-1 Encodes a Dynein Light Intermediate Chain Required for Retrograde Intraflagellar Transport and Cilia Assembly in Caenorhabditis elegans
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Schafer, Jenny C., Haycraft, Courtney J., Thomas, James H., Yoder, Bradley K., and Swoboda, Peter
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Intraflagellar transport (IFT) is a process required for flagella and cilia assembly that describes the dynein and kinesin mediated movement of particles along axonemes that consists of an A and a B complex, defects in which disrupt retrograde and anterograde transport, respectively. Herein, we describe a novel Caenorhabditis elegansgene, xbx-1,that is required for retrograde IFT and shares homology with a mammalian dynein light intermediate chain (D2LIC). xbx-1expression in ciliated sensory neurons is regulated by the transcription factor DAF-19, as demonstrated previously for genes encoding IFT complex B proteins. XBX-1 localizes to the base of the cilia and undergoes anterograde and retrograde movement along the axoneme. Disruption of xbx-1results in cilia defects and causes behavioral abnormalities observed in other cilia mutants. Analysis of cilia in xbx-1mutants reveals that they are shortened and have a bulb like structure in which IFT proteins accumulate. The role of XBX-1 in IFT was further confirmed by analyzing the effect that other IFT mutations have on XBX-1 localization and movement. In contrast to other IFT proteins, retrograde XBX-1 movement was detected in complex A mutants. Our results suggest that the DLIC protein XBX-1 functions together with the CHE-3 dynein in retrograde IFT, downstream of the complex A proteins.
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- 2003
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10. The C. elegans homolog of the murine cystic kidney disease gene Tg737 functions in a ciliogenic pathway and is disrupted in osm-5 mutant worms
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Haycraft, Courtney J., Swoboda, Peter, Taulman, Patrick D., Thomas, James H., and Yoder, Bradley K.
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Cilia and flagella are important organelles involved in diverse functions such as fluid and cell movement, sensory perception and embryonic patterning. They are devoid of protein synthesis, thus their formation and maintenance requires the movement of protein complexes from the cytoplasm into the cilium and flagellum axoneme by intraflagellar transport (IFT), a conserved process common to all ciliated or flagellated eukaryotic cells. We report that mutations in the Caenorhabditis elegans gene Y41g9a.1 are responsible for the ciliary defects in osm-5 mutant worms. This was confirmed by transgenic rescue of osm-5(p813) mutants using the wild-type Y41g9a.1 gene. osm-5 encodes a tetratricopeptide repeat (TPR)-containing protein that is the homolog of murine polaris (Tg737), a protein associated with cystic kidney disease and left-right axis patterning defects in the mouse. osm-5 is expressed in ciliated sensory neurons in C. elegans and its expression is regulated by DAF-19, an RFX-type transcription factor that governs the expression of other genes involved in cilia formation in the worm. Similar to murine polaris, the OSM-5 protein was found to concentrate at the cilium base and within the cilium axoneme as shown by an OSM-5::GFP translational fusion and immunofluorescence. Furthermore, time-lapse imaging of OSM-5::GFP fusion protein shows fluorescent particle migration within the cilia. Overall, the data support a crucial role for osm-5 in a conserved ciliogenic pathway, most likely as a component of the IFT process. Movies available on-line: http://www.biologists.com/Development/movies/dev3342.html
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- 2001
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11. Polaris, a Protein Involved in Left-Right Axis Patterning, Localizes to Basal Bodies and Cilia
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Taulman, Patrick D., Haycraft, Courtney J., Balkovetz, Daniel F., and Yoder, Bradley K.
- Abstract
Mutations in Tg737cause a wide spectrum of phenotypes, including random left-right axis specification, polycystic kidney disease, liver and pancreatic defects, hydrocephalus, and skeletal patterning abnormalities. To further assess the biological function of Tg737and its role in the mutant pathology, we identified the cell population expressing Tg737and determined the subcellular localization of its protein product called Polaris. Tg737expression is associated with cells possessing either motile or immotile cilia and sperm. Similarly, Polaris concentrated just below the apical membrane in the region of the basal bodies and within the cilia or flagellar axoneme. The data suggest that Polaris functions in a ciliogenic pathway or in cilia maintenance, a role supported by the loss of cilia on the ependymal cell layer in ventricles of Tg737orpkbrains and by the lack of node cilia inTg737Δ2-3βGalmutants.
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- 2001
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