63 results on '"Lee, Jae Kwan"'
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2. Sequential‐dip‐coating processed mixed organic and inorganic perovskite film from halide‐free lead precursor for efficient perovskite solar cells
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Irshad, Zobia, Adnan, Muhammad, Kim, Ho‐Joong, and Lee, Jae Kwan
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A novel all‐sequential‐dip‐coated (SDC) deposited FA1MA1−yPbI3−xBrxperovskite films have been synthesized from an aqueous non‐halide lead precursor towards an environmentally benign, cost‐effective, and efficient approach for high‐performance perovskite solar cells (PrSCs). The mixed‐cationic FA1MA1−yPbI3−xBrxperovskite layers ensure the fractional incorporation of Br into the perovskite crystal lattice. The insertion of Br contents was regulated by modulating the FABr concentration into the FABr/MAI mixed‐cationic precursor solution. The incorporation of minor FABr into MAI helps to improve the surface coverage and crystallinity of the synthesized per‐ovskite layer in contrast to the other perovskite films prepared with higher FABr content under the same environment. The PrSCs with these FA1MA1−yPbI3−xBrxperovskite layers displayed good de‐vice performances and stability with a PCE of 11.1%. These outcomes reveal that the synthesis of FA1MA1−yPbI3−xBrxperovskite films with the SDC approach is more efficient because of the use of environmentally benign solvents for synthesizing lead and perovskite layers. The development of FA1MA1−yPbI3−xBrxperovskite films through an all‐sequential‐dip‐coated (SDC) deposition method marks a significant advancement in the field of perovskite solar cells (PrSCs). This novel approach utilizes an aqueous non‐halide lead precursor, promoting an environmentally friendly and cost‐effective alternative to traditional methods. The strategic modulation of FABr concentration in the precursor solution enables precise control over the bromine content within the perovskite crystal lattice, enhancing surface coverage and crystallinity. Such improvements are crucial for achieving high device performance and stability. The SDC method not only demonstrates increased efficiency due to the use of benign solvents but also shows promise for scaling up to large‐area PrSC devices, overcoming limitations associated with conventional spin‐casting techniques. This innovative synthesis strategy could potentially lead to more sustainable and scalable production of high‐performance PrSCs, contributing to the advancement of solar energy technology.
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- 2024
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3. Effect of Weissella cibaria on the reduction of periodontal tissue destruction in mice.
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Kim, Jae‐Won, Jung, Bo Hyun, Lee, Jong Hun, Yoo, Ki‐Yeon, Lee, Heesu, Kang, Mi‐Sun, Lee, Jae‐Kwan, Kim, Jae-Won, Yoo, Ki-Yeon, Kang, Mi-Sun, and Lee, Jae-Kwan
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Background: Periodontitis is associated with dysbiosis of microbial flora in the oral cavity. We evaluated the effects of an oral care probiotic, Weissella cibaria CMU, on periodontal tissue destruction and regulation of inflammatory cytokines in mice with ligature-induced periodontitis (LIP).Methods: Fourteen-day LIP model was used. Ninety animals were randomly divided into six groups: negative control (Ctrl), positive control (LIP/Ctrl), PBS-treated (LIP/PBS), W. cibaria-low (1 × 107 colony forming unit (CFU)/d; LIP/WC-L), W. cibaria-medium (1 × 108 CFU/d; LIP/WC-M), and W. cibaria-high (1 × 109 CFU/d; LIP/WC-H). After the 14-day treatment, alveolar bone loss was determined using micro-computed tomography. The gingival tissue and serum samples from Ctrl, LIP/Ctrl, and LIP/WC-H groups were immunoassayed for cytokines. Measurements of Porphyromonas gingivalis, total bacteria, and W. cibaria in the gingiva were performed using real-time polymerase chain reaction.Results: Mice in the LIP/WC-H group showed significant reduction in alveolar bone loss at the distal aspect of the ligatured teeth compared to those in the LIP/Ctrl group. There was a dose-dependent reduction (non-significant) in periodontal tissue destruction with increased W. cibaria concentration. Pro- and anti-inflammatory cytokines were significantly lower in LIP/WC-H than in LIP/Ctrl. The LIP/WC-H group showed significantly fewer total bacteria compared to the LIP/Ctrl group but it was similar to that in Ctrl groups, and P. gingivalis was not detected in the gingival tissue.Conclusions: W. cibaria CMU reduces periodontal tissue destruction apparently by regulating the production of inflammatory cytokines and by reducing oral bacteria in a model for periodontal disease. [ABSTRACT FROM AUTHOR]- Published
- 2020
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4. Learning curve could affect oncologic outcome of minimally invasive radical hysterectomy for cervical cancer.
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Kim, Seongmin, Min, Kyung Jin, Lee, Sanghoon, Hong, Jin Hwa, Song, Jae Yun, Lee, Jae Kwan, and Lee, Nak Woo
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Recent prospective studies have shown poorer oncologic outcomes following minimally invasive surgery, which has led many surgeons to deeply inspect their practices. We reviewed our experience and evaluated the results of radical hysterectomy in patients with early stage cervical cancer. This retrospective study included patients with early stage cervical cancer (Ia1 - IIa1) who were treated with radical hysterectomy from May 2006 to Dec 2016. Patients were divided into three groups according to the surgical approach: radical abdominal hysterectomy (RAH), laparoscopic radical hysterectomy (LRH), and robot-assisted radical hysterectomy (RRH). Learning curves of each type of surgery were obtained using the cumulative sum method. Survival rates were compared using Kaplan–Meier curves. To analyze the learning curve of a single surgeon, 89 patients were selected from the whole population. Learning curves of each group showed two distinct phases. The minimum number of cases required to achieve surgical improvement were 16 in RAH, 13 in LRH, and 21 in RRH. Progression-free survival (PFS) and overall survival did not vary between RAH and minimally invasive surgery (MIS) (p =.828 and p =.757, respectively). However, when stratified by the phases of the learning curves, patients included in the early phase of MIS showed a poorer PFS (p =.014). Surgical proficiency could significantly affect the oncologic outcome in MIS. A prospective study regarding sufficient surgical competence is necessary for elaborate analysis of the feasibility of minimally invasive radical hysterectomy. [ABSTRACT FROM AUTHOR]
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- 2021
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5. Methods on load estimation for the implementation assessment in the management of total maximum daily loads
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Park, Jae Hong, Oh, Seung Young, Ryu, Jichul, Lee, Jae Kwan, and Shin, Dong Seok
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The annual amount of water pollution discharge load is utilized to assess its implementation of the load allotment on each unit watershed in the management of total maximum daily loads (TMDLs). The amount of the discharge load might be changed as the rainfall changes in the area with the combined sewer system. In order to evaluate the implementation properly, the effects of rainfall changes should be considered in the estimation of the discharge load. It is necessary to standardize the annual discharge load to be calculated at the same rainfall condition as that of the reference year. But the calculation process is very difficult and might have some limitations. This study investigated and developed two methods in order to estimate the discharge load in a relatively simple way. The load conversion method (LCM) is designed to calculate the differences in discharge due to rainfall changes and to convert the discharge load of the current rainfall condition into that of the reference rainfall condition. The multi-regression equation method (MREM) is to predict the discharge load directly on reference rainfall conditions using a multi-regression equation. These methods were applied to examine the calculation results. LCM showed a more precise result with an error of –0.06%, while MREM –0.18%. Judging from the application results of this study LCM may be useful as a tool for estimating the discharge load for evaluation preferably in the TMDL process.
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- 2021
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6. Methodology for determining total phosphorus target water quality in total maximum daily loads
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Park, Jae Hong, Shin, Dong Seok, and Lee, Jae Kwan
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This study presents the principles and methods used in set target water quality values of total phosphorus (TP) in three major rivers of South Korea, such as Nakdong, Geum, and Yeongsan/Seomjin River. The QUALKO model (modified QUAL2E) was applied to establish the target water quality. According to the findings, while determining total maximum daily load (TMDL) targets, the intended use of water, current water quality, load density, and future pollutant elimination plans should be considered. If a watershed fails to meet the target quality standard, then the appropriate plan for the area should be established. In areas where small amounts of pollutants are released, development should be allowed within the limits of compliance with the water quality standards, targeting both development and water quality protection, which is the basic concept of TMDLs. Heavily polluted areas tend to continue releasing large amounts of pollutants, whereas others release only small amounts. The TMDL system is designed to address the problem by encouraging heavily polluted areas to reduce pollutant emission while allowing others to release some for development. The recommendations of this paper provide a reference to the development of a methodology for determining TP water quality considering TMDL.
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- 2020
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7. Effect of Weissella cibariaon the reduction of periodontal tissue destruction in mice
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Kim, Jae‐Won, Jung, Bo Hyun, Lee, Jong Hun, Yoo, Ki‐Yeon, Lee, Heesu, Kang, Mi‐Sun, and Lee, Jae‐Kwan
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Periodontitis is associated with dysbiosis of microbial flora in the oral cavity. We evaluated the effects of an oral care probiotic, Weissella cibariaCMU, on periodontal tissue destruction and regulation of inflammatory cytokines in mice with ligature‐induced periodontitis (LIP). Fourteen‐day LIP model was used. Ninety animals were randomly divided into six groups: negative control (Ctrl), positive control (LIP/Ctrl), PBS‐treated (LIP/PBS), W. cibaria‐low (1 × 107colony forming unit (CFU)/d; LIP/WC‐L), W. cibaria‐medium (1 × 108CFU/d; LIP/WC‐M), and W. cibaria‐high (1 × 109CFU/d; LIP/WC‐H). After the 14‐day treatment, alveolar bone loss was determined using micro‐computed tomography. The gingival tissue and serum samples from Ctrl, LIP/Ctrl, and LIP/WC‐H groups were immunoassayed for cytokines. Measurements of Porphyromonas gingivalis, total bacteria, and W. cibariain the gingiva were performed using real‐time polymerase chain reaction. Mice in the LIP/WC‐H group showed significant reduction in alveolar bone loss at the distal aspect of the ligatured teeth compared to those in the LIP/Ctrl group. There was a dose‐dependent reduction (non‐significant) in periodontal tissue destruction with increased W. cibariaconcentration. Pro‐ and anti‐inflammatory cytokines were significantly lower in LIP/WC‐H than in LIP/Ctrl. The LIP/WC‐H group showed significantly fewer total bacteria compared to the LIP/Ctrl group but it was similar to that in Ctrl groups, and P. gingivaliswas not detected in the gingival tissue. W. cibariaCMU reduces periodontal tissue destruction apparently by regulating the production of inflammatory cytokines and by reducing oral bacteria in a model for periodontal disease.
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- 2020
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8. Efficient Planar Heterojunction Inverted Perovskite Solar Cells with Perovskite Materials Deposited Using an Aqueous Non‐HalideLead Precursor
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Irshad, Zobia, Adnan, Muhammad, and Lee, Jae Kwan
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Efficient planar heterojunction inverted perovskite solar cells (PrSCs) were fabricated with MAPbI3perovskite layers prepared by using an aqueous Pb(NO3)2as a facile, cost‐effective, and an environmentally benign approach. Especially, the MAPbI3perovskite film fabricated from aqueous Pb(NO3)2on the MoO3/PEDOT:PSS hole transporting material bilayer exhibited better crystallinity, morphology, and performance. Efficient planar heterojunction inverted PrSCs with MAPbI3perovskite layers prepared by using aqueous Pb(NO3)2as a facile, cost‐effective, and environmentally benign approach were demonstrated. Especially, the MAPbI3perovskite film fabricated from aqueous Pb(NO3)2on the MoO3/PEDOT:PSS HTM bilayer exhibited better crystallinity, morphology, and performance.
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- 2020
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9. Burden of Human papillomavirus (HPV)-related disease and potential impact of HPV vaccines in the Republic of Korea
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Kim, Young-Tak, Serrano, Beatriz, Lee, Jae-Kwan, Lee, Hyunju, Lee, Shin-Wha, Freeman, Crystal, Oh, Jin-Kyoung, Alemany, Laia, Bosch, Francesc-Xavier, and Bruni, Laia
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We aimed to review the burden and the potential impact of human papillomavirus (HPV) vaccines on HPV-related diseases in the Republic of Korea and to discuss cervical cancer prevention practices in this country.
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- 2019
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10. Prevalence of human papillomavirus-related diseases in the Republic of Korea: a cross-sectional study
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Oh, Jin-Kyoung, Choi, Hwa Young, Han, Minji, Lee, Jae-Kwan, Min, Kyung-Jin, and Ki, Moran
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ObjectiveWe aimed to evaluate trends in the prevalence of human papillomavirus (HPV)-related diseases in the era before the introduction of organised HPV vaccination programmes in the Republic of Korea.MethodsThis cross-sectional study used National Health Insurance Service data from 2002 to 2015 and included participants who were diagnosed with the following HPV-related diseases (codes from the International Classification of Diseases, 10th Revision): genital warts (A63.0); cancer in the head and neck (C00–C10), anus (C21), vulva (C51), vagina (C52), cervix uteri (C53) and penis (C60); carcinoma in situ (CIS) of the lip/oral cavity/pharynx (D00.0), anus (D01.3), cervix (D06), vulva (D07.1), vagina (D07.2) and penis (D07.4); benign neoplasms of the larynx (D14.1); and dysplasia of the cervix (N87), vagina (N89) and vulva (N90). For each diagnosis, the fraction of cases attributable to HPV in Korea was assessed based on the percentages of diseases attributable to HPV reported in some international studies. The age-standardised prevalence was estimated using the direct population-based method.ResultsThe overall age-standardised prevalence of HPV-related diseases increased from 2002 to 2015, mainly due to increased prevalence of genital warts in men and cervical dysplasia and CIS in women. In women, genital wart prevalence increased from 2002 (24.4 per 100 000) to 2011 (57.1) and then decreased until 2015 (53.5); in men, the prevalence increased steadily from 2002 (22.9) to 2015 (109.4). The prevalence of cervical dysplasia and CIS increased (from 86.5 in 2002 to 484.5 in 2015, and from 60.3 in 2002 to 114.9 in 2015, respectively), but that of cervical cancer decreased (from 120.0 in 2002 to 106.9 in 2015).ConclusionsNon-organised HPV vaccination and organised cervical cancer screening may have contributed to the downward trend in genital wart prevalence and the upward trend in cervical abnormalities among women.
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- 2019
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11. Effect of photodynamic therapy according to differences in photosensitizers on Staphylococcus aureus biofilm on titanium.
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Park, Geun Hee, Lee, Si Young, Lee, Jong-Bin, Chang, Beom-Seok, Lee, Jae-Kwan, and Um, Heung-Sik
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• The ability of photodynamic therapy to control oral biofilms was investigated using titanium surfaces. • Use of toluidine blue O (TBO) was compared with methylene blue using 670 nm irradiation. • Data suggest TBO to be the more effective agent. This study aimed to evaluate the antimicrobial effect of photodynamic therapy (PDT) against Staphylococcus aureus biofilm on a titanium surface and to compare the differences in the effect of PDT using toluidine blue O (TBO) and methylene blue (MB) as a photosensitizer. The bacterial strain S. aureus ATCC 25,923 was used. Sandblasted and acid-etched (SLA) disks were divided into the following six groups: phosphate buffer saline (PBS), TBO, MB, PBS with laser (PBS + L) , TBO with laser (TBO + L) , and MB with laser (MB + L). The laser group samples were irradiated by a cold diode laser for 60 s. After treatment, the number of surviving bacteria was calculated by counting the colony-forming units (CFUs) and confocal laser scanning microscopy (CLSM) was applied to observe the bacteria on the disk surface. The TBO + L and MB + L groups showed significantly lower CFU/ml than the other groups (p < 0.01). The TBO + L group showed significantly lower CFU/ml than the MB + L group (p = 0.032). There was no significant difference between the PBS, TBO, MB, and PBS + L groups. Within the limitations of this in vitro study, PDT with TBO and MB can effectively reduce S. aureus biofilm on SLA titanium surfaces. TBO is more effective than MB as a photosensitizer. PDT with TBO may be applied to the treatment of peri‑implant disease in the future. [ABSTRACT FROM AUTHOR]
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- 2023
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12. Combined effect of diet and cervical microbiome on the risk of cervical intraepithelial neoplasia.
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Seo, Sang-Soo, Oh, Hea Young, Lee, Jae-Kwan, Kong, Ji-Sook, Lee, Dong Ock, and Kim, Mi Kyung
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Summary Background & aims Several food groups or dietary factors and the cervical microbiota may be involved in cervical carcinogenesis, but the evidence is not clear yet. We aimed to assess the association between dietary pattern and cervical intraepithelial neoplasia (CIN) and the combined effect of dietary pattern and cervical microbiome on the risk of CIN. Methods The cervical microbiota and diet assessed by pyrosequencing and a food-frequency questionnaire, respectively, of 65 women with CIN and 72 control women were used in this study. Principal component analysis and cluster analysis were used to identify dietary patterns and microbiome community types, respectively. The association between dietary pattern and CIN risk was assessed using multivariable logistic regression analysis. The combined effect of dietary pattern and microbiome on CIN risk was determined using relative excess risk due to interaction (RERI) and synergy index (S). Results Two dietary patterns and four community types were identified: prudent diet characterized by higher intake of vegetables and fishes; semi-Western diet characterized by higher intake of bread, dairy products, eggs, and soft drinks and relatively higher fat intake ratio; and Lactobacillus crispatus– , L . iners– , Atopobium vaginae– , and Prevotella bivia– dominant types. The high-scoring group of participants with a semi-Western diet had a higher risk of CIN (odds ratio [OR] 3.44, 95% confidence interval [CI] 1.11–10.7, p = 0.03), compared with the low or medium-scoring group of those with a semi-Western diet. L . iners– dominant (OR 6.39, 95% CI 1.52–26.7, p = 0.01) and A . vaginae– dominant (OR 4.99, 95% CI 1.17–21.3, p = 0.03) dominant types had a higher risk of CIN, compared with the L . crispatus– dominant type. The synergistic effect of semi-Western diet and A . vaginae– dominant type on CIN risk was observed (OR 20.8, 95% CI 2.21–195.6, p = 0.01, RERI/S 9.64/1.96). Conclusions Our findings suggest that semi-Western diet and its combination with A . vaginae– dominant microflora may represent an important risk factor for cervical neoplasia. [ABSTRACT FROM AUTHOR]
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- 2016
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13. Efficient Synthesis and Characterization of Solvatochromic Fluorophore
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Adnan, Muhammad, Kim, Hyeong Seok, Jeong, Hanbin, Ko, Haye Min, Woo, Sang Kook, and Lee, Jae Kwan
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A highly efficient designing and synthesis of an aryl‐substituted carbazole‐based fluorescent material (MeOCbzBP) has been carried out viatandem carbon–carbon (C–C)/carbon–nitrogen (C–N) bond formation by multiple Suzuki couplings and Cadogan cyclization followed by C–N cross‐couplings. Various organic solvents have been used to investigate photophysical, solvatofluorochromism, and photoluminescence characteristics of MeOCbzBP. The MeOCbzBPshowed a reasonable redshifted absorption in UVregion and photoluminescence enhancements in tetrahydrofuran/water mixtures. Furthermore, we also employed various theoretical functions to optimize the molecule both in the ground and excited states. A well‐known density functional theory was employed in a solvent phase with B3LYP/6‐31G(d) level of theories. We believe that the MeOCbzBPwill provide a better platform to investigate the novel applications in the field of medicinal chemistry and chemical biology. A highly efficient designing and synthesis of an aryl‐substituted carbazole‐based material (MeOCbzBP) viatandem carbon–carbon (C–C)/carbon–nitrogen (C–N) bond formation by multiple Suzuki couplings and Cadogan cyclization followed by C–N cross‐coupling was carried out and investigated for solvatofluorochromism with its photophysical and photoluminescence characteristics in various organic solvents.
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- 2017
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14. Long-term clinical outcomes of the single-incision technique for implantation of implantable venous access ports via the axillary vein
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Seo, Tae-Seok, Song, Myung Gyu, Kim, Jun Suk, Choi, Chul Won, Seo, Jae Hong, Oh, Sang Cheul, Kang, Eun Joo, Lee, Jae-Kwan, and Lee, Sung-yong
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Purpose To evaluate long-term clinical outcomes and complications of the single-incision technique for implantation of totally implantable venous access ports (TIVAPs) via the axillary vein.Materials and Methods A total of 932 TIVAPs were placed in 927 patients between May 2012 and October 2014 using a single-incision technique. Patients included 620 men and 307 women with a mean age of 60.0 years. TIVAPs were placed via the left (n = 475) and right (n = 457) axillary veins after making a single oblique vertical incision and medial side pocket without subcutaneous tunneling. We retrospectively reviewed medical records to evaluate status of the patients and TIVAPs, complications, and reasons for explantation. In patients who still had a TIVAP in place, we calculated the duration of TIVAP use from the cut-off day of November 1, 2015.Results Clinical follow-up was obtained for a total device service period of 311,069 days with a median indwelling time of 467 days (range: 3-1097 days). A total of 37 (4.0%) complications developed. Early complications (n = 4) were one case each of stenosis of the brachiocephalic vein by tumor growth, thrombosis of axillary vein, intravascular migration, and malfunction depending on patient's position. Late complications (n = 33) were suspected catheter-related blood stream infection (n = 23), local infection of the pocket (n = 4), symptomatic stenosis and thrombosis of central vein (n = 4), malfunction by fibrin sleeve (n = 1), and intravascular migration (n = 1).Conclusions A single-incision technique for TIVAP implantation via the axillary vein seems to be safe with a low risk of complication.
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- 2017
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15. Surface Improvement of TiO2 Nanotube Arrays for Dental Implant
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Kim, Wan Tae, Lee, Jae Kwan, Jang, In San, Choi, Dong Soon, and Choi, Won Youl
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Highly ordered TiO
2 nanotube arrays are very attractive to the dental implant due to microstructural advantage for drug loading. We have fabricated the highly ordered TiO2 nanotube arrays on the surface of the dental implant. The surface of TiO2 nanotube arrays grown by normal anodic oxidation was not clean and the window of TiO2 nanotube was closed. These closed nanotubes decrease the surface area to load the drug and also decrease the osseointegration performances. To obtain the clean surface of TiO2 nanotube arrays, two-step anodic oxidation was used. The microstructures of TiO2 nanotube arrays from two-step anodic oxidation were compared with those from normal anodic oxidation. The length and diameter of TiO2 nanotube arrays with anodizing time were measured. TiO2 nanotube arrays grown by two-step anodic oxidation had the clean surface and the diameter of TiO2 nanotubes was ~100 nm at anodizing conditions of 60V and 20 min. It was applied to the surface of dental implant to improve the osseointegration. The improved osseointegration was observed by micro CT analysis. TiO2 nanotube arrays had a promising microstructure to load some drugs such as BMP-2 and anti-inflammatory.- Published
- 2017
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16. Pregnancy Outcomes and Risk Factors for Uterine Rupture After Laparoscopic Myomectomy: A Single-Center Experience and Literature Review.
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Koo, Yu-Jin, Lee, Jae-Kwan, Lee, Yoo-Kyung, Kwak, Dong-Wook, Lee, In-Ho, Lim, Kyung-Taek, Lee, Ki-Heon, and Kim, Tae-Jin
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Study Objective To evaluate pregnancy outcomes after laparoscopic myomectomy (LSM), focusing on the risk of uterine rupture. Design Retrospective cohort study (Canadian Task Force classification III). Setting University hospital. Patients Of 676 women who visited the obstetrics department for a pregnancy after undergoing LSM performed at the same center between 1994 and 2012, we included the 523 women who had follow-up through the end of pregnancy. Interventions All patients underwent LSM, and their medical charts were retrospectively reviewed. Measurements and Main Results Multiple myomas were removed in 35.2% of cases, intramural-type lesions occurred in 46.5% of cases, and the mean myoma diameter was 4.9 cm. Pregnancy outcomes after LSM included 400 (76.5%) full-term deliveries and 100 (19.1%) vaginal deliveries, with other adverse outcomes being no different than the general population. The mean interval between LSM and pregnancy was 14 months, and only 3 (0.6%) cases of uterine rupture occurred during pregnancy. In analysis, by reviewing the published cases of uterine rupture, we found that the mean diameter, myoma number and type, and the rate of uterine suture were similar between the ruptured cases and all of our cases of LSM. Conclusion LSM can be safely used in women of reproductive age who want to become pregnant. Uterine rupture occurs in rare cases, regardless of myoma features, but further large-scale studies are required to ascertain the detailed effects of various surgical techniques. [ABSTRACT FROM AUTHOR]
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- 2015
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17. Antimicrobial photothermal therapy using diode laser with indocyanine green on Streptococcus gordonii biofilm attached to zirconia surface.
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Shim, Sang Ho, Lee, Si Young, Lee, Jong-Bin, Chang, Beom-Seok, Lee, Jae-Kwan, and Um, Heung-Sik
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• S. gordonii was significantly reduced in the PDT, PTT, and CHX groups. • L and ICG groups showed no significant reduction in S. gordonii. • CHX, PDT, and PTT groups showed the highest number of dead bacteria. The purpose of this study was to evaluate the antimicrobial effects of photothermal therapy using indocyanine green (ICG) and an 810-nm infrared diode laser on Streptococcus gordonii biofilm attached to zirconia surfaces in vitro. A biofilm was formed using the static method on zirconia disks placed in a 24-well plate. The biofilms were subdivided into the following six treatment groups: control, commercial photodynamic therapy (PDT), chlorhexidine gluconate (CHX), laser only (L, 810-nm infrared diode), ICG, and laser with ICG (PTT). After treatment, each disk was agitated and the solution with detached bacteria was spread directly on a blood agar plate. Cells were cultured under anaerobic conditions and colony-forming units were counted. Confocal laser-scanning microscopy was used to assess the survival according to the height of the biofilm. The PTT, PDT, and CHX groups showed a significant reduction in S. gordonii viability (p <0.05), while the L and ICG groups showed no significant difference compared to the control group (p = 0.32, p = 0.97; respectively). In confocal laser-scanning microscopy images, the PTT, PDT, and CHX groups presented most of the dead bacteria in both the upper and lower levels of biofilm. Within the limitations of this in vitro study, PTT with ICG was effective in significantly reducing the viability of S. gordonii bacteria on zirconia. Further studies are needed to establish a standardized PTT protocol to treat peri‑implant diseases. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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18. Prognostic value of total lesion glycolysis measured by 18F-FDG PET/CT in patients with locally advanced cervical cancer
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Hong, Jin Hwa, Jung, Un Suk, Min, Kyung Jin, Lee, Jae Kwan, Kim, Sungeun, and Eo, Jae Seon
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- 2016
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19. Characteristics of actinomycetes producing geosmin in Paldang Lake, Korea
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Park, Tae-Jin, Yu, Mi-Na, Kim, Hye-Sung, Cho, Hang-Soo, Hwang, Moon-Young, Yang, Hyung-Jae, Lee, Jae-Chan, Lee, Jae-Kwan, and Kim, Shin-Jo
- Abstract
AbstractGeosmin and 2-methylisoborneol (2-MIB) are organic compounds known to cause earthy and musty odors in drinking water. In Korea, during the summer of 2012, a geosmin outbreak in the drinking water supply prompted further research to identify the source of the odorous materials, since these occurrences were not well understood or monitored. The purpose of this study was to investigate how important a role actinomycetes play in the production of geosmin and 2-MIB in Paldang Lake by measurement of microbial density (the number of colony-forming units), pyrosequencing of 16S rRNA genes, and determination of the prevalence of the geosmin synthase gene. Three sampling sites (P1, P2, and P3) were selected. The increase in geosmin concentration was paralleled by an increase in actinomycetes (Streptomycesspp.) and cyanobacteria (Anabaenaspp.) populations. The bacterial communities in Paldang Lake were characterized by 454 pyrosequencing based on 16S rRNA gene sequences. P1 (North Han River) showed the highest density of actinomycetes, followed by P2 (Paldang Dam), and P3 (South Han River). The density of Streptomycesspp. increased at P1, while that of cyanobacteria increased at P3. No significant density change was noted in those two species at P2, a confluence of two rivers. The genes producing geosmin or 2-MIB were detected in 60 single strains that were isolated from water samples in Paldang Lake. This study confirmed that Streptomycesspp. significantly affect the production of geosmin that causes odors in Paldang Lake.
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- 2016
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20. Characteristics of actinomycetes producing geosmin in Paldang Lake, Korea
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Park, Tae-Jin, Yu, Mi-Na, Kim, Hye-Sung, Cho, Hang-Soo, Hwang, Moon-Young, Yang, Hyung-Jae, Lee, Jae-Chan, Lee, Jae-Kwan, and Kim, Shin-Jo
- Abstract
Geosmin and 2-methylisoborneol (2-MIB) are organic compounds known to cause earthy and musty odors in drinking water. In Korea, during the summer of 2012, a geosmin outbreak in the drinking water supply prompted further research to identify the source of the odorous materials, since these occurrences were not well understood or monitored. The purpose of this study was to investigate how important a role actinomycetes play in the production of geosmin and 2-MIB in Paldang Lake by measurement of microbial density (the number of colony-forming units), pyrosequencing of 16S rRNA genes, and determination of the prevalence of the geosmin synthase gene. Three sampling sites (P1, P2, and P3) were selected. The increase in geosmin concentration was paralleled by an increase in actinomycetes (Streptomycesspp.) and cyanobacteria (Anabaenaspp.) populations. The bacterial communities in Paldang Lake were characterized by 454 pyrosequencing based on 16S rRNA gene sequences. P1 (North Han River) showed the highest density of actinomycetes, followed by P2 (Paldang Dam), and P3 (South Han River). The density of Streptomycesspp. increased at P1, while that of cyanobacteria increased at P3. No significant density change was noted in those two species at P2, a confluence of two rivers. The genes producing geosmin or 2-MIB were detected in 60 single strains that were isolated from water samples in Paldang Lake. This study confirmed that Streptomycesspp. significantly affect the production of geosmin that causes odors in Paldang Lake.
- Published
- 2016
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21. Organic–Inorganic Hybrid Ternary Bulk Heterojunction of Nanostructured Perovskite–Low Bandgap Polymer–PCBM for Improved Efficiency of Organic Solar Cells
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Jeong, Hanbin and Lee, Jae Kwan
- Abstract
A new organic–inorganic ternary bulk heterojunction (TBHJ) hybrid configuration comprised of nanostructured (CH3)3NHPbI3(MAPbI3) perovskite–low bandgap PCPDTBT–PCBM was investigated. Well-organized TBHJ films were readily prepared by sequential spin-casting of sparsely covered MAPbI3nano dots and PCPDTBT–PCBM bulk heterojunction (BHJ) composites on ITO/PEDOT:PSS substrates. The TBHJ hybrid device configuration comprising diiooctane (DIO) treated MAPbI3perovskite nano dots and a PCPDTBT–PCBM BHJ composite processed with DIO additive exhibited excellent performances. The DIO additive played a key role in developing perovskite structures of MAPbI3nano dots and induced the (110) directional crystallinity growth of longitudinal constructive morphologies such as nano rods. The improved photocurrent and fill factor compared to those of conventional BHJ devices led to an increase in efficiency of ∼28%. This improved photovoltaic performance originated from the higher quantum efficiencies contributed by the charge transfer from nanostructured MAPbI3perovskite to PCBM. These TBHJs composed of nanostructured MAPbI3perovskite, PCPDTBT, and PCBM also facilitated the exciton dissociation in the multi-BHJ system between MAPbI3perovskite, PCPDTBT, and PCBM.
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- 2015
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22. Lymph Node Status Did Not Significantly Improve the Predictability of Survival in Patients With Clinically Early-Stage Endometrial Cancer.
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Bae, Hyo Sook, Lee, Jong-Min, Lee, Jae-Kwan, Kim, Jae-Weon, Cho, Chi-Heum, Kim, Seok-Mo, Park, Sang-Yoon, Park, Chan-Yong, Kim, Ki-Tae, and Kang, Sokbom
- Abstract
The aim of this study was to determine whether knowledge of lymph node status improves survival prediction in clinically early-stage endometrial cancer.The records of 661 patients with apparently uterine-confined disease were reviewed. The performance in predicting overall survival and cause-specific survival was compared between a multivariate prognostic model with nodal status and a model without nodal status by calculating Harrell concordance index.Among 661 patients with clinically early-stage endometrial cancer, the lymph node metastasis rate was 8.3% (55/661). Lymph node metastasis independently associated with cause-specific survival only when no stratification according to adjuvant treatment was applied (P = 0.035). After stratification according to adjuvant radiotherapy, lymph node status marginally associated with cause-specific survival (P = 0.073), whereas myometrial invasion retained its strong association with cause-specific survival (P < 0.001). However, there was no significant difference in the performance of the survival model using only uterine factors and the model using lymph node status and uterine factors (concordance index, 0.77 vs 0.77, respectively; P = 0.798).Knowledge of lymph node status did not significantly improve the performance of survival prediction in apparently uterine-confined endometrial cancer, although it was independently associated with survival. In the patients with clinically early-stage endometrial cancer, the accuracy of the prediction of survival was comparable between risk grouping without lymph node status and that including lymph node status. [ABSTRACT FROM AUTHOR]
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- 2014
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23. High-Performance Organic Solar Cells with EfficientSemiconducting Small Molecules Containing an Electron-Rich BenzodithiopheneDerivative.
- Author
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Lim, Namwoo, Cho, Nara, Paek, Sanghyun, Kim, Chulwoo, Lee, Jae Kwan, and Ko, Jaejung
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- 2014
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24. A Web-Based Nomogram Predicting Para-aortic Nodal Metastasis in Incompletely Staged Patients With Endometrial Cancer.
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Kang, Sokbom, Lee, Jong-Min, Lee, Jae-Kwan, Kim, Jae-Weon, Cho, Chi-Heum, Kim, Seok-Mo, Park, Sang-Yoon, Park, Chan-Yong, and Kim, Ki-Tae
- Abstract
The purpose of this study is to develop a Web-based nomogram for predicting the individualized risk of para-aortic nodal metastasis in incompletely staged patients with endometrial cancer.From 8 institutions, the medical records of 397 patients who underwent pelvic and para-aortic lymphadenectomy as a surgical staging procedure were retrospectively reviewed. A multivariate logistic regression model was created and internally validated by rigorous bootstrap resampling methods. Finally, the model was transformed into a user-friendly Web-based nomogram (http://www.kgog.org/nomogram/empa001.html).The rate of para-aortic nodal metastasis was 14.4% (57/397 patients). Using a stepwise variable selection, 4 variables including deep myometrial invasion, non-endometrioid subtype, lymphovascular space invasion, and log-transformed CA-125 levels were finally adopted. After 1000 repetitions of bootstrapping, all of these 4 variables retained a significant association with para-aortic nodal metastasis in the multivariate analysis—deep myometrial invasion (P = 0.001), non-endometrioid histologic subtype (P = 0.034), lymphovascular space invasion (P = 0.003), and log-transformed serum CA-125 levels (P = 0.004). The model showed good discrimination (C statistics = 0.87; 95% confidence interval, 0.82-0.92) and accurate calibration (Hosmer-Lemeshow P = 0.74).This nomogram showed good performance in predicting para-aortic metastasis in patients with endometrial cancer. The tool may be useful in determining the extent of lymphadenectomy after incomplete surgery. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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25. Association between Obesity and Cervical Microflora Dominated by Lactobacillus inersin Korean Women
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Oh, Hea Young, Seo, Sang-Soo, Kong, Ji-Sook, Lee, Jae-Kwan, and Kim, Mi Kyung
- Abstract
ABSTRACTLactobacillusspp. are associated with the maintenance of reproductive health, but obesity reduces fertility and is a risk factor for obstetric and neonatal complications. We assessed the association between obesity and the cervical Lactobacilluscomposition, which has not been examined previously. Pyrosequencing was performed using cervical swabs collected from 76 normal participants with negative results for cervical intraepithelial neoplasia (CIN) and 57 participants with CIN, based on histological examinations. Cluster analysis of nine Lactobacillusspp. was performed, and five cluster types were identified. The association between obesity and the Lactobacilluscommunity was assessed by logistic regression analysis after adjustment for confounding factors. The proportion of Lactobacillus inersincreased and that of Lactobacillus crispatusdecreased according to body mass index (BMI) categories, i.e., underweight (BMI of <18.5 kg m-2), normal weight (BMI of 18.5 to 22.9 kg m-2), overweight (BMI of 23.0 to 24.9 kg m-2), and obese (BMI of =25 kg m-2). The L. iners-dominant type had a significant association with obesity (odds ratio [OR], 7.55 [95% confidence interval [CI], 1.18 to 48.2]), compared to the L. crispatus-dominant type. The group with high values for the ratio obtained by dividing the relative abundance of L. inersby that of L. crispatushad a significant association with obesity (OR, 6.54 [95% CI, 1.22 to 35.1]), compared to the low-ratio group. Associations between obesity and the L. iners/L. crispatusratio were observed among young women (OR, 6.26 [95% CI, 1.15 to 33.9]) but not older women and in the normal group (OR, 6.97 [95% CI, 1.20 to 70.4]) but not the CIN group. Obesity was associated with cervical microflora dominated by L. inersin reproductive-age women without dysplasia.
- Published
- 2015
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26. Analyzing impairment of water bodies considering watershed characteristics in Geum River basin in Korea
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Park, Jun Dae, Park, Jae Hong, Lee, Jae Kwan, and Oh, Seung Young
- Abstract
Determining impairment of water bodies in the light of watershed characteristics is useful in selecting applicable water quality indicators as target pollutants for the watershed management. This study investigated impaired water bodies using frequency analysis on a stream zone basis in Geum river basin in Korea. The river basin was divided into three stream zones by its characteristics. Selected water quality parameters discussed in this study were pH, DO, BOD, COD, SS, T-P, T-Coli, and F-Coli. Excess ratios of the water quality parameters were used to discriminate water bodies that did not meet water quality standards. Excess levels were used to classify the water quality degradation. Contributions of the tributaries were also estimated to identify the major contributors to the stream zones. The upstream zone showed that the indicators for impaired water bodies were BOD, COD, T-P, and T-Coli which indicated especially high excess level. The downstream zone revealed that COD, T-P, and T-Coli were the indicators and the M/D stream zone revealed that the indicators were BOD, COD, and T-P. The major contributors in the upstream are Okcheon, Juwon, Bonghwang, and Pumgok tributaries. In the downstream, Nonsan, Seokseong, Gab, Yudeung, and Daejeon were the major contributing tributaries and in the M/D stream zone, the major contributors were Iksan, Gobu, and Jeongeup tributaries.
- Published
- 2015
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27. Analyzing impairment of water bodies considering watershed characteristics in Geum River basin in Korea
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Park, Jun Dae, Park, Jae Hong, Lee, Jae Kwan, and Oh, Seung Young
- Abstract
AbstractDetermining impairment of water bodies in the light of watershed characteristics is useful in selecting applicable water quality indicators as target pollutants for the watershed management. This study investigated impaired water bodies using frequency analysis on a stream zone basis in Geum river basin in Korea. The river basin was divided into three stream zones by its characteristics. Selected water quality parameters discussed in this study were pH, DO, BOD, COD, SS, T-P, T-Coli, and F-Coli. Excess ratios of the water quality parameters were used to discriminate water bodies that did not meet water quality standards. Excess levels were used to classify the water quality degradation. Contributions of the tributaries were also estimated to identify the major contributors to the stream zones. The upstream zone showed that the indicators for impaired water bodies were BOD, COD, T-P, and T-Coli which indicated especially high excess level. The downstream zone revealed that COD, T-P, and T-Coli were the indicators and the M/D stream zone revealed that the indicators were BOD, COD, and T-P. The major contributors in the upstream are Okcheon, Juwon, Bonghwang, and Pumgok tributaries. In the downstream, Nonsan, Seokseong, Gab, Yudeung, and Daejeon were the major contributing tributaries and in the M/D stream zone, the major contributors were Iksan, Gobu, and Jeongeup tributaries.
- Published
- 2015
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28. Assessment of cervical cancer screening policy in Korea for women over age 65.
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Lee, Sanghoon, Park, Hyun Tae, Hong, Jin Hwa, Song, Jae Yun, Lee, Jae Kwan, Lee, Nak Woo, Kim, Tak, and Lee, Kyu Wan
- Abstract
Abstract: Objectives: The purpose of this study was to evaluate the efficacy of cervical cancer screening (CCS) in Korean women over 65years of age and to determine the age at which CCS becomes inefficacious, so that providers and policy makers can be more informed about when to stop screening. Materials and Methods: The data of 999 women, who underwent CCS followed by colposcopy at the Korea University hospital from January 2007 to May 2012, were retrospectively evaluated. Age groups were classified as <21 (n=11), 21–29 (n=128), 30–65 (n=768), and >65 (n=92). To evaluate the value of CCS in women older than 65, age groups were simply divided into ≤65 or >65. Participants were also categorized into five strata of age groups (≤55 vs. >55, ≤60 vs. >60, ≤65 vs. >65, ≤70 vs. >70, and ≤75 vs. >75) that were compared to the sample to assess a cutoff-age at which to cease screening based on decreased efficacy. Results: The mean age was 45.5±13.7 (range14–80). There were no differences in the pathologic results for cervical intraepithelial neoplasia (CIN) values of
65 (13.0%) vs. ≤65 (6.6%), (p=0.037). To assess the cervical cancer incidence (CCI) from the larger number of cases by age, a prediction formula was calculated from a national dataset. CCI (per 100,000) continuously increased by age even for those in their 90s. Conclusion: CCS is still necessary in Korean women older than 65 since there is a substantial CCI rate occurring in these women. It is time to consider new guidelines that include the appropriate age and conditions for discontinuing screening. [Copyright &y& Elsevier] - Published
- 2013
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29. Detection of MicroRNA as Novel Biomarkers of Epithelial Ovarian Cancer From the Serum of Ovarian Cancer Patient.
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Chung, Ye-Won, Bae, Hyo-Sook, Song, Jae-Yun, Lee, Jae Kwan, Lee, Nak Woo, Kim, Tak, and Lee, Kyu-wan
- Abstract
MicroRNA (miRNA) is an abundant class of small noncoding RNAs that act as gene regulators. Recent studies have suggested that miRNA deregulation is associated with the initiation and progression of human cancer. However, information about cancer-related miRNA is mostly limited to tissue miRNA. The aim of this study was to find specific profiles of serum-derived miRNAs of ovarian cancer based on a comparative study using a miRNA microarray of serum, tissue, and ascites.From 2 ovarian cancer patients and a healthy control, total RNA was isolated from their serum, tissue, and ascites, respectively, and analyzed by a microarray. Under the comparative study of each miRNA microarray, we sorted out several miRNAs showing a consistent regulation tendency throughout all 3 specimens and the greatest range of alteration in serum as potential biomarkers. The availability of biomarkers was confirmed by qRT-PCR of 18 patients and 12 controls.Out of 2222 kinds of total miRNAs that were identified in the microarray analysis, 95 miRNAs were down-regulated and 88 miRNAs were up-regulated, in the serum, tissue, and ascites of cancer patients. Among the miRNAs that showed a consistent regulation tendency through all specimens and showed more than a 2-fold difference in serum, 5 miRNAs (miR-132, miR-26a, let-7b, miR-145, and miR-143) were determined as the 5 most markedly down-regulated miRNAs in the serum from ovarian cancer patients with respect to those of controls. Four miRNAs (miR-132, miR-26a, let-7b, and miR-145) out of 5 selected miRNAs were significantly underexpressed in the serum of ovarian cancer patients in qRT-PCR.Serum miR-132, miR-26a, let-7b, and miR-145 could be considered as potential candidates as novel biomarkers in serous ovarian cancer. Also, serum miRNAs is a promising and useful tool for discriminating between controls and patients with serous ovarian cancer. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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30. Planar Star-Shaped Organic Semiconductor with Fused Triphenylamine Core for Solution-Processed Small-Molecule Organic Solar Cells and Field-Effect Transistors.
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Paek, Sanghyun, Cho, Nara, Cho, Shinuk, Lee, Jae Kwan, and Ko, Jaejung
- Published
- 2012
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31. A multicenter, randomized, double-blind, placebo-controlled trial evaluating the efficacy and safety of a far infrared-emitting sericite belt in patients with primary dysmenorrhea.
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Lee, Chae Hyeong, Roh, Ju-Won, Lim, Chi-Yeon, Hong, Jin Hwa, Lee, Jae Kwan, and Min, Eung Gi
- Abstract
Summary: Objective: To determine the efficacy and safety of a sericite which emits far infrared rays in the management of primary dysmenorrhea. Design: A multicenter randomized double-blind placebo-controlled trial. Setting: One hundred four patients with primary dysmenorrhea were randomized to wear a sericite or placebo belt during sleep for 3 menstrual cycles, and followed for 2 menstrual cycles. Hot packs were used to heat the ceramics and ensure slight pain relief in both groups. Main outcome measures: The main outcome measures were the severity of dysmenorrhea assessed by a 10-point visual analog scale (VAS) and the number of patients who took analgesics at each menstrual cycle. Safety was evaluated by physical examination and self-reporting of adverse events. Results: The baseline VAS scores were 7.27±0.19 in the experimental group and 7.38±0.19 in the control group. The severity of dysmenorrhea gradually decreased during the treatment period in both groups, with major improvements observed in the third treatment cycle (4.96±0.30 in the experimental group and 5.69±0.30 in the control group, p =0.087). During the follow-up period, the decreased VAS score was maintained in the experimental group, whereas the VAS score gradually returned to baseline in the control group, which resulted in significant difference between the groups (5.08±0.31 vs. 6.47±0.31 at cycle 5, difference −1.39 [95% CI, −2.25 to −0.53], p =0.0017). The number of patients who took analgesics decreased in both groups, but the differences were not statistically significant. No serious adverse events related to wearing the sericite belt occurred. Conclusions: Our data suggest that a far infrared-emitting sericite belt with a hot pack might be used as an effective and safe non-pharmacologic treatment option for women with primary dysmenorrhea, with a prolonged effect after treatment. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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32. Association Between Serum Cytokine Profiles and Clearance or Persistence of High-Risk Human Papillomavirus Infection.
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Hong, Jin Hwa, Kim, Mi Kyung, Lee, In Ho, Kim, Tae Jin, Kwak, San Ha, Song, Seung Hun, and Lee, Jae Kwan
- Abstract
The cytokines that may be associated with the clearance or persistence of high-risk human papillomavirus (HPV) infection in patients with mild dysplasia or less of the uterine cervix were determined.A prospective study of 160 patients who harbored high-risk HPV with histologically confirmed mild dysplasia or less between January 2006 and August 2008 was performed. All patients were followed up at every 4 months during the first year. Human papillomavirus DNA tests by the Hybrid Capture 2 method (Digene, Gaithersburg, Md) were performed at every visit on all patients. Blood sampling was performed in all patients at their initial visit, and an enzyme-linked immunosorbent assay was used to measure the serum levels of interferon γ, tumor necrosis factor α, and interleukins 6 and 10.Of the 160 patients, 107 (66.9%) had clearance of HPV infection after 12 months of follow-up. The median age of patients in the persistence group was significantly higher than that of patients in the clearance group (47 vs 38 years, respectively; range, 21-77 vs 21-71 years, respectively; P = 0.0009). In univariate analysis, the number of patients with serum negative for tumor necrosis factor α was significantly higher in the clearance group than the persistence group (P = 0.0363). However, in multivariate logistic regression analysis, all 4 cytokines failed to show any significant association with the clearance or persistence of HPV infection.The baseline serum cytokine levels were not associated with the clearance or persistence of HPV infection. Systemic immunity may not influence the natural history of HPV infection. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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33. Dietary Supplements Reduce the Risk of Cervical Intraepithelial Neoplasia.
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Hwang, Jong Ha, Kim, Mi Kyung, and Lee, Jae Kwan
- Abstract
To examine the effects of dietary supplements on high-risk human papillomavirus (HPV) infection and cervical carcinogenesis.A multi-institutional cross-sectional study was carried out to examine whether dietary supplements were associated with the risk of cervical intraepithelial neoplasia (CIN). We enrolled 1096 women aged 18 to 65 years to participate in an HPV cohort study from March 2006 up to present. For this analysis, we included 328 HPV-positive women (166 controls; 90 CIN I and 72 CIN II/III). The details of each participant's routine dietary intake during the prior year were collected. Specific dietary supplements were classified into 5 categories, namely, multivitamins, multinutrients, vitamin C, calcium, and miscellaneous.A higher HPV viral load was associated with an increased risk of CIN II/III (odds ratio [OR], 3.32; 95% confidence interval [CI], 1.54-7.16; P for trend 0.002). Dietary supplement use including multivitamins (OR, 0.21; 95% CI, 0.09-0.48), vitamins A (OR, 0.19; 95% CI, 0.07-0.53), C (OR, 0.24; 95% CI, 0.10-0.56), E (OR, 0.20; 95% CI, 0.07-0.53), and calcium (OR, 0.21; 95% CI, 0.08-0.50) was significantly associated with a lower risk of CIN II/III. The patients who took multivitamins and had a lower HPV viral load (<15.5 relative light units/positive control) had a significantly decreased frequency of CIN I (OR, 0.35; 95% CI, 0.14-0.87; interaction P = 0.925) and CIN II/III (OR, 0.11; 95% CI, 0.04-0.37; interaction P = 0.304).The findings of this study suggest that dietary supplements may reduce the risk of CINs in women with high-risk HPV infection. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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34. Comparison of Human Papillomavirus Detection and Typing by Hybrid Capture 2, Linear Array, DNA Chip, and Cycle Sequencing in Cervical Swab Samples.
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Lee, Jae Kwan, Kim, Mi Kyung, Song, Seung Hun, Hong, Jin Hwa, Min, Kyung Jin, Kim, Jae Hoon, Song, Eun-Seop, Lee, Jungpil, Lee, Jong-Min, and Hur, Soo-Young
- Abstract
Although the Hybrid Capture II (HC II) assay can detect 13 high-risk human papillomavirus (HPVs), it does not yield any genotype-specific information. We evaluated the performance of 4 HPV DNA tests, namely, HC II, Linear Array (LA), DNA chip, and cycle sequencing for their capacity to detect the presence of high-risk HPV DNA and HPV-associated cervical lesions. Seventy-six women who were referred to the colposcopy clinic for abnormal cytology were enrolled. The women were examined using liquid-based cytology, colposcopy-directed biopsy, and HPV DNA tests. After DNA extraction from a single sample, HPV DNA tests were performed by all 4 methods on the same specimen. The LA test has higher HPV-positive rates than HC II for cervical intraepithelial neoplasia I (83.3% vs 61.1%; P < 0.01) and for cervical intraepithelial neoplasia II and more severe lesions (100.0% vs 80.0%; P < 0.01). The concordance between the DNA chip and LA tests was 89.5%, confirming substantial agreement (κ coefficient = 0.73), and the concordance between HC II and the DNA chip was 80.3%, also showing substantial agreement (κ coefficient = 0.738). The concordance for 15 high-risk HPV genotypes between LA and sequencing was 82.5% with a κ value of 0.536. Furthermore, the LA test was more sensitive in the detection of high-grade cervical lesions than HC II (100% vs 92.3%, P < 0.01). The LA test showed superior sensitivity in the detection of clinically relevant HPV infections and has proven to be an accurate tool for identifying individual HPV types, especially in cases of multiple HPV infections. [ABSTRACT FROM AUTHOR]
- Published
- 2009
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35. Laparoscopic Management of Early Omental Pregnancy Detected by Magnetic Resonance Imaging.
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Yi, Kyong Wook, Yeo, Min Koo, Shin, Jung-Ho, Kim, Kyeong Ah, Oh, Min-Jeong, Lee, Jae Kwan, Hur, Jun-Young, and Saw, Ho-Suk
- Abstract
Abstract: Omental pregnancy is a very rare form of abdominal pregnancy, and can be life threatening in cases of rupture, which may lead to massive hemorrhage. A 28-year-old woman in the ninth week of amenorrhea had severe abdominal pain, and showed unstable vital signs caused by intraperitoneal hemorrhage. Emergency laparoscopy revealed large amounts of blood and blood clots in abdominal cavity, and active bleeding was observed from the right ovary. Uterus, left ovary, and bilateral fallopian tubes were grossly free. However, serum β-human chorionic gonadotropin level increased after the operation, and histopathologic results did not show evidence of pregnancy in either right ovary or endometrium. Abdominal magnetic resonance imaging was performed and revealed a well-defined cystic mass at the lateral side of the ascending colon, suggested to be the focus of ectopic pregnancy. The patient was approached laparoscopically and consequently underwent partial omentectomy. [Copyright &y& Elsevier]
- Published
- 2008
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36. Predictors of leiomyoma recurrence after laparoscopic myomectomy.
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Yoo, Eun-Hee, Lee, Paul I., Huh, Chu-Yeop, Kim, Dong-Ho, Lee, Byung-Seok, Lee, Jae-Kwan, and Kim, Donguk
- Subjects
SMOOTH muscle surgery ,CANCER relapse ,ULTRASONIC imaging ,HEMATOCRIT - Abstract
Abstract: Study objective: To evaluate recurrence and reoperation rate after laparoscopic myomectomy in relation to risk factors and identify suitable candidates for laparoscopic myomectomy to decrease recurrence. Design: Multicenter retrospective cohort study (Canadian Task Force classification II-2). Setting: Five university hospitals and a university-affiliated teaching hospital. Patients: Five hundred and twelve women who underwent laparoscopic myomectomy between 1995 and 2004. All patients had a follow-up with clinical examination and transvaginal sonography for a median 13 months after surgery. Intervention: Laparoscopic myomectomy. Measurements and main results: Recurrence was defined as the appearance of a leiomyoma on ultrasound examination or identification of leiomyoma during subsequent surgery after the initial surgery. Cox regression (full model) analysis of the possible risk factors for recurrence followed by a stepwise variable selection was performed to eliminate confounding factors. The cumulative probability of leiomyoma recurrence increased steadily during the follow-up period, 11.7% after 1 year, 36.1% after 3 years, 52.9% at 5 years, and reached 84.4% at 8 years. The cumulative probability of reoperation for recurrent leiomyoma was much lower: 6.7% at 5 years and 16% at 8 years. Significant risk factors that were independently associated with cumulative recurrence were age, preoperative number of myoma, preoperative uterine size by pelvic examination, presence of associated pelvic disease, and delivery after laparoscopic myomectomy. The operative time and change of hematocrit were associated with the reoperation. Those who had fewer than 2 myomas before surgery, uterus size less than 13 gestational weeks measured by pelvic examination, no childbirth after laparoscopic myomectomy, and age at index surgery less than 35.5 years showed the lowest recurrence after laparoscopic myomectomy from Classification and Regression trees analysis. Conclusion: The risk of recurrence of leiomyoma after laparoscopic myomectomy is linked with the age, preoperative number of leiomyoma, preoperative uterine size, presence of associated pelvic disease, and childbirth after surgery. [Copyright &y& Elsevier]
- Published
- 2007
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37. Septate uterus with double cervices, unilaterally obstructed vaginal septum, and ipsilateral renal agenesis: A rare combination of müllerian and wolffian anomalies complicated by severe endometriosis in an adolescent.
- Author
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Hur, Jun-Young, Shin, Jung-Ho, Lee, Jae-Kwan, Oh, Min-Jeong, Saw, Ho-Suk, Park, Yong-Kyun, and Lee, Kyu Wan
- Subjects
UTERINE diseases ,WOLFFIAN body ,CERVIX uteri ,POTTER'S syndrome ,VAGINA - Abstract
Abstract: We present a previously unreported combination of müllerian and wolffian anomalies of a septate uterus with double cervices, unilaterally obstructed vaginal septum, and ipsilateral renal agenesis; this constellation of findings may offer clues that could modify classic embryologic explanations. In spite of the young age of our patient (15-years old), a chief complaint of malodorous vaginal discharge, and absence of dysmenorrhea or any other symptoms of endometriosis, laparoscopic examination revealed severe endometriosis with dense adhesions, probably as a result of abundant menstrual regurgitation. Laparoscopic resection of endometriotic lesions, adhesiolysis, and vaginoscopic septotomy were successfully performed while preserving hymenal integrity. [Copyright &y& Elsevier]
- Published
- 2007
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38. Natural History of Low-Grade Squamous Intraepithelial Lesion.
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Saw, Ho–Suk, Lee, Jae–Kwan, Lee, Hwa–Lynn, Jee, Hyun–Jun, and Hyun, Jong–Jin
- Subjects
NATURAL history ,EPITHELIAL cells ,PAP test ,DISEASES - Abstract
Abstract. [ABSTRACT FROM AUTHOR]
- Published
- 2001
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39. Efficient Organic Solar Cells with Star-Shaped Small Molecules Comprising of Planar Donating Core and Accepting Edges
- Author
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Paek, Sanghyun, Choi, Hyeju, Sim, Jangkeun, Song, Kihyoung, Lee, Jae Kwan, and Ko, Jaejung
- Abstract
High efficiency small molecules were synthesized and characterized in solution-processed organic solar cells. These were star-shaped comprising of fused triphenylamine donating core, hexyl cyano acetate or methylene malononitrile accepting edges, and alkyl-substituted therthiophene bridge unit, exhibiting a noteworthy power conversion efficiency of 4.18% in solar cell device from bulk-heterojunction films with PC71BM.
- Published
- 2014
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40. Improved External Quantum Efficiency from Solution-Processed (CH3NH3)PbI3Perovskite/PC71BM Planar Heterojunction for High Efficiency Hybrid Solar Cells
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Paek, Sanghyun, Cho, Nara, Choi, Hyeju, Jeong, Hanbin, Lim, Jin Sung, Hwang, Jun-Yeon, Lee, Jae Kwan, and Ko, Jaejung
- Abstract
Well-organized (CH3NH3)PbI3perovskite films fabricated from various solution-processing conditions were characterized and used in hybrid solar cells with PC71BM planar heterojuncion films, exhibiting a high power-conversion efficiency of 12.2% with the better photocurrent and fill factor compared to those with PC61BM due to a better spectral response in the visible region and a better planar junction with the Ag electrode than the PC61BM.
- Published
- 2014
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41. Effect of TiO2 Nanotube Arrays on Osseointegration for Dental Implant
- Author
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Shim, Seong Cheol, Choe, Byung Hak, Jang, In San, Choi, Dong Soon, Lee, Jae Kwan, Cha, Bong Kuen, and Choi, Won Youl
- Abstract
Dental implant with porous surface has been studied by many researchers because it has a good osseointegration. TiO
2 nanotube arrays on the surface of dental implant could provide the porous surface, the space of drug loading, and better biocompatibility than bare surface. To investigate the effects of TiO2 nanotube arrays for dental implant, commercial dental implant was used and its surface was anodized to fabricate TiO2 nanotube arrays. Highly ordered TiO2 nanotube arrays were grown on the surface of dental implant. Ethylene glycol based electrolyte was used in anodic oxidation process. Anodic oxidation voltage and time were 50V and 15 min, respectively. The micro structure was observed by FE-SEM. The diameter and length of TiO2 nanotube arrays were ~60 nm and 10μm, respectively. BMP-2 was loaded in TiO2 nanotube arrays. For in-vivo testing, the dental implant with TiO2 nanotube arrays was applied to rabbit legs for 8 weeks. In dental implant with TiO2 nanotube arrays, good osseointegration properties were observed. TiO2 nanotube arrays fabricated by anodic oxidation will be very useful in the field of bone implants and bone tissue engineering.- Published
- 2014
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42. Circulating endothelial progenitor cells in gynaecological cancer
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Kim, Yoon Byoung, Chung, Ye Won, Bae, Hyo Sook, Lee, Jae Kwan, Lee, Nak Woo, Lee, Kyu Wan, and Song, Jae Yun
- Abstract
Objectives To compare the frequency and absolute numbers of circulating endothelial progenitor cells (EPCs) in healthy control subjects and patients with gynaecological cancer, and to test the hypothesis that cancer treatment lowers EPC numbers.Methods Patients with cervical or ovarian cancer and healthy control subjects provided peripheral blood samples for the isolation of mononuclear cells. EPCs were identified by quadruple immunofluorescence staining and flow cytometry as CD45–/CD34+/CD133+/vascular endothelial growth factor receptor 2 (VEGFR2)+cells.Results In total, 28 participants were enrolled. Circulating EPCs were present at higher frequencies (and in greater absolute numbers) in patients with cervical or ovarian cancer (n= 14) than in controls (n= 14). Concurrent chemoradiation therapy or surgery significantly reduced the frequency and number of EPCs in patients with gynaecological cancer, compared with pretreatment levels.Conclusions EPC levels decline throughout cancer treatment; their measurement may therefore be a useful surrogate marker to monitor treatment response.
- Published
- 2013
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43. Efficient Organic Semiconductors Containing Fluorine-Substituted Benzothiadiazole for Solution-Processed Small Molecule Organic Solar Cells
- Author
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Paek, Sanghyun, Cho, Nara, Song, Kihyung, Jun, Moo-Jin, Lee, Jae Kwan, and Ko, Jaejung
- Abstract
The synthesis and photovoltaic characteristics of new organic semiconductors, [bisDMFA-Th]-BT-HxTh3(1), [bisDMFA-Th]-MonoF-BT-HxTh3(2), and [bisDMFA-Th]-DiF-BT-HxTh3(3), are reported. These semiconductors have an unsymmetrical donor A–acceptor–donor B (DA-A-DB) framework composed of a benzothiadiazole (BT; fluorinated or unfluorinated) acceptor between two different donors, bisDMFA and terthiophene. We have demonstrated that these compounds are suitable for use in p-type organic semiconductors for high efficiency solution-processed small molecule organic solar cells (SMOSCs), for which we achieved a remarkable power conversion efficiency of 4.24% with a maximum Vocof 0.89 V. The fluorine substitution on BT decreased its HOMO level and increased hole mobilities of [bisDMFA-Th]-BT-HxTh3derivatives, leading to an increased Vocin the SMOSCs and improved hole carrier transport properties of the material. In addition, we determined that the insertion of a TiOxfunctional layer into a bulk heterojunction (BHJ) solar cell significantly reduced the interfacial resistance between the photoactive film and metal electrode, resulting in an increased photocurrent with facile electron transfer between these two layers.
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- 2012
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44. Visible light-induced photocurable (forming a film) low molecular weight chitosan derivatives for biomedical applications: Synthesis, characterization and in vitro biocompatibility.
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Seo, Si Yoong, Park, Shin Hye, Lee, Hyung Jae, Na, Ha Na, Kim, Kwang Il, Han, Dong Keun, Lee, Jae Kwan, Ito, Yoshihiro, and Son, Tae Il
- Subjects
ORGANIC synthesis ,MOLECULAR weights ,CHITOSAN ,CHEMICAL derivatives ,BIOCOMPATIBILITY ,NITROUS acid ,CHEMICAL decomposition ,ETHERS ,NUCLEAR magnetic resonance spectroscopy - Abstract
Abstract: In this work, visible light-induced photocurable low molecular weight chitosan derivatives (F-LMC: furfuryl-low molecular weight chitosan) were prepared by nitrous acid decomposition followed by modification with furfuryl glycidyl ether. The structure of the decomposition products (LMC: low molecular weight chitosan) was characterized by FT-IR analysis. The structure and molecular weight of F-LMC, prepared from the decomposition products by modification with furfuryl glycidyl ether, were characterized by
1 H NMR and GPC analysis, respectively. F-LMC was initially water-soluble, but after the exposure to visible light, it became water-insoluble, indicating that F-LMC undergoes visible light-induced inter- and intra-molecular cross-linking. The cross-linking was determined by concentration of F-LMC and time of exposure to visible light. The cytotoxicity test using fibroblast 3T3 showed no toxicity of F-LMC. Cross-linked F-LMC surface inhibited cell adherence. [Copyright &y& Elsevier]- Published
- 2012
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45. Primary hepatoid adenocarcinoma of the endometrium with a high alphafetoprotein level
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Hwang, Jong Ha, Song, Seung Hun, Kim, Young Hye, Shin, Bong Kyung, Lee, Jae Kwan, Lee, Nak Woo, and Lee, Kyu Wan
- Abstract
Primary hepatoid adenocarcinoma of the endometrium is a rare tumour that is similar to hepatocellular carcinoma histologically. Here, a patient with primary hepatoid carcinoma of the endometrium with a high alphafetoprotein (AFP) level (90,508 ng/mL) is presented in a 75-year-old woman. The pelvic computed tomography and magnetic resonance imaging suggested a submucosal leiomyoma with degeneration or endometrial hyperplasia. However, the endometrial biopsy revealed a primary hepatoid carcinoma of the endometrium. The patient underwent a staging laparotomy including a total abdominal hysterectomy, bilateral salpingo-oophorectomy and lymph node sampling with pelvic cytology. The AFP level can be highly elevated in hepatoid carcinoma of the endometrium.
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- 2011
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46. Current status of Korean streams and exploring areas with high necessity for stream structure restoration
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Jeong, Kwang-Seuk, Kim, Han-Gook, Jeong, Jong-Chul, Kim, Dong-Kyun, Kim, Hyun-Woo, Lee, Jae-Kwan, Oh, Jong-Min, and Joo, Gea-Jae
- Abstract
Stream restoration is an important process affecting the ecological health of stream ecosystems. There have been numerous cases of restoration, dealing with either structural or biological changes. In Korea, most restoration projects have merely dealt with improving hydrological characteristics or water quality; however, in recent years the improvement of ecological characteristics has been an increasing focus for restoration projects. In this study, we utilized data collected from 5675 stream sites in May 2007 to discover general patterns of anthropogenic modification in Korean streams. The survey results after application of the stream modification index (SMI; presence or absence type; high scores indicate more disturbed) provided a general distribution of disturbed/undisturbed streams or rivers in the watershed. We then compared the level of modification with the socio-geographical patterns (population, land coverage, elevation, and slope) for the watershed. The results show that streams in highly populated areas suffered from human modification compared with other well-preserved stream sites. In metropolitan cities, urbanized areas had positive relationship as identified by a high SMI. On the other hand, agricultural land cover identified an SMI increase for lowland river area. In general, mountainous streams possessed a better status in stream morphology due to different land-cover patterns (i.e., mainly forested area); however, some mountainous areas were impacted by concentrated summer rainfall. We could distinguish the forcing variables (i.e., land use pattern) for the disturbed streams through a comparison between the SMI and geographical information; the SMI application was able to identify areas of high necessity for restoration.
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- 2011
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47. Overview and application of the National Aquatic Ecological Monitoring Program (NAEMP) in Korea
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Lee, Sang-Woo, Hwang, Soon-Jin, Lee, Jae-Kwan, Jung, Dong-Il, Park, Yeon-Jae, and Kim, Ji-Tae
- Abstract
This paper provides an overview of the development and application of the National Aquatic Ecological Monitoring Program (NAEMP) in Korea, which uses biological and habitat–riparian criteria for river/stream and watershed management. Development of NAEMP began in 2003, with recognition by the Korean Ministry of Environment (MOE) of the limitations of applying chemical parameters (e.g., biochemical oxygen demand (BOD)) as the principal targets of water environment management. Ecosystem health criteria under NAEMP were developed from 2003 to 2006. Candidate sites for monitoring were also screened and established across the country. NAEMP was implemented in 2007, and since then a standard protocol of nationwide monitoring based on multi-criteria has been implemented to assess the ecological condition of rivers and streams. The monitoring results indicate that many Korean rivers and streams are severely degraded, with biological conditions that are much worse than their water chemistry suggests. In 2009, 24% of rivers and streams were in classes C (Fair) and D (Poor) for BOD, but more than 71, 53, and 27% were categorized as Fair to Poor according to fish, diatom, and benthic macroinvertebrate assemblages, respectively. NAEMP is promising in that the results have already had great impacts on policy making and scientific research relevant to lotic water environment and watershed management in Korea. In the future, NAEMP results will be used to develop more aggressive regulations for the preservation and restoration of rivers/streams, riparian buffer areas and watersheds. Another future aim of the NAEMP is to develop aquatic ecological modeling based on the monitoring results.
- Published
- 2011
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48. Bone mineral density after concurrent chemoradiation in patients with uterine cervical cancer
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Hwang, Jong Ha, Song, Seung Hun, Lee, Jae Kwan, Lee, Nak Woo, and Lee, Kyu Wan
- Abstract
The aim of this study was to examine the bone mineral density (BMD) of women with cervical cancer treated with chemoradiation and to compare the outcomes with those of women with myomas treated by hysterectomy.
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- 2010
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49. Borderline-like Mucinous Tumor Arising in Mature Cystic Teratoma of the Ovary Associated With Pseudomyxoma Peritonei
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Hwang, Jong Ha, So, Kyeong-A, Modi, Gayatri, Lee, Jae Kwan, Lee, Nak Woo, Lee, Kyu Wan, and Kim, Insun
- Abstract
Pseudomyxoma peritonei (PMP) is now considered to originate from appendiceal mucinous neoplasms in almost all cases. However, a mucinous neoplasm arising in a mature cystic teratoma is rarely responsible for PMP. We herein report 2 cases of PMP associated with borderline mucinous tumors arising in mature cystic teratoma. Both ovarian borderline mucinous tumors were morphologically and immunohistochemically similar to the secondary tumors from appendiceal origin, and PMP was histologically similar to peritoneal adenomucinosis.
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- 2009
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50. Evaluation of Tensile Properties and Bending-Induced Residual Stress of Slender Copper Pipe
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Kim, Sang Young, Kim, Hyung Ick, Seok, Chang Sung, Lee, Jae Kwan, Mo, Jin Yong, and Park, Deug Yong
- Abstract
Used pipes in various mechanisms and structures are produced from raw material by extruding and drawing. The properties such as yield strength, tensile strength, and elongation of a pipe produced by these methods are different from the properties of their raw material. But designers use the properties of the raw material because the actual properties of the pipes are difficult to obtain from testing. Also, the pipe is used after it has been bent in a complex manner and cut to fit it to mechanisms and structures. The bending process, especially, induces deformation of the pipe’s section and residual stress, which are involved in the plastic deformation of the bended pipes. This residual stress affects the pipe’s properties, including its fatigue life. Therefore, it is very important to understand the residual stress of a material. But, the distribution of residual stress of a U-shaped pipe, which is examined in this study, is very complicated and cannot be measured exactly.
- Published
- 2006
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