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For the first time, enantiomer separation was performed using a polymeric flow-through-type chromatographic support (POROS). Bovine serum albumin (BSA) covalently bound to POROS was employed as the chiral stationary phase. Using flow-rates of up to 10 ml/min, enantiomer separation of all investigated compounds, a variety of amino acid derivatives and drugs, could be achieved within a few minutes at medium efficiencies typical for protein chiral stationary phases. It could be shown that the loss in resolution Rswith increasing velocity, u, of the mobile phase was lower than predicted by the theory for conventional supports, but not independent of the velocity, u, as expected by perfusion chromatographic theory.