139 results on '"Lin, Miao"'
Search Results
2. Plasma Proteomics to Identify Drug Targets and Potential Drugs for Retinal Artery Occlusion: An Integrated Analysis in the UK Biobank
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Cao, Jiahui, Zhuang, Minjing, Kong, Huiqian, Lai, Chunran, Su, Ting, Liang, Anyi, Wang, Zicheng, Wu, Qiaowei, Fang, Ying, Hu, Yijun, Zhang, Xiayin, Lin, Miao, and Yu, Honghua
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Retinal artery occlusion (RAO), which is positively correlated with acute ischemic stroke (IS) and results in severe visual impairment, lacks effective intervention drugs. This study aims to perform integrated analysis using UK Biobank plasma proteome data of RAO and IS to identify potential targets and preventive drugs. A total of 7191 participants (22 RAO patients, 1457 IS patients, 8 individuals with both RAO and IS, and 5704 healthy age-gender-matched controls) were included in this study. Unique 1461 protein expression profiles of RAO, IS, and the combined data set, extracted from UK Biobank Plasma proteomics projects, were analyzed using both differential expression analysis and elastic network regression (Enet) methods to identify shared key proteins. Subsequent analyses, including single cell type expression assessment, pathway enrichment, and druggability analysis, were conducted for verifying shared key proteins and discovery of new drugs. Five proteins were found to be shared among the samples, with all of them showing upregulation. Notably, adhesion G-protein coupled receptor G1 (ADGRG1) exhibited high expression in glial cells of the brain and eye tissues. Gene set enrichment analysis revealed pathways associated with lipid metabolism and vascular regulation and inflammation. Druggability analysis unveiled 15 drug candidates targeting ADGRG1, which demonstrated protective effects against RAO, especially troglitazone (−8.5 kcal/mol). Our study identified novel risk proteins and therapeutic drugs associated with the rare disease RAO, providing valuable insights into potential intervention strategies.
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- 2024
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3. Phonon Directionality Impacts Electron–Phonon Coupling and Polarization of the Band-Edge Emission in Two-Dimensional Metal Halide Perovskites
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Krahne, Roman, Schleusener, Alexander, Faraji, Mehrdad, Li, Lin-Han, Lin, Miao-Ling, and Tan, Ping-Heng
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Two-dimensional metal halide perovskites are highly versatile for light-driven applications due to their exceptional variety in material composition, which can be exploited for the tunability of mechanical and optoelectronic properties. The band-edge emission is defined by the structure and composition of both organic and inorganic layers, and electron–phonon coupling plays a crucial role in the recombination dynamics. However, the nature of the electron–phonon coupling and what kind of phonons are involved are still under debate. Here we investigate the emission, reflectance, and phonon response from single two-dimensional lead iodide microcrystals with angle-resolved polarized spectroscopy. We find an intricate dependence of the emission polarization with the vibrational directionality in the materials, which reveals that several bands of low-frequency phonons with nonorthogonal directionality contribute to the band-edge emission. Such complex electron–phonon coupling requires adequate models to predict the thermal broadening of the emission and provides opportunities to design polarization properties.
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- 2024
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4. Enhancing Ki-67 Prediction in Breast Cancer: Integrating Intratumoral and Peritumoral Radiomics From Automated Breast Ultrasound via Machine Learning.
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Li, Fang, Zhu, Tong-wei, Lin, Miao, Zhang, Xiao-ting, Zhang, Ya-li, Zhou, Ai-li, and Huang, De-yi
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Traditional Ki-67 evaluation in breast cancer (BC) via core needle biopsy is limited by repeatability and heterogeneity. The automated breast ultrasound system (ABUS) offers reproducibility but is constrained to morphological and echoic assessments. Radiomics and machine learning (ML) offer solutions, but their integration for improving Ki-67 predictive accuracy in BC remains unexplored. This study aims to enhance ABUS by integrating ML-assisted radiomics for Ki-67 prediction in BC, with a focus on both intratumoral and peritumoral regions. A retrospective analysis was conducted on 936 BC patients, split into training (n = 655) and testing (n = 281) cohorts. Radiomics features were extracted from intra- and peritumoral regions via ABUS. Feature selection involved Z-score normalization, intraclass correlation, Wilcoxon rank sum tests, minimum redundancy maximum relevance, and least absolute shrinkage and selection operator logistic regression. ML classifiers were trained and optimized for enhanced predictive accuracy. The interpretability of the optimized model was further augmented by employing Shapley additive explanations (SHAP). Of the 2632 radiomics features in each patient, 15 were significantly associated with Ki-67 levels. The support vector machine (SVM) was identified as the optimal classifier, with area under the receiver operating characteristic curve values of 0.868 (training) and 0.822 (testing). SHAP analysis indicated that five peritumoral and two intratumoral features, along with age and lymph node status, were key determinants in the predictive model. Integrating ML with ABUS-based radiomics effectively enhances Ki-67 prediction in BC, demonstrating the SVM model's strong performance with both radiomics and clinical factors. [ABSTRACT FROM AUTHOR]
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- 2024
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5. Subxiphoid versus lateral intercostal thoracoscopic thymectomy for suspected thymoma: Results of a randomized controlled trial.
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Wang, Shuai, Yang, Xinyu, Jiang, Jiahao, Lin, Miao, Fan, Hong, Zhang, Zhilong, Xia, Hongwei, Wang, Hao, Liang, Fei, Ding, Jianyong, and Tan, Lijie
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This trial was to evaluate the efficacy of subxiphoid approach thoracoscopic thymectomy for postoperative pain control and length of hospital stay compared with a lateral intercostal approach thoracoscopic thymectomy. This multicenter, open-label, randomized clinical superiority trial enrolled 101 eligible participants clinically diagnosed with Masaoka-Koga I-II thymoma between August 15, 2021, and February 15, 2022. Each enrolled participant was randomized and underwent subxiphoid approach thoracoscopic thymectomy or lateral intercostal approach thoracoscopic thymectomy. A per-protocol analysis for each coprimary outcome was performed in addition to the main intention-to-treat analysis. In the analysis for the coprimary outcomes, the pain Visual Analog Scale score area under the curve at 0 to 7 days was lower in the subxiphoid approach thoracoscopic thymectomy group than in the lateral intercostal approach thoracoscopic thymectomy group (difference, −4.82; 98.3% CI, −8.84 to −0.80). However, there was no significant difference between the 2 groups in the length of hospital stay (difference, 0.318; 98.3% CI, −0.190 to 0.825) or cumulative opioid consumption after surgery (difference, −4.630; 98.3% CI, −9.530 to 0.272). All patients underwent complete resection, and there was no significant difference (7.84% vs 8.00%, P = 1.000) in the rate of complications between the 2 groups. No recurrence or death occurred in the postoperative 6 months. This study found improved pain and similar length of hospital stay associated with the subxiphoid approach compared with the lateral intercostal approach in patients with suspected Masaoka-Koga I-II thymoma. [Display omitted] [ABSTRACT FROM AUTHOR]
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- 2024
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6. Prognostic value of tumor deposits and positive lymph node ratio in stage III colorectal cancer: a retrospective cohort study.
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Lei Liu, Jie Ji, Xianxiu Ge, Zuhong Ji, Jiacong Li, Jie Wu, Juntao Zhu, Jianan Yao, Fangyu Zhu, Boneng Mao, Zhihong Cao, Jinyi Zhou, Lin Miao, Guozhong Ji, and Dong Hang
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Background: In colorectal cancer (CRC), tumor deposits (TD) have been used to guide the N staging only in node-negative patients. It remains unknown about the prognostic value of TD in combination with positive lymph node ratio (LNR) in stage III CRC. Patients and methods: The authors analyzed data from 31 139 eligible patients diagnosed with stage III CRC, including 30 230 from the Surveillance, Epidemiology, and End Results (SEER) database as a training set and 909 from two Chinese hospitals as a validation set. The associations of TD and LNR with cancer-specific survival (CSS) and overall survival (OS) were evaluated using the Kaplan-Meier method and Cox regression models. Results: Both TD-positive and high LNR (value =0.4) were associated with worse CSS in the training [multivariable hazard ratio (HR), 1.50; 95% CI: 1.43-1.58 and HR, 1.74; 95% CI: 1.62-1.86, respectively] and validation sets (HR, 1.90; 95% CI: 1.41-2.54 and HR, 2.01; 95% CI: 1.29-3.15, respectively). Compared to patients with TD-negative and low LNR (value <0.4), those with TDpositive and high LNR had a 4.09-fold risk of CRC-specific death in the training set (HR, 4.09; 95% CI: 3.54-4.72) and 4.60-fold risk in the validation set (HR, 4.60; 95% CI: 2.88-7.35). Patients with TD-positive/H-LNR CRC on the right side had the worst prognosis (P <0.001). The combined variable of TD and LNR contributed the most to CSS prediction in the training (24.26%) and validation (32.31%) sets. A nomogram including TD and LNR showed satisfactory discriminative ability, and calibration curves indicated favorable consistency in both the training and validation sets. Conclusions: TD and LNR represent independent prognostic predictors for stage III CRC. A combination of TD and LNR could be used to identify those at high-risk of CRC deaths. [ABSTRACT FROM AUTHOR]
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- 2024
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7. Factors Associated With an Electronic Health Record–Based Definition of Postacute Sequelae of COVID-19 in Patients With Systemic Autoimmune Rheumatic Disease
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Patel, Naomi J., Wang, Xiaosong, Lin, Miao, Kowalski, Emily N., Cook, Claire E., Vanni, Kathleen M.M., Guzzo, Krishan, Qian, Grace, Bade, Katarina J., Saavedra, Alene, Venkat, Rathnam, Srivatsan, Shruthi, Williams, Zachary K., Hanberg, Jennifer S., Kawano, Yumeko, Schiff, Abigail E., Sparks, Jeffrey A., and Wallace, Zachary S.
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ObjectiveMany individuals with rheumatic disease are at higher risk for severe acute coronavirus disease 2019 (COVID-19). We aimed to evaluate risk factors for postacute sequelae of COVID-19 (PASC) using an electronic health record (EHR)-based definition.MethodsWe identified patients with prevalent rheumatic diseases and COVID-19 within the Mass General Brigham healthcare system. PASC was defined by the International Classification of Diseases, 10th revision (ICD-10) codes, relevant labs, vital signs, and medications at least 30 days following the first COVID-19 infection. Patients were followed until the earliest of incident PASC, repeat COVID-19 infection, 1 year of follow-up, death, or February 19, 2023. We used multivariable Cox regression to estimate the association of baseline characteristics with PASC risk.ResultsAmong 2459 patients (76.37% female, mean age 57.4 years), the most common incident PASC manifestations were cough (14.56%), dyspnea (12.36%), constipation (11.39%), and fatigue (10.70%). Serious manifestations including acute coronary disease (4.43%), thromboembolism (3.09%), hypoxemia (3.09%), stroke (1.75%), and myocarditis (0.12%) were rare. The Delta wave (adjusted hazard ratio [aHR] 0.63, 95% CI 0.49-0.82) and Omicron era (aHR 0.50, 95% CI 0.41-0.62) were associated with lower risk of PASC than the early pandemic period (March 2020–June 2021). Age, obesity, comorbidity burden, race, and hospitalization for acute COVID-19 infection were associated with greater risk of PASC. Glucocorticoid (GC) use (aHR 1.19, 95% CI 1.05-1.34 compared to no use) was associated with greater risk of PASC.ConclusionAmong patients with rheumatic diseases, following their first COVID-19 infection, we found a decreased risk of PASC over calendar time using an EHR-based definition. Aside from GCs, no specific immunomodulatory medications were associated with increased risk, and risk factors were otherwise similar to those seen in the general population.
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- 2024
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8. Elevated CDKN1A (P21) mediates β-thalassemia erythroid apoptosis, but its loss does not improve β-thalassemic erythropoiesis
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Liang, Raymond, Lin, Miao, Menon, Vijay, Qiu, Jiajing, Menon, Anagha, Breda, Laura, Arif, Tasleem, Rivella, Stefano, and Ghaffari, Saghi
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•Elevated P21 or FOXO3 mediates β-thalassemia erythroid cell apoptosis.•Loss of FOXO3 or P21 reduces β-thalassemia erythroid cell apoptosis but loss of neither improves β-thalassemic ineffective erythropoiesis.
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- 2023
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9. Controlled Encapsulation of Gold Nanoparticles into Zr-Metal–Organic Frameworks with Improved Detection Limitation of Volatile Organic Compounds via Surface-Enhanced Raman Scattering.
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Lam, Phuc Khanh, Liao, Jian-Jie, Lin, Miao-Chun, Li, Yu-Hsiu, Wang, Tsu-Hao, Huang, Hsin-Kai, Hsu, Yu-An, Hsieh, Hao-Ying Patterson, Kuan, Pu-Yun, Chen, Ching-Tien, Hao, Guo-Xiu, Tsung, Chia-Kuang, Wu, Kevin C.-W., Šutka, Andris, Kinka, Martynas, Chou, Lien-Yang, and Shieh, Fa-Kuen
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- 2023
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10. Suspension Trapping-Based Sample Preparation Workflow for In-Depth Plant Phosphoproteomics.
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Chen, Chin-Wen, Tsai, Chia-Feng, Lin, Miao-Hsia, Lin, Shu-Yu, and Hsu, Chuan-Chih
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- 2023
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11. Analyzing Fundamental Properties of Two-Dimensional Materials by Raman Spectroscopy from Microscale to Nanoscale.
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Wu, Heng, Lin, Miao-Ling, Zhou, Yan, Zhang, Xin, and Tan, Ping-Heng
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- 2023
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12. Abnormal Out-of-Plane Vibrational Raman Mode in Electrochemically Intercalated Multilayer MoS2.
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Sun, Yufei, Yin, Shujia, Peng, Ruixuan, Liang, Jia, Cong, Xin, Li, Yi, Li, Chenyu, Wang, Bolun, Lin, Miao-Ling, Tan, Ping-Heng, Wan, Chunlei, and Liu, Kai
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- 2023
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13. Enhanced Layer-Breathing Modes in van der Waals Heterostructures Based on Twisted Bilayer Graphene.
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Hao, He, Lin, Miao-Ling, Xu, Bo, Wu, Heng, Wang, Yuechen, Peng, Hailin, Tan, Ping-Heng, Tong, Lianming, and Zhang, Jin
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- 2023
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14. Suspension Trapping-Based Sample Preparation Workflow for In-Depth Plant Phosphoproteomics
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Chen, Chin-Wen, Tsai, Chia-Feng, Lin, Miao-Hsia, Lin, Shu-Yu, and Hsu, Chuan-Chih
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Plant phosphoproteomics provides a global view of phosphorylation-mediated signaling in plants; however, it demands high-throughput methods with sensitive detection and accurate quantification. Despite the widespread use of protein precipitation for removing contaminants and improving sample purity, it limits the sensitivity and throughput of plant phosphoproteomic analysis. The multiple handling steps involved in protein precipitation lead to sample loss and process variability. Herein, we developed an approach based on suspension trapping (S-Trap), termed tandem S-Trap-IMAC (immobilized metal ion affinity chromatography), by integrating an S-Trap micro-column with a Fe-IMAC tip. Compared with a precipitation-based workflow, the tandem S-Trap-IMAC method deepened the coverage of the Arabidopsis (Arabidopsis thaliana) phosphoproteome by more than 30%, with improved number of multiply phosphorylated peptides, quantification accuracy, and short sample processing time. We applied the tandem S-Trap-IMAC method for studying abscisic acid (ABA) signaling in Arabidopsis seedlings. We thus discovered that a significant proportion of the phosphopeptides induced by ABA are multiply phosphorylated peptides, indicating their importance in early ABA signaling and quantified several key phosphorylation sites on core ABA signaling components across four time points. Our results show that the optimized workflow aids high-throughput phosphoproteome profiling of low-input plant samples.
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- 2023
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15. Control of Raman Scattering Quantum Interference Pathways in Graphene.
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Chen, Xue, Reichardt, Sven, Lin, Miao-Ling, Leng, Yu-Chen, Lu, Yan, Wu, Heng, Mei, Rui, Wirtz, Ludger, Zhang, Xin, Ferrari, Andrea C., and Tan, Ping-Heng
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- 2023
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16. Analyzing Fundamental Properties of Two-Dimensional Materials by Raman Spectroscopy from Microscale to Nanoscale
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Wu, Heng, Lin, Miao-Ling, Zhou, Yan, Zhang, Xin, and Tan, Ping-Heng
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- 2023
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17. Enhanced Layer-Breathing Modes in van der Waals Heterostructures Based on Twisted Bilayer Graphene
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Hao, He, Lin, Miao-Ling, Xu, Bo, Wu, Heng, Wang, Yuechen, Peng, Hailin, Tan, Ping-Heng, Tong, Lianming, and Zhang, Jin
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The characterization of interlayer coupling in two-dimensional van der Waals heterostructures (vdWHs) is essential to understand their quantum behaviors and structural functionalities. Interlayer shear and layer-breathing (LB) phonons carry rich information on interlayer interaction, but they are usually too weak to be detected via standard Raman spectroscopy due to the weak electron–phonon coupling (EPC). Here, we report a universal strategy to enhance LB modes of vdWHs based on twisted bilayer graphene (tBLG). In both tBLG/hBN and tBLG/MoS2vdWHs, the resonantly excited electrons in tBLG can strongly couple to LB phonons extended over the entire layers in the vdWHs, whose resonance condition is tunable by the twist angle of tBLG. In vdWHs containing twisted graphene layers with multiple twisted interfaces, the EPC of LB phonons coming from the collective LB vibrations of entire heterostructure layers can be tuned by resonant excitation of programmable van Hove singularities according to each twisted interface. The universality and tunability of enhanced LB phonons by tBLG make it a promising method to investigate EPC and interlayer interaction in related vdWHs.
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- 2023
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18. Control of Raman Scattering Quantum Interference Pathways in Graphene
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Chen, Xue, Reichardt, Sven, Lin, Miao-Ling, Leng, Yu-Chen, Lu, Yan, Wu, Heng, Mei, Rui, Wirtz, Ludger, Zhang, Xin, Ferrari, Andrea C., and Tan, Ping-Heng
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Graphene is an ideal platform to study the coherence of quantum interference pathways by tuning doping or laser excitation energy. The latter produces a Raman excitation profile that provides direct insight into the lifetimes of intermediate electronic excitations and, therefore, on quantum interference, which has so far remained elusive. Here, we control the Raman scattering pathways by tuning the laser excitation energy in graphene doped up to 1.05 eV. The Raman excitation profile of the G mode indicates its position and full width at half-maximum are linearly dependent on doping. Doping-enhanced electron–electron interactions dominate the lifetimes of Raman scattering pathways and reduce Raman interference. This will provide guidance for engineering quantum pathways for doped graphene, nanotubes, and topological insulators.
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- 2023
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19. Long-range ordered porous carbons produced from C60
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Pan, Fei, Ni, Kun, Xu, Tao, Chen, Huaican, Wang, Yusong, Gong, Ke, Liu, Cai, Li, Xin, Lin, Miao-Ling, Li, Shengyuan, Wang, Xia, Yan, Wensheng, Yin, Wen, Tan, Ping-Heng, Sun, Litao, Yu, Dapeng, Ruoff, Rodney S., and Zhu, Yanwu
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Carbon structures with covalent bonds connecting C60molecules have been reported1–3, but their production methods typically result in very small amounts of sample, which restrict the detailed characterization and exploration necessary for potential applications. We report the gram-scale preparation of a new type of carbon, long-range ordered porous carbon (LOPC), from C60powder catalysed by α-Li3N at ambient pressure. LOPC consists of connected broken C60cages that maintain long-range periodicity, and has been characterized by X-ray diffraction, Raman spectroscopy, magic-angle spinning solid-state nuclear magnetic resonance spectroscopy, aberration-corrected transmission electron microscopy and neutron scattering. Numerical simulations based on a neural network show that LOPC is a metastable structure produced during the transformation from fullerene-type to graphene-type carbons. At a lower temperature, shorter annealing time or by using less α-Li3N, a well-known polymerized C60crystal forms owing to the electron transfer from α-Li3N to C60. The carbon K-edge near-edge X-ray absorption fine structure shows a higher degree of delocalization of electrons in LOPC than in C60(s). The electrical conductivity is 1.17 × 10−2S cm−1at room temperature, and conduction at T< 30 K appears to result from a combination of metallic-like transport over short distances punctuated by carrier hopping. The preparation of LOPC enables the discovery of other crystalline carbons starting from C60(s).
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- 2023
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20. Immunosuppression impaired the immunogenicity of inactivated SARS-CoV-2 vaccine in non-dialysis kidney disease patients.
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Zhang, Yue-Miao, Liu, Xing-Zi, Lin, Miao-Miao, Zan, Jin-Can, Hu, Yi-Tong, Wang, Xiang-Qiu, Wu, Wen-Qi, Zhou, Tai-Cheng, Lv, Ji-Cheng, Zhang, Hong, Yang, Li, and Zhang, Zi-Jie
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- 2022
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21. Investigation on AEB Key Parameters for Improving Car to Two-Wheeler Collision Safety Using In-Depth Traffic Accident Data
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Hu, Lin, Li, Haibo, Yi, Ping, Huang, Jing, Lin, Miao, and Wang, Hong
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Automatic emergency braking (AEB) system play an essential role in avoiding traffic collisions and minimizing impact strengths, which contributes to improving road traffic safety. However, the present research of the AEB system is mainly concentrated on avoiding the car to car and car to pedestrian collisions. China has typical mixed traffic characteristics, especially the frequent car to two-wheeler collision accidents. Research on the AEB system to prevent car to two-wheeler collisions in complex multi-traffic scenarios is of great significance to further improve the comprehensive performance of the AEB system. In this article, based on the cases of the car to two-wheeler collisions from China In-Depth Accident Study (CIDAS) database, the effects of three key parameters of the AEB system, braking deceleration, braking advance time, and detection range, on the accident rate and severity are studied by accident reconstruction and virtual experiment. Results show that the theoretical optimal detection range of the AEB system is 180°–35 m for the car to two-wheeler collision prevention. Moreover, the current feasible optimal detection range is 120°–35 m due to the limitation of environmental awareness technology. When the braking advance time is 1 s, the detection angle is 120°, and the detection distance is 35 m, the installation of the AEB system can avoid 22.3% of the car to two-wheeler collision accidents. Meanwhile, in the inevitable accidents, the average collision speed of vehicles decreases from 33.93 to 21.34 km/h, which effectively reduces the collision strengths. Findings of this study could help car manufacturers and makers of AEB hardware select the appropriate detection radar. They can also provide ideas and data support for formulating relevant laws and regulations of the AEB system, future development of AEB system, or other driver assistance systems.
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- 2023
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22. Analysis of factors affecting riders injury severity in E-bike-motor vehicle collision accidents based on CIDAS data
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Easa, Said, Wang, Peng, Lin, Miao, Li, XiaoHu, Wang, WenXia, and Wei, Wen
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- 2022
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23. Doxycycline vs Placebo at 12 Weeks in Patients With Mild Thyroid-Associated Ophthalmopathy: A Randomized Clinical Trial
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Pan, Yuan, Chen, Yu-Xi, Zhang, Jian, Lin, Miao-Li, Liu, Guang-Ming, Xu, Xue-Liang, Fan, Xian-Qun, Zhong, Yong, Li, Qing, Ai, Si-Ming, Xu, Wen, Tan, Jia, Zhou, Hui-Fang, Xu, Dong-Dong, Zhang, Hui-Ying, Xu, Bei, Wang, Sha, Ma, Jun-Jie, Zhang, Shuo, Gan, Lin-Yang, Cui, Jian-Tao, Li, Li, Xie, Yan-Yan, Guo, Xinxing, Pan-Doh, Nathan, Zhu, Zhuo-Ting, Lu, Yao, Shi, Yu-Xun, Xia, Yi-Wen, Li, Zuo-Yi, and Liang, Dan
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IMPORTANCE: Mild thyroid-associated ophthalmopathy (TAO) negatively impacts quality of life, yet no clinical guidelines for its treatment are available. Existing evidence supports the use of doxycycline in treating mild TAO. OBJECTIVE: To evaluate the short-term (12 weeks) efficacy of doxycycline in treating mild TAO. DESIGN, SETTING, AND PARTICIPANTS: In this placebo-controlled multicenter randomized double-masked trial, 148 patients were assessed for eligibility. After exclusions (patients who were pregnant or lactating, had an allergy to tetracyclines, or had uncontrolled systematic diseases), 100 patients with mild TAO (orbital soft tissue affected mildly) at 5 centers in China were enrolled from July 2013 to December 2019 and monitored for 12 weeks. INTERVENTIONS: Participants were randomly assigned 1:1 to receive doxycycline (50 mg) or placebo once daily for 12 weeks. MAIN OUTCOMES AND MEASURES: The primary outcome was the rate of improvement at 12 weeks compared with baseline assessed by a composite indicator of eyelid aperture (reduction ≥2 mm), proptosis (reduction ≥2 mm), ocular motility (increase ≥8°), and Graves ophthalmopathy-specific quality-of-life (GO-QOL) scale score (increase ≥6 points). Adverse events were recorded. RESULTS: A total of 50 participants were assigned to doxycycline and 50 to placebo. The mean (SD) age was 36.7 (9.1) years; 75 participants (75.0%) were female and 100 (100.0%) were Asian. Medication compliance was checked during participant interviews and by counting excess tablets. At week 12, the improvement rate was 38.0% (19 of 50) in the doxycycline group and 16.0% (8 of 50) in the placebo group (difference, 22.0%; 95% CI, 5.0-39.0; P = .01) in the intention-to-treat population. The per-protocol sensitivity analysis showed similar results (39.6% [19 of 48] vs 16.0% [8 of 50]; difference, 23.6%; 95% CI, 6.4-40.8; P = .009). No adverse events other than 1 case of mild gastric acid regurgitation was recorded in either group. CONCLUSIONS AND RELEVANCE: The results of this study indicate that oral doxycycline, 50 mg daily, resulted in greater improvement of TAO-related symptoms at 12 weeks compared with placebo in patients with mild TAO. These findings support the consideration of doxycycline for mild TAO but should be tempered by recognizing the relatively short follow-up and the size of the cohort. TRIAL REGISTRATION: ClinicalTrials.gov Identifier: NCT02203682
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- 2022
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24. Superconductivity in Layered van der Waals Hydrogenated Germanene at High Pressure
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Xi, Yilian, Jing, Xiaoling, Xu, Zhongfei, Liu, Nana, Liu, Yani, Lin, Miao-Ling, Yang, Ming, Sun, Ying, Zhuang, Jincheng, Xu, Xun, Hao, Weichang, Li, Yanchun, Li, Xiaodong, Wei, Xiangjun, Tan, Ping-Heng, Li, Quanjun, Liu, Bingbing, Dou, Shi Xue, and Du, Yi
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The emergence of superconductivity in two-dimensional (2D) materials has attracted tremendous research efforts because the origins and mechanisms behind the unexpected and fascinating superconducting phenomena remain unclear. In particular, the superconductivity can survive in 2D systems even with weakened disorder and broken spatial inversion symmetry. Here, structural and superconducting transitions of 2D van der Waals (vdW) hydrogenated germanene (GeH) are observed under compression and decompression processes. GeH possesses a superconducting transition with a critical temperature (Tc) of 5.41 K at 8.39 GPa. A crystalline to amorphous transition occurs at 16.80 GPa, while superconductivity remains. An abnormal increase of Tcup to 6.11 K was observed during the decompression process, while the GeH remained in the 2D amorphous phase. A combination study of in situhigh-pressure synchrotron X-ray diffraction, in situhigh-pressure Raman spectroscopy, transition electron microscopy, and density functional theory simulations suggests that the superconductivity in 2D vdW GeH is attributed to the increased density of states at the Fermi level as well as the enhanced electron–phonon coupling effect under high pressure even in the form of an amorphous phase. The unique pressure-induced phase transition of GeH from 2D crystalline to 2D amorphous metal hydride provides a promising platform to study the mechanisms of amorphous hydride superconductivity.
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- 2022
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25. Mitochondrial-derived damage-associated molecular patterns amplify neuroinflammation in neurodegenerative diseases
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Lin, Miao-miao, Liu, Na, Qin, Zheng-hong, and Wang, Yan
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Both mitochondrial dysfunction and neuroinflammation are implicated in neurodegeneration and neurodegenerative diseases. Accumulating evidence shows multiple links between mitochondrial dysfunction and neuroinflammation. Mitochondrial-derived damage-associated molecular patterns (DAMPs) are recognized by immune receptors of microglia and aggravate neuroinflammation. On the other hand, inflammatory factors released by activated glial cells trigger an intracellular cascade, which regulates mitochondrial metabolism and function. The crosstalk between mitochondrial dysfunction and neuroinflammatory activation is a complex and dynamic process. There is strong evidence that mitochondrial dysfunction precedes neuroinflammation during the progression of diseases. Thus, an in-depth understanding of the specific molecular mechanisms associated with mitochondrial dysfunction and the progression of neuroinflammation in neurodegenerative diseases may contribute to the identification of new targets for the treatment of diseases. In this review, we describe in detail the DAMPs that induce or aggravate neuroinflammation in neurodegenerative diseases including mtDNA, mitochondrial unfolded protein response (mtUPR), mitochondrial reactive oxygen species (mtROS), adenosine triphosphate (ATP), transcription factor A mitochondria (TFAM), cardiolipin, cytochrome c, mitochondrial Ca2+and iron.
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- 2022
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26. Macrophage-intrinsic DUOX1 contributes to type 2 inflammation and mucus metaplasia during allergic airway disease
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Morris, Carolyn R., Habibovic, Aida, Dustin, Christopher M., Schiffers, Caspar, Lin, Miao-Chong, Ather, Jennifer L., Janssen-Heininger, Yvonne M.W., Poynter, Matthew E., Utermohlen, Olaf, Krönke, Martin, and van der Vliet, Albert
- Abstract
The NADPH oxidase DUOX1 contributes to epithelial production of alarmins, including interleukin (IL)-33, in response to injurious triggers such as airborne protease allergens, and mediates development of mucus metaplasia and airway remodeling in chronic allergic airways diseases. DUOX1 is also expressed in non-epithelial lung cell types, including macrophages that play an important role in airway remodeling during chronic lung disease. We therefore conditionally deleted DUOX1 in either lung epithelial or monocyte/macrophage lineages to address its cell-specific actions in innate airway responses to acute airway challenge with house dust mite (HDM) allergen, and in chronic HDM-driven allergic airway inflammation. As expected, acute responses to airway challenge with HDM, as well as type 2 inflammation and related features of airway remodeling during chronic HDM-induced allergic inflammation, were largely driven by DUOX1 with the respiratory epithelium. However, in the context of chronic HDM-driven inflammation, DUOX1 deletion in macrophages also significantly impaired type 2 cytokine production and indices of mucus metaplasia. Further studies revealed a contribution of macrophage-intrinsic DUOX1 in macrophage recruitment upon chronic HDM challenge, as well as features of macrophage activation that impact on type 2 inflammation and remodeling.
- Published
- 2022
- Full Text
- View/download PDF
27. Photoelectron Spectroscopy and Density Functional Investigation of the Structural Evolution, Electronic, and Magnetic Properties of CrSin– (n = 14–18) Clusters.
- Author
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Kai Wang, Hong-Yuan Zhao, Lin Miao, Ze-Zhao Jia, Guang-Jia Yin, Xiao-Dong Zhu, Moro, Ramiro, von Issendorff, Bernd, and Lei Ma
- Published
- 2022
- Full Text
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28. Divergent On-DNA Transformations from DNA-Linked Piperidones.
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Xing Ling, Weiwei Lu, Lin Miao, Marcaurelle, Lisa A., Xuan Wang, Yun Ding, and Xiaojie Lu
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- 2022
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29. Lipid-Based Nanocarrier Systems for Drug Delivery: Advances and Applications
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Zhao, Yan-Qi, Li, Li-Jun, Zhou, Er-Fen, Wang, Jiang-Yue, Wang, Ying, Guo, Lin-Miao, and Zhang, Xin-Xin
- Published
- 2022
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30. Clinicopathological characteristics, risk factors and renal outcome in IgA nephropathy with crescents
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Ruan, Yiping, Hong, Fuyuan, Wu, Jiabin, Lin, Miao, Wang, Chen, Lian, Fayang, Cao, Fang, Yang, Guokai, Huang, Lanting, and Huang, Qiaoyun
- Abstract
Background/aims: The aim of the study was to investigate the clinicopathological characteristics, risk factors and renal outcome in IgA nephropathy (IgAN) patients with crescents. Methods: Four hundred and fifty-eight biopsy-proven primary IgAN patients included between January 2010 and October 2021 for a retrospective analysis were divided into three groups according to crescent score of the updated Oxford classification: C0 group (n = 255), C1 group (n = 187) and C2 group (n = 16). The clinicopathological features and renal outcomes were recorded. In univariate and multivariate models, the association between crescents and renal outcome and C2-associated clinical factors were analyzed. Results: Patients with a higher proportion of crescents presented worse clinical characteristics with regard to kidney function, proteinuria, hematuria, hemoglobin, uric acid, cholesterol, and serum albumin, while global glomerulosclerosis, segmental adhesion, tuft necrosis, segmental glomerulosclerosis (S1), tubular atrophy/interstitial fibrosis (T1/2), and lymphocyte and monocyte infiltration were more severe. By multivariate logistic regression analysis, eGFR (OR 0.981, 95% CI 0.964–0.999, P = 0.039), proteinuria (OR 1.655, 95% CI 1.180–2.321, P = 0.004), and hematuria (OR 4.752, 95% CI 1.426–15.835, P = 0.011) were significantly associated with C2. C2 was significantly associated with poorer renal survival even in patients receiving immunosuppressive therapy. Nevertheless, only eGFR at baseline, rather than crescents, was an independent predictor for renal survival in multivariate Cox analyses. Conclusion: IgAN patients with crescents presented more severe clinical and pathological features. Renal function, proteinuria and hematuria contributed to identifying patients with crescents. Crescents were associated with poorer renal survival, even in patients receiving immunosuppressive therapy, but it was not an independent predictor. Graphical abstract:
- Published
- 2022
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31. FHL2 activates β-catenin/Wnt signaling by complexing with APC and TRIM63 in lung adenocarcinoma
- Author
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Gao, Jian, Ao, Yong-Qiang, Deng, Jie, Lin, Miao, Wang, Shuai, Jiang, Jia-Hao, and Ding, Jian-Yong
- Abstract
•Highly expressed FHL2 is correlated with poor prognosis in LUAD patients.•FHL2 facilitates the progression of LUAD in vitro and in vivo.•FHL2 acts as a scaffold for APC and TRIM63 in LUAD.•FHL2 enhances TRIM63 mediated ubiquitination of APC and stabilizes β-catenin/Wnt signaling pathway.
- Published
- 2024
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32. Investigation of ferroptosis-associated molecular subtypes and immunological characteristics in lupus nephritis based on artificial neural network learning
- Author
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Zhang, Li, Yan, Qing, Lin, Miao, He, Juanjuan, Tian, Jie, Chen, Zhihan, and Hong, Fuyuan
- Abstract
Background: Lupus nephritis (LN) is a severe complication of systemic lupus erythematosus (SLE) with poor treatment outcomes. The role and underlying mechanisms of ferroptosis in LN remain largely unknown. We aimed to explore ferroptosis-related molecular subtypes and assess their prognostic value in LN patients. Methods: Molecular subtypes were classified on the basis of differentially expressed ferroptosis-related genes (FRGs) via the Consensus ClusterPlus package. The enriched functions and pathways, immune infiltrating levels, immune scores, and immune checkpoints were compared between the subgroups. A scoring algorithm based on the subtype-specific feature genes identified by artificial neural network machine learning, referred to as the NeuraLN, was established, and its immunological features, clinical value, and predictive value were evaluated in patients with LN. Finally, immunohistochemical analysis was performed to validate the expression and role of feature genes in glomerular tissues from LN patients and controls. Results: A total of 10 differentially expressed FRGs were identified, most of which showed significant correlation. Based on the 10 FRGs, LN patients were classified into two ferroptosis subtypes, which exhibited significant differences in immune cell abundances, immune scores, and immune checkpoint expression. A NeuraLN-related protective model was established based on nine subtype-specific genes, and it exhibited a robustly predictive value in LN. The nomogram and calibration curves demonstrated the clinical benefits of the protective model. The high-NeuraLN group was closely associated with immune activation. Clinical specimens demonstrated the alterations of ALB, BHMT, GAMT, GSTA1, and HAO2 were in accordance with bioinformatics analysis results, GSTA1 and BHMT were negatively correlated with the severity of LN. Conclusion: The classification of ferroptosis subtypes and the establishment of a protective model may form a foundation for the personalized treatment of LN patients.
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- 2024
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33. Bathymetric effect on geoid modeling over the Great Lakes area
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Li, Xiaopeng, Lin, Miao, Krcmaric, Jordan, and Carignan, Kelly
- Abstract
Graphical Abstract:
- Published
- 2024
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34. Long distance high resolution FMCW laser ranging with phase noise compensation and 2D signal processing
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Ke, Jia-Yi, Song, Zi-Qi, Wang, Pei-Si, Cui, Zhong-Ming, Mo, Di, Lin, Miao, Wang, Ran, and Wu, Jin
- Abstract
A long distance high resolution frequency-modulated continuous wave (FMCW) laser rangefinder with phase noise compensation and two-dimensional (2D) data processing skills is developed. Range-finding ladar consists of a continuously chirped laser source, an auxiliary reference interferometer, and a monostatic optical transceiver for target illumination and return photon collection. To extend the range unambiguity and lower the electronic processing bandwidth, a two-step laser frequency chirping scheme is adopted, where a long pulse width, small frequency bandwidth laser chirping signal are used in step 1 for coarse distance estimation, and a short pulse width and large frequency bandwidth laser chirping signal are applied afterwards for step 2 high resolution distance realization. An auxiliary reference interferometer is to record the phase noise originated from the laser source to compensate for phase errors induced in the target return photons. The 2D data processing skill helps to coherently sum up all the phase noise removed echo photons to achieve high resolution range peak extraction with high detection sensitivity. Experimental demonstration shows that the proposed FMCW ladar at 1550 nm wavelength with a laser chirping bandwidth of 10 GHz and electronic processing bandwidth of 200 MHz can measure a corner cube test target in an outdoor atmospheric environment, and the measurement results are 12013.905 m with a 2.4 cm range resolution under strong return photon levels and 12013.920 m with a 2.5 cm range resolution under weak return photon levels.
- Published
- 2022
35. Dietary inflammatory index and cardiorenal function in women with diabetes and prediabetes.
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Lin, Miao, Shivappa, Nitin, Hébert, James R., Huang, Huibin, Cai, Liangchun, Liang, Jixing, Lin, Wei, Zong, Liyao, Wang, Nengying, Li, Liantao, Lin, Lixiang, Lu, Jieli, Bi, Yufang, Wang, Weiqing, Wen, Junping, and Chen, Gang
- Abstract
Background and Aims: Previous studies had shed a new light on the importance of multiple inflammatory mechanisms in the pathogenesis of arterial stiffness. The dietary inflammatory index (DII®) is a new tool for estimating the overall inflammatory potential of the diet. The aim of this study is to assess the association of the inflammatory potential of diet with peripheral arterial stiffness and renal function in women with diabetes and prediabetes.Methods and Results: This is an observational cross-sectional study. A total of 2644 females aged 45-75 years were included for the study. Dietary intake in the past 12 months was assessed by a validated China National Nutrition and Health Survey 2002 (CNHS2002) food-frequency questionnaire. Energy-adjusted DII (E-DII) scores were calculated from daily dietary information. In a multivariable linear regression analysis adjusted for potential confounders, E-DII was positively associated with brachial ankle pulse wave velocity (baPWV) in participants with diabetes (β = 12.820; 95% CI = 2.565, 23.076; P = 0.014) and prediabetes (β = 29.025; 95% CI = 1.110, 56.940; P = 0.042), but not in females with normal glucose homeostasis. In addition, per unit increase of E-DII was significantly associated with lower eGFR (β = -1.363; 95% CI = -2.335, -0.392; P = 0.006) in patients with diabetes.Conclusion: We identified a direct association between E-DII and arterial stiffness, decreasedeGFR in middle-aged and elderly women with diabetes or prediabetes. Future studies are needed to verify and clarify the role of E-DII as an intervention target for cardiorenal complications of chronic hyperglycemia. [ABSTRACT FROM AUTHOR]- Published
- 2021
- Full Text
- View/download PDF
36. Size-Resolved Single Entity Collision Biosensing for Dual Quantification of MicroRNAs in a Single Run.
- Author
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Bai, Yi-Yan, Yang, Yan-Ju, Wu, Zhen, Yang, Xiao-Yan, Lin, Miao, Pang, Dai-Wen, and Zhang, Zhi-Ling
- Published
- 2021
- Full Text
- View/download PDF
37. ALK fusion promotes metabolic reprogramming of cancer cells by transcriptionally upregulating PFKFB3
- Author
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Hu, Mengnan, Bao, Ruoxuan, Lin, Miao, Han, Xiao-Ran, Ai, Ying-Jie, Gao, Yun, Guan, Kun-Liang, Xiong, Yue, and Yuan, Hai-Xin
- Abstract
Anaplastic lymphoma kinase (ALK), a receptor tyrosine kinase of the insulin receptor kinase subfamily, is activated in multiple cancer types through translocation or overexpression. Although several generations of ALK tyrosine kinase inhibitors (TKIs) have been developed for clinic use, drug resistance remains a major challenge. In this study, by quantitative proteomic approach, we identified the glycolytic regulatory enzyme, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), as a new target of ALK. Expression of PFKFB3 is highly dependent on ALK activity in ALK+anaplastic large cell lymphoma and non-small-cell lung cancer (NSCLC) cells. Notably, ALK and PFKFB3 expressions exhibit significant correlation in clinic ALK+NSCLC samples. We further demonstrated that ALK promotes PFKFB3 transcription through the downstream transcription factor STAT3. Upregulation of PFKFB3 by ALK is important for high glycolysis level as well as oncogenic activity of ALK+lymphoma cells. Finally, targeting PFKFB3 by its inhibitor can overcome drug resistance in cells bearing TKI-resistant mutants of ALK. Collectively, our studies reveal a novel ALK–STAT3–PFKFB3 axis to promote cell proliferation and tumorigenesis, providing an alternative strategy for the treatment of ALK-positive tumors.
- Published
- 2022
- Full Text
- View/download PDF
38. Macrophage-intrinsic DUOX1 contributes to type 2 inflammation and mucus metaplasia during allergic airway disease
- Author
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Morris, Carolyn R., Habibovic, Aida, Dustin, Christopher M., Schiffers, Caspar, Lin, Miao-Chong, Ather, Jennifer L., Janssen-Heininger, Yvonne M. W., Poynter, Matthew E., Utermohlen, Olaf, Krönke, Martin, and van der Vliet, Albert
- Abstract
The NADPH oxidase DUOX1 contributes to epithelial production of alarmins, including interleukin (IL)-33, in response to injurious triggers such as airborne protease allergens, and mediates development of mucus metaplasia and airway remodeling in chronic allergic airways diseases. DUOX1 is also expressed in non-epithelial lung cell types, including macrophages that play an important role in airway remodeling during chronic lung disease. We therefore conditionally deleted DUOX1 in either lung epithelial or monocyte/macrophage lineages to address its cell-specific actions in innate airway responses to acute airway challenge with house dust mite (HDM) allergen, and in chronic HDM-driven allergic airway inflammation. As expected, acute responses to airway challenge with HDM, as well as type 2 inflammation and related features of airway remodeling during chronic HDM-induced allergic inflammation, were largely driven by DUOX1 with the respiratory epithelium. However, in the context of chronic HDM-driven inflammation, DUOX1 deletion in macrophages also significantly impaired type 2 cytokine production and indices of mucus metaplasia. Further studies revealed a contribution of macrophage-intrinsic DUOX1 in macrophage recruitment upon chronic HDM challenge, as well as features of macrophage activation that impact on type 2 inflammation and remodeling.
- Published
- 2022
- Full Text
- View/download PDF
39. Integrative Analysis of Genome, 3D Genome, and Transcriptome Alterations of Clinical Lung Cancer Samples
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Li, Tingting, Li, Ruifeng, Dong, Xuan, Shi, Lin, Lin, Miao, Peng, Ting, Wu, Pengze, Liu, Yuting, Li, Xiaoting, He, Xuheng, Han, Xu, Kang, Bin, Wang, Yinan, Liu, Zhiheng, Chen, Qing, Shen, Yue, Feng, Mingxiang, Wang, Xiangdong, Wu, Duojiao, Wang, Jian, and Li, Cheng
- Abstract
Genomic studies of cancer cell alterations, such as mutations, copy number variations(CNVs), and translocations, greatly promote our understanding of the genesis and development of cancers. However, the 3D genomearchitecture of cancers remains less studied due to the complexity of cancer genomes and technical difficulties. To explore the 3D genome structure in clinical lung cancer, we performed Hi-C experiments using paired normal and tumor cells harvested from patients with lung cancer, combining with RNA sequenceing analysis. We demonstrated the feasibility of studying 3D genome of clinical lung cancer samples with a small number of cells (1 × 104), compared the genome architecture between clinical samplesand cell lines of lung cancer, and identified conserved and changed spatial chromatin structures between normal and cancer samples. We also showed that Hi-C data can be used to infer CNVs and point mutations in cancer. By integrating those different types of cancer alterations, we showed significant associations between CNVs, 3D genome, and gene expression. We propose that 3D genome mediates the effects of cancer genomic alterations on gene expression through altering regulatory chromatin structures. Our study highlights the importance of analyzing 3D genomes of clinical cancer samples in addition to cancer cell lines and provides an integrative genomic analysispipeline for future larger-scale studies in lung cancer and other cancers.
- Published
- 2021
- Full Text
- View/download PDF
40. Chitosan oligosaccharide modified liposomes enhance lung cancer delivery of paclitaxel
- Author
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Miao, Yun-qiu, Chen, Ming-shu, Zhou, Xin, Guo, Lin-miao, Zhu, Jing-jing, Wang, Rui, Zhang, Xin-xin, and Gan, Yong
- Abstract
Lung cancer is one of the leading causes of cancer-related death worldwide. Various therapeutic failed in the effective treatment of the lung cancer due to their limited accumulation and exposure in tumors. In order to promote the chemotherapeutics delivery to lung tumor, we introduced chitosan oligosaccharide (CSO) modification on the liposomes. CSO conjugated Pluronic P123 polymers with different CSO grafting amounts, called as CP50 and CP20, were synthesized and used to prepare CSO modified liposomes (CP50-LSs and CP20-LSs). CP50-LSs and CP20-LSs displayed significantly enhanced cellular uptake in A549 cells in vitro as well as superior tumor accumulation in vivo compared with non-CSO modified liposomes (P-LSs). This phenomenon was related to the increased affinity between CSO modified liposomes and tumor cells following massive adsorption of collagen, which was highly expressed in lung tumors. In the A549 tumor-bearing mouse model, intravenous injection of paclitaxel (PTX)-loaded CP50-LSs every 3 days for 21 days resulted in optimal antitumor therapeutic performance with an inhibition rate of 86.4%. These results reveal that CSO modification provides promising applicability for nanomedicine design in the lung cancer treatment.
- Published
- 2021
- Full Text
- View/download PDF
41. Integrative Analysis of Genome, 3D Genome, and Transcriptome Alterations of Clinical Lung Cancer Samples
- Author
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Li, Tingting, Li, Ruifeng, Dong, Xuan, Shi, Lin, Lin, Miao, Peng, Ting, Wu, Pengze, Liu, Yuting, Li, Xiaoting, He, Xuheng, Han, Xu, Kang, Bin, Wang, Yinan, Liu, Zhiheng, Chen, Qing, Shen, Yue, Feng, Mingxiang, Wang, Xiangdong, Wu, Duojiao, Wang, Jian, and Li, Cheng
- Abstract
Genomic studies of cancer cell alterations, such as mutations, copy number variations(CNVs), and translocations, greatly promote our understanding of the genesis and development of cancers. However, the 3D genomearchitecture of cancers remains less studied due to the complexity of cancer genomes and technical difficulties. To explore the 3D genome structure in clinical lung cancer, we performed Hi-C experiments using paired normal and tumor cells harvested from patients with lung cancer, combining with RNA sequenceing analysis. We demonstrated the feasibility of studying 3D genome of clinical lung cancer samples with a small number of cells (1 × 104), compared the genome architecture between clinical samplesand cell lines of lung cancer, and identified conserved and changed spatial chromatin structures between normal and cancer samples. We also showed that Hi-C data can be used to infer CNVs and point mutations in cancer. By integrating those different types of cancer alterations, we showed significant associations between CNVs, 3D genome, and gene expression. We propose that 3D genome mediates the effects of cancer genomic alterations on gene expression through altering regulatory chromatin structures. Our study highlights the importance of analyzing 3D genomes of clinical cancer samples in addition to cancer cell lines and provides an integrative genomic analysispipeline for future larger-scale studies in lung cancer and other cancers.
- Published
- 2021
- Full Text
- View/download PDF
42. FMCW lidar multitarget detection based on skeleton tree waveform matching
- Author
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Li, Bingchen, Mo, Di, Wang, Peisi, Gan, Nan, Lin, Miao, Wang, Ran, and Li, Shiqiang
- Abstract
Frequency-modulated continuous-wave lidar realizes 4D (three-dimensional space and velocity) imaging of the scene by emitting positive and negative frequency sweep laser signals. The premise of it is to identify the frequency points corresponding to the same target in the positive and negative sweep echo signals. For dechirp receiving, there is usually one peak in the frequency spectrum of the positive and negative sweep signals, respectively. Therefore, it is easy to identify and match the peaks. But in a complex environment, the laser beam will irradiate multiple targets at the same time. In addition, beam scanning and target motion cause the echo spectrum to broaden. The above reasons make it extremely difficult to identify and match peaks in practice. To solve this problem, the waveform-matching algorithm based on the skeleton tree is first applied to multitarget echo pairing. The basic idea of the algorithm is to quantify the target echo hierarchically to generate a skeleton tree. The generation of nodes is based on the relative amplitude of waveform peaks and reflects the characteristics of wave crests nesting. Then the similarity of the signal is determined by comparing the distance between the two signal waveform feature trees. Finally, the waveforms are matched in terms of similarity. To further substantiate the role of the proposed algorithm, imaging experiments and related comparative data for different targets have been completed. The results show that the accuracy of matching processed by the algorithm exceeds 90%, which is improved by about 50% compared with not using the algorithm for the target whose overlapping part accounts for a large proportion of itself.
- Published
- 2021
43. Evaluation Model and Enhancement Strategies for Teaching Reform Capacity of Art Courses in Higher Vocational Colleges.
- Author
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Lin Miao
- Subjects
REFORMS ,COLLEGE teaching ,TEACHING models ,MODEL theory ,UNIVERSITIES & colleges - Abstract
In higher vocational colleges, the teaching reform of art courses faces complex problems, namely, the evaluation system is imperfect, and the evaluation model involves complex calculations. To solve these problems, this paper strives to enhance the teaching reform capacity of art courses in higher vocational colleges. Firstly, the factors affecting the said teaching reform were identified. Next, an index system was established to evaluate the teaching reform capacity. The index system was integrated with the gray theory into an evaluation model of the teaching reform capacity. Based on the evaluation results, several strategies were put forward to enhance the teaching reform capacity. The research results provide a good reference for higher vocational colleges to reform the teaching of art courses. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
44. Mitochondrial localization and moderated activity are key to murine erythroid enucleation
- Author
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Liang, Raymond, Menon, Vijay, Qiu, Jiajing, Arif, Tasleem, Renuse, Santosh, Lin, Miao, Nowak, Roberta, Hartmann, Boris, Tzavaras, Nikos, Benson, Deanna L., Chipuk, Jerry E., Fribourg, Miguel, Pandey, Akhilesh, Fowler, Velia, and Ghaffari, Saghi
- Abstract
Mammalian red blood cells (RBCs), which primarily contain hemoglobin, exemplify an elaborate maturation process, with the terminal steps of RBC generation involving extensive cellular remodeling. This encompasses alterations of cellular content through distinct stages of erythroblast maturation that result in the expulsion of the nucleus (enucleation) followed by the loss of mitochondria and all other organelles and a transition to anaerobic glycolysis. Whether there is any link between erythroid removal of the nucleus and the function of any other organelle, including mitochondria, remains unknown. Here we demonstrate that mitochondria are key to nuclear clearance. Using live and confocal microscopy and high-throughput single-cell imaging, we show that before nuclear polarization, mitochondria progressively move toward one side of maturing erythroblasts and aggregate near the nucleus as it extrudes from the cell, a prerequisite for enucleation to proceed. Although we found active mitochondrial respiration is required for nuclear expulsion, levels of mitochondrial activity identify distinct functional subpopulations, because terminally maturing erythroblasts with low relative to high mitochondrial membrane potential are at a later stage of maturation, contain greatly condensed nuclei with reduced open chromatin–associated acetylation histone marks, and exhibit higher enucleation rates. Lastly, to our surprise, we found that late-stage erythroblasts sustain mitochondrial metabolism and subsequent enucleation, primarily through pyruvate but independent of in situ glycolysis. These findings demonstrate the critical but unanticipated functions of mitochondria during the erythroblast enucleation process. They are also relevant to the in vitro production of RBCs as well as to disorders of the erythroid lineage.
- Published
- 2021
- Full Text
- View/download PDF
45. Mitochondrial localization and moderated activity are key to murine erythroid enucleation
- Author
-
Liang, Raymond, Menon, Vijay, Qiu, Jiajing, Arif, Tasleem, Renuse, Santosh, Lin, Miao, Nowak, Roberta, Hartmann, Boris, Tzavaras, Nikos, Benson, Deanna L., Chipuk, Jerry E., Fribourg, Miguel, Pandey, Akhilesh, Fowler, Velia, and Ghaffari, Saghi
- Abstract
Mammalian red blood cells (RBCs), which primarily contain hemoglobin, exemplify an elaborate maturation process, with the terminal steps of RBC generation involving extensive cellular remodeling. This encompasses alterations of cellular content through distinct stages of erythroblast maturation that result in the expulsion of the nucleus (enucleation) followed by the loss of mitochondria and all other organelles and a transition to anaerobic glycolysis. Whether there is any link between erythroid removal of the nucleus and the function of any other organelle, including mitochondria, remains unknown. Here we demonstrate that mitochondria are key to nuclear clearance. Using live and confocal microscopy and high-throughput single-cell imaging, we show that before nuclear polarization, mitochondria progressively move toward one side of maturing erythroblasts and aggregate near the nucleus as it extrudes from the cell, a prerequisite for enucleation to proceed. Although we found active mitochondrial respiration is required for nuclear expulsion, levels of mitochondrial activity identify distinct functional subpopulations, because terminally maturing erythroblasts with low relative to high mitochondrial membrane potential are at a later stage of maturation, contain greatly condensed nuclei with reduced open chromatin–associated acetylation histone marks, and exhibit higher enucleation rates. Lastly, to our surprise, we found that late-stage erythroblasts sustain mitochondrial metabolism and subsequent enucleation, primarily through pyruvate but independent of in situ glycolysis. These findings demonstrate the critical but unanticipated functions of mitochondria during the erythroblast enucleation process. They are also relevant to the in vitro production of RBCs as well as to disorders of the erythroid lineage.
- Published
- 2021
- Full Text
- View/download PDF
46. Size-Resolved Single Entity Collision Biosensing for Dual Quantification of MicroRNAs in a Single Run
- Author
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Bai, Yi-Yan, Yang, Yan-Ju, Wu, Zhen, Yang, Xiao-Yan, Lin, Miao, Pang, Dai-Wen, and Zhang, Zhi-Ling
- Abstract
Limited to the accuracy of size resolution, single entity collision biosensing (SECBS) for multiplex immunoassays remains challenging, because it is difficult to get the true value of nanoparticle (NP) sizes based on the current intensity due to the complex movement of NPs on the electrode surface. Considering that the size-dependent movement of NPs meanwhile will generate a characteristic current shape, in this work, the huge difference in the current rise time of 5 and 15 nm Pt NPs colliding on an Au ultramicroelectrode (d= 30 μm) was originally used to develop a size-resolved SECBS for multiplex immunoassays of miRNAs. The limit concentration that can be detected was 0.5 fM. Compared with conventional electrochemical biosensors for multiplex immunoassays, for the size-resolved SECBS, one does not need to worry about potential overlapping. Therefore, the proposed method demonstrates a promising potential for the application of SECBS in multiplex immunoassays.
- Published
- 2021
- Full Text
- View/download PDF
47. Functional nano-vector boost anti-atherosclerosis efficacy of berberine in Apoe(−/−) mice.
- Author
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Ma, Xiaolei, Zhang, Tingting, Luo, Zhigang, Li, Xiaolin, Lin, Miao, Li, Rui, Du, Peng, Yu, Xiaoyou, Ma, Chen, Yan, Pengju, Su, Jin, Wang, Lulu, Li, Yuhuan, and Jiang, Jiandong
- Subjects
ADIPOSE tissue diseases ,PERIPHERAL vascular diseases ,HIGH-fat diet ,THORACIC aorta ,MACROPHAGE activation ,BERBERINE - Abstract
Atherosclerosis (AS) is the leading cause of heart attacks, stroke, and peripheral vascular disease. Berberine (BBR), a botanical medicine, has diversified anti-atherosclerotic effects but with poor absorption. The aim of this study was to develop an effective BBR-entrapped nano-system for treating AS in high-fat diet (HFD)-fed Apoe
(−/−) mice, and also explore the possible underlying mechanisms involved. Three d - α -tocopherol polyethylene glycol (PEG) succinate (TPGS) analogues with different PEG chain lengths were synthesized to formulate BBR-entrapped micelles. HFD-fed Apoe(−/−) mice were administered with optimized formula (BBR, 100 mg/kg/day) orally for 5 months. The artery plaque onset and related metabolic disorders were evaluated, and the underlying mechanisms were studied. Our data showed that, BT 1500 M increased BBR deposition in liver and adipose by 107.6% and 172.3%, respectively. In the Apoe(−/−) mice, BT 1500 M ameliorated HFD-induced hyperlipidemia and lipid accumulation in liver and adipose. BT 1500 M also suppressed HFD-induced chronic inflammation as evidenced by the reduced liver and adipose levels of interleukin-6 (IL-6), tumor necrosis factor- α (TNF- α) and interleukin-1 β (IL-1 β); and decreased plasma level of TNF- α , IL-6, IL-1 β , interferon- γ (IFN- γ), monocyte chemotactic protein (MCP), and macrophage inflammatory factor (MIP). The mechanism study showed that BT 1500 M changed Ampk and Nf-κb gene expression, and interrupted a crosstalk process between adipocytes and macrophages. Further investigation proved that BT 1500 M decreased endothelial lesion and subsequent macrophage activation, cytokines release, as well as cholesteryl ester gathering in the aortic arch, resulting in ameliorated artery plaque build-up. Our results provide a practical strategy for treating AS using a BBR-entrapped nano-system. BT 1500 M, which increased gut-absorption and intra-cellular uptake of berberine, modulated AMPK and NF- κ B expression, and improved dyslipidemia and inflammation induced by high fat diet. The endothelial injury and subsequent macrophage infiltration and cholesteryl ester gathering in the aortic arch were decreased, resulting in the inhibition of artery plaque build-up. Image 1 [ABSTRACT FROM AUTHOR]- Published
- 2020
- Full Text
- View/download PDF
48. Using mitochondrial activity to select for potent human hematopoietic stem cells
- Author
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Qiu, Jiajing, Gjini, Jana, Arif, Tasleem, Moore, Kateri, Lin, Miao, and Ghaffari, Saghi
- Abstract
Hematopoietic cell transplantation is a critical curative approach for many blood disorders. However, obtaining grafts with sufficient numbers of hematopoietic stem cells (HSCs) that maintain long-term engraftment remains challenging; this is due partly to metabolic modulations that restrict the potency of HSCs outside of their native environment. To address this, we focused on mitochondria. We found that human HSCs are heterogeneous in their mitochondrial activity as measured by mitochondrial membrane potential (MMP) even within the highly purified CD34+CD38−CD45RA−CD90+CD49f+HSC population. We further found that the most potent HSCs exhibit the lowest mitochondrial activity in the population. We showed that the frequency of long-term culture initiating cells in MMP-low is significantly greater than in MMP-high CD34+CD38−CD45RA−CD90+(CD90+) HSCs. Notably, these 2 populations were distinct in their long-term repopulating capacity when transplanted into immunodeficient mice. The level of chimerism 7 months posttransplantation was >50-fold higher in the blood of MMP-low relative to MMP-high CD90+HSC recipients. Although more than 90% of both HSC subsets were in G0, MMP-low CD90+HSCs exhibited delayed cell-cycle priming profile relative to MMP-high HSCs. These functional differences were associated with distinct mitochondrial morphology; MMP-low in contrast to MMP-high HSCs contained fragmented mitochondria. Our findings suggest that the lowest MMP level selects for the most potent, likely dormant, stem cells within the highly purified HSC population. These results identify a new approach for isolating highly potent human HSCs for further clinical applications. They also implicate mitochondria in the intrinsic regulation of human HSC quiescence and potency.
- Published
- 2021
- Full Text
- View/download PDF
49. Using mitochondrial activity to select for potent human hematopoietic stem cells
- Author
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Qiu, Jiajing, Gjini, Jana, Arif, Tasleem, Moore, Kateri, Lin, Miao, and Ghaffari, Saghi
- Abstract
Hematopoietic cell transplantation is a critical curative approach for many blood disorders. However, obtaining grafts with sufficient numbers of hematopoietic stem cells (HSCs) that maintain long-term engraftment remains challenging; this is due partly to metabolic modulations that restrict the potency of HSCs outside of their native environment. To address this, we focused on mitochondria. We found that human HSCs are heterogeneous in their mitochondrial activity as measured by mitochondrial membrane potential (MMP) even within the highly purified CD34+CD38−CD45RA−CD90+CD49f+ HSC population. We further found that the most potent HSCs exhibit the lowest mitochondrial activity in the population. We showed that the frequency of long-term culture initiating cells in MMP-low is significantly greater than in MMP-high CD34+CD38−CD45RA−CD90+ (CD90+) HSCs. Notably, these 2 populations were distinct in their long-term repopulating capacity when transplanted into immunodeficient mice. The level of chimerism 7 months posttransplantation was >50-fold higher in the blood of MMP-low relative to MMP-high CD90+ HSC recipients. Although more than 90% of both HSC subsets were in G0, MMP-low CD90+ HSCs exhibited delayed cell-cycle priming profile relative to MMP-high HSCs. These functional differences were associated with distinct mitochondrial morphology; MMP-low in contrast to MMP-high HSCs contained fragmented mitochondria. Our findings suggest that the lowest MMP level selects for the most potent, likely dormant, stem cells within the highly purified HSC population. These results identify a new approach for isolating highly potent human HSCs for further clinical applications. They also implicate mitochondria in the intrinsic regulation of human HSC quiescence and potency.
- Published
- 2021
- Full Text
- View/download PDF
50. Phonon renormalization in reconstructed MoS2moiré superlattices
- Author
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Quan, Jiamin, Linhart, Lukas, Lin, Miao-Ling, Lee, Daehun, Zhu, Jihang, Wang, Chun-Yuan, Hsu, Wei-Ting, Choi, Junho, Embley, Jacob, Young, Carter, Taniguchi, Takashi, Watanabe, Kenji, Shih, Chih-Kang, Lai, Keji, MacDonald, Allan H., Tan, Ping-Heng, Libisch, Florian, and Li, Xiaoqin
- Abstract
In moiré crystals formed by stacking van der Waals materials, surprisingly diverse correlated electronic phases and optical properties can be realized by a subtle change in the twist angle. Here, we discover that phonon spectra are also renormalized in MoS2twisted bilayers, adding an insight to moiré physics. Over a range of small twist angles, the phonon spectra evolve rapidly owing to ultra-strong coupling between different phonon modes and atomic reconstructions of the moiré pattern. We develop a low-energy continuum model for phonons that overcomes the outstanding challenge of calculating the properties of large moiré supercells and successfully captures the essential experimental observations. Remarkably, simple optical spectroscopy experiments can provide information on strain and lattice distortions in moiré crystals with nanometre-size supercells. The model promotes a comprehensive and unified understanding of the structural, optical and electronic properties of moiré superlattices.
- Published
- 2021
- Full Text
- View/download PDF
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