A sensitive hydrazine electrochemical sensor based on Zinc oxide nano-wires (ZnO NWs) synthesized from low temperature hydrothermal method has been fabricated using an add-on technique. The morphology, crystalline structures, and composition of synthesized ZnONWswere characterized by scanning electron microscopy,X-ray powder diffraction, energy dispersive spectroscopy, and high-resolution transmission electron microscopy. The batch-made ZnO NWs were used as electrocatalytic material andmodified on the gold electrode surface through a printing-like method via Nafion to obtain Nafion/ZnO NWs/Au hydrazine electrochemical sensor. To optimize performance, the effect of the ZnO modified quantity on sensitivity was evaluated by cyclic voltammetry, electrochemical impedance spectroscopic, and single-potential amperometry techniques. The results show that the optimum ZnO NWs modified quantity is about 170 µg ⋅ cm-2. The optimized Nafion/ZnO NWs/Au hydrazine electrochemical sensor has a high sensitivity of 3.8 µA⋅µM-1 ⋅ cm-2, a short response time of 3 s, and a very low detection limit of 0.0144 µMwith a linear hydrazine concentration response range from 3.0 to 562 µM. [ABSTRACT FROM AUTHOR]