1. Difference in the ligand affinity among redundant plant hormone receptors of rice OsCOI1a/1b/2-OsJAZs.
- Author
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Taichi Okumura, Tsumugi Kitajima, Takuya Kaji, Haruyuki Urano, Kotaro Matsumoto, Hideo Inagaki, Koji Miyamoto, Kazunori Okada, and Minoru Ueda
- Subjects
HORMONE receptors ,RICE ,PROTEIN-protein interactions ,ABIOTIC stress ,GENE expression ,JASMONATE ,PLANT hormones - Abstract
(3R, 7S)-jasmonoyl-L-isoleucine (JA-Ile) is a lipid-derived plant hormone that regulates plant responses, including biotic/abiotic stress adaptation. In the plant cells, JA-Ile is perceived by COI1-JAZ co-receptor by causing protein-protein interaction between COI1 and JAZ proteins to trigger gene expressions. In this study, we focused on Oryza sativa, a model monocot and an important crop, with 45 possible OsCOI-OsJAZ co-receptor pairs composed of three OsCOI homologs (OsCOI1a, OsCOI1b, and OsCOI2) and 15 OsJAZ homologs. We performed fluorescein anisotropy and pull-down assays to examine the affinity between JA-Ile and OsCOI1a/1b/2-OsJAZ1-15 co-receptor pairs. The results revealed a remarkable difference in the modes of ligand perception by OsCOI1a/1b and OsCOI2. Recently, the unique function of OsCOI2 in some of the JA-responses were revealed. Our current results will lead to the possible development of OsCOI2-selective synthetic ligand. [ABSTRACT FROM AUTHOR] more...
- Published
- 2023
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