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The effect of physical exercise on erythrocyte 2,3-diphosphoglycerate (2,3-DPG) content was studied in eight trained speed canoeists aged 15–25 years. One-stage 4-min load on a paddling ergometer corresponding to a 1 km race was observed. During the test, the following cardiorespiratory parameters were recorded: . Blood lactate level and haematocrit were also determined. The average resting values of 2,3-DPG and haematocrit were statistically significant increased.