Search

Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct biochemical processes into discrete subcellular locations. Microscopy1and biochemical fractionation coupled with mass spectrometry2–4have defined the proteomes of a variety of different organelles, but many intracellular compartments have remained refractory to such approaches. Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define the composition of cellular compartments in living cells5–7. Here we present a BioID-based map of a human cell on the basis of 192 subcellular markers, and define the intracellular locations of 4,145 unique proteins in HEK293 cells. Our localization predictions exceed the specificity of previous approaches, and enabled the discovery of proteins at the interface between the mitochondrial outer membrane and the endoplasmic reticulum that are crucial for mitochondrial homeostasis. On the basis of this dataset, we created humancellmap.org as a community resource that provides online tools for localization analysis of user BioID data, and demonstrate how this resource can be used to understand BioID results better.

The article discusses U.S. political poetry by poets Adrienne Rich and Audre Lorde including criticism, anger and hatred they received for their work, and contributions made by them in the American feminist movement.

This article investigates the role of solvation effects in the autocatalysis reaction of the epoxy–amine cure reaction. A single‐phase three component model was developed encompassing a two‐component reaction mix and a single polymeric product. The reaction was modelled as an SN2 reaction. Association of the nucleophile with each component in the reaction was defined via a binding constant. © 2004 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 42: 3579–3586, 2004

We describe evidence that serum samples from patients with chronic renal failure possess a constituent that interferes with the measurement of uric acid when using a method employing uricase with peroxidase-assisted detection of the hydrogen peroxide generated. The interference is also reflected in reduced recovery of both hydrogen and sarcosine when added to serum from patients with chronic renal failure. The interferent may be protein in nature or protein bound, as the poor recovery of hydrogen peroxide is seen in patients' serum before and after haemodialysis.

We describe the problems encountered with the adaptation of two enzymatic assays for uric acid to a centrifugal analyser. A method employing the uricase/catalase/aldehyde dehydrogenase enzyme system sometimes suffered from interference due to light-scattering species in samples and reagents. The second method employing the uricase/peroxidase system gave results that were lower than the comparison method.

The poem "On Purpose," by D. R. James is presented. First Line: What was I thinking; Last Line: of our rather common ancestors.

Presents the poem "On Not Being in Bangladesh," by D. R. James. First Line: I like writing about where I'm not, such as; Last Line: from all the places where I'm not.

Phosphoprotein recognition directs kinase-phosphatase interactions at multiple levels in the mammalian Hippo pathway.

The article presents the poem "Vast," by D.R. James. First Line: Just beyond Minneapolis-St. Paul, we've seemed; Last Line: from everything then reimaginable.

Presents the poem "I Don't Know the Biochemistry of a Hummingbird," by D. R. James. First Line: I can only wonder; Last Line: depletion?

Presents the poem "The Day I Got My Timing Down," by D. R. James. First Line: It was in that phase of pure; Last Line: gullible, stuttering face.

This is a multiauthor text from the craniofacial team at the Cleft Palate & Craniofacial Deformities Institute which is part of the Washington University Medical Center, St Louis, Missouri. It documents experience gained since 1978 in evaluating and providing care for 1013 patients with cleft lip and/or palate and other craniofacial deformities.