19 results on '"Hong, Yifan"'
Search Results
2. Single-Cell Study Unveils Lead Lifespan in Blood Cell Populations Follows a Universal Lognormal Distribution with Individual Skewness
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Jiang, Hui, Gao, Yingying, Chen, Xiaoxiang, Wang, Boqian, Xu, Zhixiao, Li, Yiyang, Sun, Xinyi, Liu, Kun, Divsalar, Adeleh, Cheung, Edwin, Jiang, Lai, Hong, Yifan, and Ding, Xianting
- Abstract
Lead is a widespread environmental hazard that can adversely affect multiple biological functions. Blood cells are the initial targets that face lead exposure. However, a systematic assessment of lead dynamics in blood cells at single-cell resolution is still absent. Herein, C57BL/6 mice were fed with lead-contaminated food. Peripheral blood was harvested at different days. Extracted red blood cells and leukocytes were stained with 19 metal-conjugated antibodies and analyzed by mass cytometry. We quantified the time-lapse lead levels in 12 major blood cell subpopulations and established the distribution of lead heterogeneity. Our results show that the lead levels in all major blood cell subtypes follow lognormal distributions but with distinctively individual skewness. The lognormal distribution suggests a multiplicative accumulation of lead with stochastic turnover of cells, which allows us to estimate the lead lifespan of different blood cell populations by calculating the distribution skewness. These findings suggest that lead accumulation by single blood cells follows a stochastic multiplicative process.
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- 2024
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3. Multitype Perception Method for Drug-Target Interaction Prediction
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Wang, Huan, Liu, Ruigang, Wang, Baijing, Hong, Yifan, Cui, Ziwen, and Ni, Qiufen
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With the growing popularity of artificial intelligence in drug discovery, many deep-learning technologies have been used to automatically predict unknown drug-target interactions (DTIs). A unique challenge in using these technologies to predict DTI is fully exploiting the knowledge diversity across different interaction types, such as drug-drug, drug-target, drug-enzyme, drug-path, and drug-structure types. Unfortunately, existing methods tend to learn the specifical knowledge on each interaction type and they usually ignore the knowledge diversity across different interaction types. Therefore, we propose a multitype perception method (MPM) for DTI prediction by exploiting knowledge diversity across different link types. The method consists of two main components: a type perceptor and a multitype predictor. The type perceptor learns distinguished edge representations by retaining the specifical features across different interaction types; this maximizes the prediction performance for each interaction type. The multitype predictor calculates the type similarity between the type perceptor and predicted interactions, and the domain gate module is reconstructed to assign an adaptive weight to each type perceptor. Extensive experiments demonstrate that our proposed MPM outperforms the state-of-the-art methods in DTI prediction.
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- 2023
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4. Robotic versus laparoscopic liver resection in posterosuperior region: a retrospective study of consecutive cases
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Chen, Wei, Zhang, Xiaoyu, Jiang, Jincai, Ye, Yufu, Zhai, Zhenglong, Hu, Wendi, Li, Xiang, Chen, Yiwen, Chen, Yan, Hong, Yifan, Jia, Lan, Bai, Xueli, and Liang, Tingbo
- Abstract
Background: Minimally invasive liver resection of the posterosuperior region is considered a challenging procedure due to poor exposure and difficult bleeding control. A robotic approach is supposed to be advantageous in posterosuperior segmentectomy. Its benefits over laparoscopic liver resection (LLR) remain undetermined. This study compared robotic liver resection (RLR) and LLR in the posterosuperior region performed by a single surgeon. Materials and methods: We retrospectively analyzed consecutive RLR and LLR performed by a single surgeon between December 2020 and March 2022. Patient characteristics and perioperative variables were compared. A 1:1 propensity score matched (PSM) analysis was performed between both groups. Results: The analysis included 48 RLR and 57 LLR procedures in the posterosuperior region. After PSM analysis, 41 cases of both groups were retained. In pre-PSM cohort, the operative time in the RLR group was significantly shorter than in the LLR group (160 vs. 208 min, P= 0.001), especially in radical resection of malignant tumors (176 vs. 231 min, P= 0.004). The total Pringle maneuver duration was also markedly shorter (40 vs. 51 min, P= 0.047), and the estimated blood loss in the RLR group was lower (92 vs. 150 mL, P= 0.005). The postoperative hospital stay (POHS) in the RLR group was significantly shorter (5.4 vs. 7.5 days, P= 0.048). In PSM cohort, operative time in the RLR group was also significantly shorter (163 vs. 193 min, P= 0.036), and the estimated blood loss was lower (92 vs. 144 mL, P= 0.024). However, the total Pringle maneuver duration and POHS showed no significant difference. The complications were similar between two groups in both pre-PSM and PSM cohorts. Conclusion: RLR in the posterosuperior region was as safe and feasible as LLR. RLR was associated with reduced operative time and blood loss than LLR.
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- 2023
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5. Corrigendum to "The necroptosis-mediated imbalance of mitochondrial dynamics is involved in DEHP-induced toxicity to immature testes via the PGAM5–DRP1 interaction" [Environ Pollut. 346 (2024) Article 123585].
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Zhou, Xiazhu, Hong, Yifan, Chen, Jing, Yu, Yihang, Wang, Siyuan, Wei, Yuexin, Long, Chunlan, Shen, Lianju, Wu, Shengde, and Wei, Guanghui
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TESTIS ,MITOCHONDRIA - Published
- 2024
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6. The necroptosis-mediated imbalance of mitochondrial dynamics is involved in DEHP-induced toxicity to immature testes via the PGAM5–DRP1 interaction.
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Zhou, Xiazhu, Hong, Yifan, Chen, Jing, Yu, Yihang, Wang, Siyuan, Wei, Yuexin, Long, Chunlan, Shen, Lianju, Wu, Shengde, and Wei, Guanghui
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MITOCHONDRIA ,TESTIS ,REACTIVE oxygen species ,GONADS ,GERM cells - Abstract
Di-(2-ethylhexyl) phthalate (DEHP) is a widely used plasticizer that has been shown to impair male reproduction, but the potential mechanism underlying testicular injury caused by DEHP remains unclear. In vivo, rats were gavaged consecutively from postnatal day (PND) 21 to PND 31 with 0, 250, or 500 mg/kg DEHP for 10 days, and impaired mitochondria and increased necroptosis were observed in immature testes. In vitro, the GC-1 and GC-2 cell lines were exposed to monoethylhexyl phthalate (MEHP) at 100, 200 and 400 μM for 24 h, and this exposure induced oxidative stress damage, necroptosis and mitochondrial injury. Necroptosis and mitochondrial fission were inhibited by the reactive oxygen species (ROS) inhibitor acetylcysteine, and the imbalanced mitochondrial dynamics were rescued by the RIPK1 inhibitor necrostatin-1. Colocalization and co-IP analyses confirmed an interaction between dynamin-related protein 1 (DRP1) and phosphoglycerate mutase 5 (PGAM5), indicating that PGAM5 dephosphorylates DRP1 at serine 637 to induce mitochondrial fragmentation and thereby induces germ cell damage. Drug prediction with Connectivity Map (cMap) identified sulforaphane as a therapeutic drug. In summary, our findings indicate that DEHP triggers necroptosis and mitochondrial injury via a ROS storm in immature testes and that the PGAM5–DRP1 interaction is involved in this process. [Display omitted] • Necroptosis-induced mitochondrial damage caused DEHP-induced testicular injury. • ROS may be the driving forces for necroptosis and imbalanced mitochondrial dynamics. • PGAM5 promote mitochondrial fission via dephosphorylation of DRP1 S637. • CMap identified sulforaphane as a potential therapeutic drug. [ABSTRACT FROM AUTHOR]
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- 2024
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7. Endovascular thrombectomy versus standard bridging thrombolytic with endovascular thrombectomy within 4·5 h of stroke onset: an open-label, blinded-endpoint, randomised non-inferiority trial
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Mitchell, Peter J, Yan, Bernard, Churilov, Leonid, Dowling, Richard J, Bush, Steven J, Bivard, Andrew, Huo, Xiao Chuan, Wang, Guoqing, Zhang, Shi Yong, Ton, Mai Duy, Cordato, Dennis J, Kleinig, Timothy J, Ma, Henry, Chandra, Ronil V, Brown, Helen, Campbell, Bruce C V, Cheung, Andrew K, Steinfort, Brendan, Scroop, Rebecca, Redmond, Kendal, Miteff, Ferdinand, Liu, Yan, Duc, Dang Phuc, Rice, Hal, Parsons, Mark W, Wu, Teddy Y, Nguyen, Huy-Thang, Donnan, Geoffrey A, Miao, Zhong Rong, Davis, Stephen M, Desmond, Patricia, Yassi, Nawaf, Zhao, Henry, Williams, Cameron, Alemseged, Fana, Ng, Felix C, Yogendrakumar, Vignan, Bailey, Peter, De Villiers, Laetitia, Phan, Thanh, Thirugnanachandran, Tharani, Chong, Winston, Asadi, Hamed, Slater, Lee Anne, Manning, Nathan, Wenderoth, Jason, McDougall, Alan, Williams, Cameron, Cappelen-Smith, Cecilia, Whitley, Justin, Edwards, Leon, Esperon, Carlos Garcia, Spratt, Neil, Pepper, Elizabeth, Levi, Chris, Faulder, Ken, Harrington, Timothy, Krause, Martin, Waters, Michael, Fink, John, Ma, Gaoting, Shen, Xiangpeng, Song, Xiangkong, Gao, Yonglei, Guangxian, Nam, Guo, Zaiyu, Zhang, Heliang, Han, Hongxing, Wang, Hao, Liao, Geng, Zhang, Zhenyu, Li, Chaomao, Yang, Zhi, Cai, Chuwei, Huang, Chuming, and Hong, Yifan
- Abstract
The benefit of combined treatment with intravenous thrombolysis before endovascular thrombectomy in patients with acute ischaemic stroke caused by large vessel occlusion remains unclear. We hypothesised that the clinical outcomes of patients with stroke with large vessel occlusion treated with direct endovascular thrombectomy within 4·5 h would be non-inferior compared with the outcomes of those treated with standard bridging therapy (intravenous thrombolysis before endovascular thrombectomy).
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- 2022
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8. Wnt10a downregulation contributes to MEHP-induced disruption of self-renewal and differentiation balance and proliferation inhibition in GC-1 cells: Insights from multiple transcriptomic profiling.
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Hong, Yifan, Zhou, Xiazhu, Li, Qi, Chen, Jing, Wei, Yuexin, Wang, Siyuan, Zheng, Xiangqin, Zhao, Jie, Yu, Chengjun, Pei, Jun, Zhang, Jie, Long, Chunlan, Shen, Lianju, Wu, Shengde, and Wei, Guanghui
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PHTHALATE esters ,TRANSCRIPTOMES ,DOWNREGULATION ,GERM cells ,TESTIS injuries - Abstract
Di (2-ethylhexyl) phthalate (DEHP), one of phthalic acid esters, has been widely used in daily products. Its main metabolite, mono (2-ethylhexyl) phthalate (MEHP) was reported to possess higher testicular toxicity than DEHP. To explore the precise mechanism in MEHP-induced testis damage, multiple transcriptomic sequencing was employed in spermatogonia cell line GC-1 cells treated with MEHP (0, 100, and 200 μM) for 24 h. Integrative omics analysis and empirical validation revealed that Wnt signaling pathway was downregulated and wnt10a , one of hub genes, may be the key player in this process. Similar results were observed in DEHP-exposed rats. MEHP-induced disturbance of self-renewal and differentiation was dose-dependent. Moreover, self-renewal proteins were downregulated; the differentiation level was stimulated. Meanwhile, GC-1 proliferation was decreased. Stable transformation strain of wnt10a overexpression GC-1 cell line constructed from lentivirus was utilized in this study. The upregulation of Wnt10a significantly reversed the dysfunction of self-renewal and differentiation and promoted the cell proliferation. Finally, retinol, predicted to be useful in CONNECTIVITY MAP (cMAP), failed to rescue the damage caused by MEHP. Cumulatively, our findings revealed that the downregulation of Wnt10a induced the imbalance of self-renew and differentiation, and inhibition of cell proliferation in GC-1 cells after MEHP exposure. [Display omitted] • RNA-seqs showed Wnt pathway was crucial in MEHP-induced testis injury. • Wnt10a was the target of MEHP-induced germ cell damage. • Reduced Wnt10a disturbed self-renew, differentiation and proliferation in GC-1. • Retinol failed to rescue MEHP caused damage in vitro. [ABSTRACT FROM AUTHOR]
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- 2023
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9. A Mass-Ratiometry-Based CD45 Barcoding Method for Mass Cytometry Detection
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Meng, Hongu, Warden, Antony, Zhang, Lulu, Zhang, Ting, Li, Yiyang, Tan, Ziyang, Wang, Boqian, Li, Hongxia, Jiang, Hui, Shen, Guangxia, Hong, Yifan, and Ding, Xianting
- Abstract
Mass cytometry (CyTOF) is a critical cell profiling tool in acquiring multiparameter proteome data at the single-cell level. A major challenge in CyTOF analysis is sample-to-sample variance arising from the pipetting process, staining variation, and instrument sensitivity. To reduce such variations, cell barcoding strategies that enable the combination of individual samples prior to antibody staining and data acquisition on CyTOF are often utilized. The most prevalent barcoding strategy is based on a binary scheme that cross-examines the existence or nonexistence of certain mass signals; however, it is limited by low barcoding efficiency and high cost, especially for large sample size. Herein, we present a novel barcoding method for CyTOF application based on mass ratiometry. Different mass tags with specific fixed ratios are used to label CD45 antibody to achieve sample barcoding. The presented method exponentially increases the number of possible barcoded samples with the same amount of mass tags compared with conventional methods. It also reduces the overall time for the labeling process to 40 min and avoids the need for expensive commercial barcoding buffer reagents. Moreover, unlike the conventional barcoding process, this strategy does not pre-permeabilize cells before the barcoding procedure, which offers additional benefits in preserving surface biomarker signals.
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- 2019
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10. A Mass-Ratiometry-Based CD45 Barcoding Method for Mass Cytometry Detection
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Meng, Hongu, Warden, Antony, Zhang, Lulu, Zhang, Ting, Li, Yiyang, Tan, Ziyang, Wang, Boqian, Li, Hongxia, Jiang, Hui, Shen, Guangxia, Hong, Yifan, and Ding, Xianting
- Abstract
Mass cytometry (CyTOF) is a critical cell profiling tool in acquiring multiparameter proteome data at the single-cell level. A major challenge in CyTOF analysis is sample-to-sample variance arising from the pipetting process, staining variation, and instrument sensitivity. To reduce such variations, cell barcoding strategies that enable the combination of individual samples prior to antibody staining and data acquisition on CyTOF are often utilized. The most prevalent barcoding strategy is based on a binary scheme that cross-examines the existence or nonexistence of certain mass signals; however, it is limited by low barcoding efficiency and high cost, especially for large sample size. Herein, we present a novel barcoding method for CyTOF application based on mass ratiometry. Different mass tags with specific fixed ratios are used to label CD45 antibody to achieve sample barcoding. The presented method exponentially increases the number of possible barcoded samples with the same amount of mass tags compared with conventional methods. It also reduces the overall time for the labeling process to 40 min and avoids the need for expensive commercial barcoding buffer reagents. Moreover, unlike the conventional barcoding process, this strategy does not pre-permeabilize cells before the barcoding procedure, which offers additional benefits in preserving surface biomarker signals.
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- 2019
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11. Volume Polarization Holographic Recording in Phenanthrenequinone Doped Poly(MMA-co-BzMA) Photopolymer
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Fan, Fenglan, Liu, Ying, Hong, Yifan, Zang, Jinliang, Kang, Guoguo, Zhao, Tianbo, Tan, Xiaodi, and Shimura, Tsutomu
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Phenanthrenequinone (PQ) doped poly(methyl methacrylate) (PMMA) has been proven to be available material for volume polarization holographic recording. In our work, we introduced benzyl methacrylate (BzMA) into the PQ/PMMA to increase the concentration of PQ (up to 1.15 wt%) within the polymeric media. The enhancement of concentration of PQ brings a mean for improving performance of material. Compared with the pure PQ/PMMA, photoinduced birefringence and diffraction intensity were multiplied by introducing BzMA. This investigation can provide a significant foundation for improving polarization holographic characteristics of PQ/PMMA by modifying the chemical composition of the material.In our work, we introduced benzyl methacrylate (BzMA) into PQ/PMMA to increase the concentration of PQ (up to 1.15 wt%) within the polymeric media. The enhancement of concentration of PQ brings a means for improving performance of material. This is an efficient method to improve polarization holographic characteristics of PQ/PMMA.
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- 2018
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12. Investigation of faithful reconstruction in nonparaxial approximation polarization holography
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Hong, Yifan, Kang, Guoguo, Zang, Jinliang, Fan, Fenglan, Liu, Ying, Tan, Xiaodi, Shimura, Tsutomu, and Kuroda, Kazuo
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Faithful reconstruction, the polarization state of the diffraction wave being identical to that of the signal wave, is the primary target in polarization holography. With the large crossing angle between signal and reference wave, namely, nonparaxial approximation, attaining a balance between intensity and polarization grating is generally known as a prerequisite for faithful reconstruction. However, obtaining such a balance point within polarization-sensitive material has long been known as a challenging job, due to its extremely strict exposure condition. In this paper, we report on a smart method with the well-designed reference and reading wave to faithfully reconstruct the signal wave at an arbitrary exposure energy. Moreover, when the signal is circularly polarized, the diffraction efficiency can be precisely manipulated by changing the reading wave’s polarization state, which is favorable for optical storage and functional diffractive optical elements.
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- 2017
13. Dual-channel recording based on the null reconstruction effect of orthogonal linear polarization holography
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Zang, Jinliang, Kang, Guoguo, Li, Peng, Liu, Ying, Fan, Fenglan, Hong, Yifan, Huang, Yong, Tan, Xiaodi, Wu, An’an, Shimura, Tsutomu, and Kuroda, Kazuo
- Abstract
We report on dual-channel recording within polarization holography written by orthogonal linear polarization waves. The null reconstruction effect (NRE) of linear polarization holography was experimentally achieved at a large cross-angle of π/2 inside the polarization-sensitive media. Based on the NRE, two polarization encoded holograms were recorded in a dual-channel recording system with negligible inter-channel crosstalk. The two polarization multiplexed holograms could then be sequentially or simultaneously readout by shifting the polarization state of reference wave with the best signal-to-noise of 18:1 obtained within the experiment.
- Published
- 2017
14. Targeting leucine-rich repeat serine/threonine-protein kinase 2 sensitizes pancreatic ductal adenocarcinoma to anti-PD-L1 immunotherapy
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Sun, Kang, Zhang, Xiaozhen, Lao, Mengyi, He, Lihong, Wang, Sicheng, Yang, Hanshen, Xu, Jian, Tang, Jianghui, Hong, Zhengtao, Song, Jinyuan, Guo, Chengxiang, Li, Muchun, Liu, Xinyuan, Chen, Yan, Zhang, Hanjia, Zhou, Jingxing, Lin, Jieru, Zhang, Sirui, Hong, Yifan, Huang, Jinyan, Liang, Tingbo, and Bai, Xueli
- Abstract
Pancreatic ductal adenocarcinoma (PDAC) is not sensitive to immune checkpoint blockade therapy, and negative feedback of tumor immune evasion might be partly responsible. We isolated CD8+ T cells and cultured them in vitro. Proteomics analysis was performed to compare changes in Panc02 cell lines cultured with conditioned medium, and leucine-rich repeat kinase 2 (LRRK2) was identified as a differential gene. LRRK2 expression was related to CD8+ T cell spatial distribution in PDAC clinical samples and upregulated by CD8+ T cells via interferon gamma (IFN-γ) simulation in vitro.Knockdown or pharmacological inhibition of LRRK2 activated an anti-pancreatic cancer immune response in mice, which meant that LRRK2 acted as an immunosuppressive gene. Mechanistically, LRRK2 phosphorylated PD-L1 at T210 to inhibit its ubiquitination-mediated proteasomal degradation. LRRK2 inhibition attenuated PD-1/PD-L1 blockade-mediated, T cell-induced upregulation of LRRK2/PD-L1, thus sensitizing the mice to anti-PD therapy. In addition, adenosylcobalamin, the activated form of vitamin B12, which was found to be a broad-spectrum inhibitor of LRRK2, could inhibit LRRK2 in vivoand sensitize PDAC to immunotherapy as well, which potentially endows LRRK2 inhibition with clinical translational value. Therefore, PD-L1 blockade combined with LRRK2 inhibition could be a novel therapy strategy for PDAC.
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- 2023
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15. Exposure to DEHP induces testis toxicity and injury through the ROS/mTOR/NLRP3 signaling pathway in immature rats.
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Hong, Yifan, Zhou, Yu, Shen, Lianju, Wei, Yuexin, Long, Chunlan, Fu, Yan, Wu, Huan, Wang, Junke, Wu, Yuhao, Wu, Shengde, and Wei, Guanghui
- Subjects
CELLULAR signal transduction ,TESTIS injuries ,RATS ,SPRAGUE Dawley rats ,GERM cells ,SPERMATOGENESIS ,MALE reproductive organs - Abstract
As the most abundantly used phthalate derivative, di-(2-ethylhexyl) phthalate (DEHP) leads to reproductive disorders, especially in males. Testicular injury can be triggered when the testis is exposed to DEHP during the immature stage. However, the potential mechanism is largely unclear. In the present study, Sprague-Dawley rats were exposed to 0, 250 and 500 mg/kg/day DEHP from postnatal day (PND) 20 to PND 30. The spermatogonia cell line GC-1 and spermatocyte cell line GC-2 were exposed to different doses of monoethylhexyl phthalate (MEHP), a metabolite of DEHP. Testicular injury was observed. Oxidative stress was evaluated both in vivo and in vitro. Our results showed that after DEHP exposure, the testicular structure was damaged and spermatogenesis was disturbed. We also found that oxidative stress was increased, as indicated by the upregulation of the important factors in the antioxidant pathway. Furthermore, the expression of autophagy-related proteins was significantly downregulated. Autophagy inhibition led to activation of the pyroptosis pathway. Nucleotide-binding and oligomerisation (NOD) domain-like receptor (NLR) family pyrin domain (PYD)-containing 3 (NLRP3), Caspase-1 and cytokine interleukin-1β (IL-1β) were significantly upregulated. Additionally, an imbalance in self-renewal and differentiation was observed in germ cells after DEHP exposure, causing the cessation of germ cell development. In summary, these data suggest that DEHP exposure enhances oxidative stress, downregulates autophagy, induces NLRP3 inflammasome activation and subsequently triggers pyroptosis in vivo and in vitro, which provides novel insight into DEHP-related injury in immature testes in the context of pyroptosis. [Display omitted] • Exposure to DEHP could induce reproductive injury in immature testes. • The ROS/mTOR/NLRP3 signaling pathway participates in testicular injury induced by DEHP exposure. • DEHP exposure also increases the level of self-renewal and differentiation of GC-1 and GC-2 cells. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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16. Polystyrene microplastics disrupt the blood-testis barrier integrity through ROS-Mediated imbalance of mTORC1 and mTORC2.
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Wei, Yuexin, Zhou, Yu, Long, Chunlan, Wu, Huan, Hong, Yifan, Fu, Yan, Wang, Junke, Wu, Yuhao, Shen, Lianju, and Wei, Guanghui
- Subjects
MICROPLASTICS ,SERTOLI cells ,MICROFILAMENT proteins ,PROTEIN expression ,REACTIVE oxygen species - Abstract
It has been found that polystyrene microplastics (PS-MPs) exposure leads to decreased sperm quality and quantity, and we aim to explore the underlying mechanisms. Therefore, we gave 20 mg/kg body weight (bw) and 40 mg/kg bw 4 μm and 10 μm PS-MPs to male Balb/c mice by gavage. RNA sequencing of testes was performed. After PS-MPs exposure, blood-testis barrier (BTB) integrity was impaired. Since cytoskeleton was closely related to BTB integrity maintenance, and cytoskeleton disorganization could be induced by PS-MPs exposure in the testis, which resulted in the truncation of actin filaments and disruption of BTB integrity. Such processes were attributed to the differential expression of Arp3 and Eps8 (two of the most important actin-binding proteins). According to the transcriptome sequencing results, we examined the oxidative stress level in the testes and Sertoli cells. We found that PS-MPs exposure induced increased reactive oxygen species (ROS) level, which destroyed the balance between mTORC1 and mTORC2 (the mTORC1 activity was increased, while the mTORC2 activity was decreased). In conclusion, PS-MPs induced the imbalance of mTORC1 and mTORC2 via the ROS burst, and altered the expression profile of actin-binding proteins, resulting in F-actin disorganization and reduced expression of junctional proteins in the BTB. Eventually PS-MPs led to BTB integrity disruption and spermatogenesis dysfunction. [Display omitted] • PS-MPs cause blood-testis barrier disruption. • PS-MPs reduced junctional proteins expressions through F-actin disorganization. • PS-MPs -induce BTB disruption through ROS-mediated imbalance of mTORC1 and mTORC2. BTB impairment induced by MPs exposure was regulated by ROS-mediated imbalance of mTORC1 and mTORC2. [ABSTRACT FROM AUTHOR]
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- 2021
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17. Large etendue solid-state-lidar with MEMS-resonant mirror assisted diffractive beam steering by digital micro mirror device
- Author
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Ehmke, John, Lee, Benjamin L., Kang, Eunmo, Choi, Heejoo, Hellman, Brandon, Rodriguez, Joshua, Smith, Braden, Deng, Xianyue, Liu, Parker, Lee, Ted Lian-Tai, Evans, Erik, Hong, Yifan, Guan, Jiafan, Luo, Chuan, and Takashima, Yuzuru
- Published
- 2022
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18. Wnt5a Regulates Junctional Function of Sertoli cells Through PCP-mediated Effects on mTORC1 and mTORC2
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Fu, Yan, Wei, Yuexin, Zhou, Yu, Wu, Huan, Hong, Yifan, Long, Chunlan, Wang, Junke, Wu, Yuhao, Wu, Shengde, Shen, Lianju, and Wei, Guanghui
- Abstract
The blood–testis barrier (BTB) and apical ectoplasmic specialization (ES), which are synchronized through the crosstalk of Sertoli cells and Sertoli germ cells, are required for spermatogenesis and sperm release. Here, we show that Wnt5a, a noncanonical Wnt signaling pathway ligand, is predominately expressed in both the BTB and apical ES and has a specific expression pattern during the seminiferous epithelium cycle. We employed siRNA to knockdown Wnt5a expression in testis and Sertoli cells, and then identified elongated spermatids that lost their polarity and were embedded in the seminiferous epithelium. Moreover, phagosomes were found near the tubule lumen. These defects were due to BTB and apical ES disruption. We also verified that the expression level and/or location of BTB-associated proteins, actin binding proteins (ABPs), and F-actin was changed after Wnt5a knockdown in vivo and in vitro. Additionally, we demonstrated that Wnt5a regulated actin dynamics through Ror2-mediated mTORC1 and mTORC2. This study clarified the molecular mechanism of Wnt5a in Sertoli cell junctions through the planar cell polarity (PCP) signaling pathway. Our findings could provide an experimental basis for the clinical diagnosis and treatment of male infertility caused by Sertoli cell junction impairment.
- Published
- 2021
- Full Text
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19. Highly concentrated phenanthrenequinone-doped poly(MMA-co-ACMO) for volume holography
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Fan, Fenglan, Liu, Ying, Hong, Yifan, Zang, Jinliang, Kang, Guoguo, Zhao, Tianbo, and Tan, Xiaodi
- Abstract
Novel composite materials are synthesized by incorporating N-acryloylmorpholine (ACMO) in highly concentrated phenanthrenequinone (PQ) doped poly(methyl methacrylate) (PMMA). The photosensitizer concentration of PQ was increased from 0.7 wt. % to 1.8 wt. %. The doping of ACMO component results in a higher diffraction efficiency and photosensitivity than a typical PQ/PMMA system. The enhanced performance of the material may stem from the ACMO molecules, which might open a new route for improving the holographic performance of the PQ/PMMA photopolymer.
- Published
- 2018
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