1. Piperine suppresses growth and migration of human breast cancer cells through attenuation of Rac1 expression.
- Author
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Buranrat, Benjaporn and Junking, Mutita
- Subjects
CANCER cells ,HUMAN migrations ,BREAST cancer ,CANCER cell migration ,HUMAN growth - Abstract
Objective: To investigate the effect of piperine on human breast cancer cells. Methods: The effect of piperine on proliferation and migration of human breast cancer cells, MCF-7 and MDA-MB-231, was investigated using colony formation assays, wound healing assays, Matrigel migration assays, flow cytometry, RT-qPCR, and Western blotting assays. Results: Piperine inhibited the growth of MCF-7 and MDA-MB-231 cells and suppressed colony formation. Cell reduction at the G
0 / G1 phase and cell arrest at the G2 /M phase were observed in breast cancer cells. However, the significant effect was only demonstrated in MDA-MB-231 cells. Moreover, cancer cell migration was suppressed by piperine at low concentration. RT-qPCR and Western blotting assays showed that piperine downregulated Rac1 gene and protein expression. Conclusions: Piperine could inhibit growth and migration of breast cancer cells by reducing Rac1 gene and protein expression. [ABSTRACT FROM AUTHOR]- Published
- 2022
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