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This article reviews the role of testosterone in normal male sexual anatomic development and function, the consequences of low testosterone on sexual function, and clinical standards for health care providers treating hypogonadal men with sexual dysfunction.

Traditional methods of carbon monitoring in mountainous regions are challenged by complex terrain. Recently, solar‐induced fluorescence (SIF) has been found to be an indicator of gross primary production (GPP), and the increased availability of remotely sensed SIF provides an opportunity to estimate GPP across the Western United States. Although the empirical linkage between SIF and GPP is strong, the current mechanistic understanding of this linkage is incomplete and depends upon changes in leaf biochemical processes in which absorbed sunlight leads to photochemistry, heat (via nonphotochemical quenching [NPQ]), fluorescence, or tissue damage. An improved mechanistic understanding is necessary to leverage SIF observations to improve representation of ecosystem processes within land surface models. Here we included an improved fluorescence model within the Community Land Model, Version 4.5 (CLM 4.5), to simulate seasonal changes in SIF at a subalpine forest in Colorado. We found that when the model accounted for sustained NPQ, this provided a larger seasonal change in fluorescence yield leading to simulated SIF that more closely resembled the observed seasonal pattern (Global Ozone Monitoring Experiment‐2 [GOME‐2] satellite platform and a tower‐mounted spectrometer system). We found that an acclimation model based on mean air temperature was a useful predictor for sustained NPQ. Although light intensity was not an important factor for this analysis, it should be considered before applying the sustained NPQ and SIF to other cold climate evergreen biomes. More leaf‐level fluorescence measurements are necessary to better understand the seasonal relationship between sustained and reversible components of NPQ and to what extent that influences SIF. Sustained nonphotochemical quenching is the major sink for excess light for a high‐elevation conifer forest in winterA temperature‐based acclimation model was able to reproduce seasonal changes in sustained NPQIncluding a representation of sustained NPQ improved the simulation of SIF for cold‐climate evergreens within a land surface model

Bone health declines in the initial years after Roux-en-Y gastric bypass (RYGB), but long-term skeletal effects are unclear.

Mammalian cells can die by apoptosis or by one of several non-apoptotic mechanisms, such as ferroptosis. Here, we present a protocol to distinguish ferroptosis from other cell death mechanisms in cultured cells. We describe steps for seeding cells, administering mechanism-specific cell death inducers and inhibitors, and measuring cell death and viability. We then detail the use of molecular markers to verify mechanisms of cell death. This protocol can be used to identify and distinguish ferroptosis in 2D and 3D cultures.

Allogeneic hematopoietic cell transplantation (HCT) may produce long-term survival in AML after relapse or primary induction failure (PIF). However, outcomes of HCT performed for AML not in remission are historically poor given high relapse rates and transplant-related mortality. Preliminary studies suggest conditioning with clofarabine and myeloablative busulfan (CloBu4) may exert significant anti-leukemic effects without excessive toxicity in refractory hematologic malignancies. A prospective multicenter phase II trial was conducted to determine the efficacy of CloBu4 for patients proceeding directly to HCT with AML not in remission. Seventy-one patients (median age: 56 years) received CloBu4. At day 30 after HCT, 90% achieved morphologic remission. The incidence of non-relapse mortality and relapse at 2 years was 25% and 55%, respectively. The 2-year overall survival (OS) and event-free survival (EFS) were 26% and 20%, respectively. Patients entering HCT in PIF had significantly greater EFS than those in relapse (34% vs 8%; P<0.01). Multivariate analysis comparing CloBu4 with a contemporaneous cohort (Center for International Blood and Marrow Transplantation Research) of AML not in remission receiving other myeloablative conditioning (n=105) demonstrated similar OS (HR: 1.33, 95% confidence interval: 0.92–1.92; P=0.12). HCT with myeloablative CloBu4 is associated with high early response rates and may produce durable remissions in select patients with AML not in remission.

Diffuse large B-cell lymphoma (DLBCL) remains a formidable diagnosis in need of new treatment paradigms. In this work, we elucidated an opportunity for therapeutic synergy in DLBCL by reactivating tumor protein p53 with a stapled peptide, ATSP-7041, thereby priming cells for apoptosis and enhancing their sensitivity to BCL-2 family modulation with a BH3-mimetic, ABT-263 (navitoclax). While this combination was highly effective at activating apoptosis in DLBCL in vitro, it was highly toxic in vivo, resulting in a prohibitively narrow therapeutic window. We, therefore, developed a targeted nanomedicine delivery platform to maintain the therapeutic potency of this combination while minimizing its toxicity via packaging and targeted delivery of a stapled peptide. We developed a CD19-targeted polymersome using block copolymers of poly(ethylene glycol) disulfide linked to poly(propylene sulfide) (PEG-SS-PPS) for ATSP-7041 delivery into DLBCL cells. Intracellular delivery was optimized in vitroand validated in vivoby using an aggressive human DLBCL xenograft model. Targeted delivery of ATSP-7041 unlocked the ability to systemically cotreat with ABT-263, resulting in delayed tumor growth, prolonged survival, and no overt toxicity. This work demonstrates a proof-of-concept for antigen-specific targeting of polymersome nanomedicines, targeted delivery of a stapled peptide in vivo, and synergistic dual intrinsic apoptotic therapy against DLBCL via direct p53 reactivation and BCL-2 family modulation.

Key scientific results from recent experiments, modeling tools, and heavy ion accelerator research are summarized that explore ways to investigate the properties of high energy density matter in heavy-ion-driven targets, in particular, strongly-coupled plasmas at 0.01 to 0.1 times solid density for studies of warm dense matter, which is a frontier area in high energy density physics. Pursuit of these near-term objectives has resulted in many innovations that will ultimately benefit heavy ion inertial fusion energy. These include: neutralized ion beam compression and focusing, which hold the promise of greatly improving the stage between the accelerator and the target chamber in a fusion power plant; and the Pulse Line Ion Accelerator (PLIA), which may lead to compact, low-cost modular linac drivers.

In the present study, 6 mm nominal thickness dissimilar steel plates were joined using friction stir welding. The materials used were duplex stainless steel and low alloy structural steel. The weld was assessed by metallographic examination and mechanical testing (transverse tensile and fatigue). Microstructural examination identified four distinct weld zones and a substantially hard region within the stir zone at the base of the weld tool pin. Fatigue specimens demonstrated high level fatigue life and identified four distinct fracture modes.

In the present study, 6 mm nominal thickness dissimilar steel plates were joined using friction stir welding. The materials used were duplex stainless steel and low alloy structural steel. The weld was assessed by metallographic examination and mechanical testing (transverse tensile and fatigue). Microstructural examination identified four distinct weld zones and a substantially hard region within the stir zone at the base of the weld tool pin. Fatigue specimens demonstrated high level fatigue life and identified four distinct fracture modes.

One of the key components for future space exploration, manned or unmanned, is the availability of propulsion systems beyond the state of the art. The rapid development in conventional propulsion systems since the middle of the 20thcentury has already reached the limits of chemical propulsion technology. To enhance mission radius, shorten the transit time and also extend the lifetime of a spacecraft more efficient, but still powerful propulsion system must be developed. Apart from the propulsion system a major weight contribution arises from the required energy source. Envisioning rapid development of future high average power laser systems and especially the ICAN project we review the prospect of advanced space propulsion based on laser systems.

Modern manufacturing companies are under economic pressure to respond more rapidly to a changing production environment, including short product life cycles, individually customisable products, and the introduction of new disruptive manufacturing processes. This paper details a new manufacturing philosophy and software architecture for Evolvable Assembly Systems, based on the principles of decentralization, context-awareness and intelligent resources, that is implemented using intelligent agent technology and a data distribution service. We also detail the Smart Manufacturing and Reconfigurable Technology demonstrator - an instantiation of the Evolvable Assembly Systems architecture - that implements a proof-of-concept abstract custom pharmaceuticals production line.

The adverse side effects of doxorubicin, including cardiotoxicity and cancer treatment-related fatigue, have been associated with inflammatory cytokines, many of which are regulated by mitogen-activated protein kinases (MAPKs). ZAK is an upstream kinase of the MAPK cascade. Using mouse primary macrophages cultured from ZAK-deficient mice, we demonstrated that ZAK is required for the activation of JNK and p38 MAPK by doxorubicin. Nilotinib, ponatinib and sorafenib strongly suppressed doxorubicin-mediated phosphorylation of JNK and p38 MAPK. In addition, these small molecule kinase inhibitors blocked the expression of IL-1β, IL-6 and CXCL1 RNA and the production of these proteins. Co-administration of nilotinib and doxorubicin to mice decreased the expression of IL-1β RNA in the liver and suppressed the level of IL-6 protein in the serum compared with mice that were injected with doxorubicin alone. Therefore, by reducing the production of inflammatory mediators, the inhibitors identified in the current study may be useful in minimizing the side effects of doxorubicin and potentially other chemotherapeutic drugs.

The Heavy Ion Fusion Science Virtual National Laboratory (HIFS-VNL) is currently developing design concepts for NDCX-II, the second phase of the Neutralized Drift Compression Experiment, which will use ion beams to explore Warm Dense Matter (WDM) and Inertial Fusion Energy (IFE) target hydrodynamics. The ion induction accelerator will consist of a new short pulse injector and induction cells from the decommissioned Advanced Test Accelerator (ATA) at Lawrence Livermore National Laboratory (LLNL). To fit within an existing building and to meet the energy and temporal requirements of various target experiments, an aggressive beam compression and acceleration schedule is planned. WDM physics and ion-driven direct drive hydrodynamics will initially be explored with 30 nC of lithium ions in experiments involving ion deposition, ablation, acceleration and stability of planar targets. Other ion sources which may deliver higher charge per bunch will be explored. A test stand has been built at Lawrence Berkeley National Laboratory (LBNL) to test refurbished ATA induction cells and pulsed power hardware for voltage holding and ability to produce various compression and acceleration waveforms. Another test stand is being used to develop and characterize lithium-doped aluminosilicate ion sources. The first experiments will include heating metallic targets to 10,000 K and hydrodynamics studies with cryogenic hydrogen targets.

Cities are taking the lead on climate change mitigation with ambitious goals to reduce carbon dioxide (CO2) emissions. The implementation of effective mitigation policies will require accurate measurements to guide policy decisions and monitor their efficacy. Here, we present a comprehensive CO2inventory of an urban temperate forest and unmanaged grassland using field observations. We estimate the annual storage of CO2by vegetation and soils and place our biogenic flux estimates in the context of local fossil fuel (FF) emissions to determine when, where, and by how much biogenic fluxes alter net CO2flux dynamics. We compare our hourly estimates of biogenic fluxes in the forest site to modeled estimates using a modified version of Urban‐Vegetation Photosynthesis and Respiration Model (Urban‐VPRM) in Washington DC/Baltimore area presenting the first urban evaluation of this model. We estimate that vegetation results in a net biogenic uptake of −2.62 ± 1.9 Mg C ha−1yr−1in the forest site. FF emissions, however, drive patterns in the net flux resulting in the region being a net source of CO2on daily and annual timescales. In the summer afternoons, however, the net flux is dominated by the uptake of CO2by vegetation. The Urban‐VPRM closely approximates hourly forest inventory based estimates of gross ecosystem exchange but overestimates ecosystem respiration in the dormant season by 40%. Our study highlights the importance of including seasonal dynamics in biogenic CO2fluxes when planning and testing the efficacy of CO2emission reduction polices and development of monitoring programs. The quantification of biogenic carbon dioxide (CO2) fluxes in cities is important to developing, reporting, and testing the efficacy of greenhouse gas emission reduction policies. Yet, to date there is a lack of detailed CO2flux inventories conducted in urban ecosystems which is hindering our abilities to advance and evaluate urban carbon cycling models. Our study characterizes how unique features of urbanization influence carbon cycling dynamics in a temperate forest and grassland and provides a critical evaluation of a model for estimating biogenic fluxes of CO2. We identify key areas of needed model development and identify the seasonal and temporal significance of biogenic fluxes relative to total CO2fluxes (biogenic + anthropogenic sources) in our study area. In urban landscapes, the biogenic carbon dioxide (CO2) fluxes from forest patches and grassland areas vary at fine temporal and spatial scalesThe Urban‐Vegetation Photosynthesis and Respiration Model closely approximated temporal trends in gross ecosystem exchange but overestimated ecosystem respiration in the wintertimeFossil fuel emissions drive patterns in the net flux of CO2except in summer afternoons when biogenic fluxes dominate In urban landscapes, the biogenic carbon dioxide (CO2) fluxes from forest patches and grassland areas vary at fine temporal and spatial scales The Urban‐Vegetation Photosynthesis and Respiration Model closely approximated temporal trends in gross ecosystem exchange but overestimated ecosystem respiration in the wintertime Fossil fuel emissions drive patterns in the net flux of CO2except in summer afternoons when biogenic fluxes dominate

Key scientific results from recent experiments, modeling tools, and heavy ion accelerator research are summarized that explore ways to investigate the properties of high energy density matter in heavy-ion-driven targets, in particular, strongly-coupled plasmas at 0.01 to 0.1 times solid density for studies of warm dense matter, which is a frontier area in high energy density physics. Pursuit of these near-term objectives has resulted in many innovations that will ultimately benefit heavy ion inertial fusion energy. These include: neutralized ion beam compression and focusing, which hold the promise of greatly improving the stage between the accelerator and the target chamber in a fusion power plant; and the Pulse Line Ion Accelerator (PLIA), which may lead to compact, low-cost modular linac drivers.

AbstractCritical issues and ion beam requirements are explored for fast ignition using ion beams to provide fuel compression using indirect drive and to provide separate short-pulse ignition heating using direct drive. Several ion species with different hohlraum geometries are considered for both accelerator-produced and laser-produced ion ignition beams. Ion-driven fast ignition targets are projected to have modestly higher gains than with conventional heavy ion fusion and may offer some other advantages for target fabrication and for use of advanced fuels. However, much more analysis and additional experiments are needed before conclusions can be drawn regarding the feasibility for meeting the ion beam transverse and longitudinal emittances, focal spots, pulse lengths, and target standoff distances required for ion-driven fast ignition.

The acceleration of ions by ultra-intense lasers has attracted great attention due to the unique properties and the unmatched intensities of the ion beams. In the early days the prospects for applications were already studied, and first experiments have identified some of the areas where laser accelerated ions can contribute to the ongoing inertial confinement fusion (ICF) research. In addition to the idea of laser driven proton fast ignition (PFI) and its use as a novel diagnostic tool for radiography the strong dependence on the electron transport in the target was found to be helpful in investigating the energy transport by electrons in fast ignitor scenarios. More recently an additional idea has been presented to use laser accelerated ion beams as the next generation ion sources, and taking advantage of the luminosity of the beams, to develop a test bed for heavy ion beam driven inertial confinement fusion physics. We review our recent experiments and simulations relevant to ICF research presenting a possible scenario for PFI as well as the prospects for next generation ion sources.

Exopolymers are thought to influence bacterial adhesion to surfaces, but the time-dependent nature of molecular-scale interactions of biopolymers with a surface are poorly understood. In this study, the adhesion forces between two proteins and a polysaccharide [Bovine serum albumin (BSA), lysozyme, or dextran] and colloids (uncoated or BSA-coated carboxylated latex microspheres) were analyzed using colloid probe atomic force microscopy (AFM). Increasing the residence time of an uncoated or BSA-coated microsphere on a surface consistently increased the adhesion force measured during retraction of the colloid from the surface, demonstrating the important contribution of polymer rearrangement to increased adhesion force. Increasing the force applied on the colloid (loading force) also increased the adhesion force. For example, at a lower loading force of ~0.6 nN there was little adhesion (less than −0.47 nN) measured between a microsphere and the BSA surface for an exposure time up to 10 s. Increasing the loading force to 5.4 nN increased the adhesion force to −4.1 nN for an uncoated microsphere to a BSA surface and to as much as −7.5 nN for a BSA-coated microsphere to a BSA-coated glass surface for a residence time of 10 s. Adhesion forces between colloids and biopolymer surfaces decreased inversely with pH over a pH range of 4.5−10.6, suggesting that hydrogen bonding and a reduction of electrostatic repulsion were dominant mechanisms of adhesion in lower pH solutions. Larger adhesion forces were observed at low (1 mM) versus high ionic strength (100 mM), consistent with previous AFM findings. These results show the importance of polymers for colloid adhesion to surfaces by demonstrating that adhesion forces increase with applied force and detention time, and that changes in the adhesion forces reflect changes in solution chemistry.

AbstractWe report on an ongoing study on modular Heavy Ion Fusion (HIF) drivers. The modular driver is characterized by ~20 nearly identical induction linacs, each carrying a single high current beam. In this scheme, one of the full size induction linacs can be tested as an “integrated Research Experiment” (IRE). Hence this approach offers significant advantages in terms of driver development path. For beam transport, these modules use solenoids, which are capable of carrying high line charge densities, even at low energies. A new injector concept allows compression of the beam to high line densities right after the source. The final drift compression is performed in a plasma in which the large repulsive space charge effects are neutralized. Finally, the beam is transversely compressed onto the target, using either external solenoids or current-carrying channels (in the assisted pinch mode of beam propagation). We report on progress towards a self-consistent point design from injector to target. Considerations of driver architecture, chamber environment as well as the methodology for meeting target requirements of spot size, pulse shape and symmetry are also described. Finally, some near-term experiments to address the key scientific issues are discussed.

Electrostatic and hydrophobic forces are generally recognized as important in bacterial adhesion. Current continuum models for these forces often wrongly predict measurements of bacterial adhesion forces. The hypothesis tested here is that even qualitative guides to bacterial adhesion often require more than continuum information about hydrophobic forces; they require knowledge about molecular details of the bacteria and substrate surface. In this study, four different strains of bacteria were adsorbed to silica surfaces hydrophobized with alkylsilanes. The thickness of the lipopolysaccharide layers varied on the different bacteria, and the lengths of the alkylsilane molecules were varied from experiment to experiment. Bacterial adhesion was assessed using column experiments and atomic force microscopy (AFM) experiments. Results show that hydrophobized surfaces have higher bacterial sticking coefficients and stronger adhesion forces than bare silica surfaces, as expected. However, adhesion decreased as the solution Debye length became longer than the alkylsilane, perhaps since the silane molecules could not “reach” the bacterial surface. Similarly, those bacteria with a long o-antigen layer had decreased adhesion, perhaps since the silane molecules could not reach surface-bound proteins on the bacteria. This study reveals that macroscopic measurements such as contact angle are not able to fully describe bacterial adhesion; rather, additional details such as the molecular length are required to predict adhesion.

The atomic force microscope (AFM) has been used to examine the stickiness of bacteria on the basis of the analysis of approach and retraction force curves between the AFM tip and the bacterial surface. One difficulty in analyzing approach curve data is that the distance between the AFM tip and the surface of the bacterium is difficult to define. The exact distances are difficult to determine because the surface of the bacterium deforms during force imaging, producing a highly nonlinear region in the approach curve. In this study, AFM approach and retraction curves were obtained using a colloid probe AFM for three strains of Escherichia coli (D21, D21f2, and JM109). These strains differed in their relative adhesion to glass surfaces, on the basis of measurements of sticking coefficients in packed bed flow through column tests. A gradient force curve analysis method was developed to model the interactions between the colloid probe and a surface. Gradient analysis of the approach curve revealed four different regions of colloid−surface interactions during the approach and contact of the probe with the bacterial surface:  a noninteraction region, a noncontact phase, a contact phase, and a constant compliance region. The noncontact phase, which ranged from 28 to 59 nm for the three bacterial strains, was hypothesized to arise primarily from steric repulsion of the colloid by extracellular polymers on the bacterial surface. The contact phase, spanning 59−113 nm, was believed to arise from the initial pressure of the colloid on the outer membrane of the cell. The constant compliance region likely reflected the response of the colloid probe to the stiff peptidoglycan layer that confers strength and rigidity to gram negative bacteria. It was shown that the sticking coefficients reported for the three E. coli strains were correlated with the length of the noncontact phase but not the properties of the other phases. Sticking coefficients were also not correlated with any parameters determined from retraction force curves such as pull-off distances or separation energies. These results show that gradient analysis is useful for studying the contribution of the length of the exopolymers on the cell surface to bacterial adhesion to glass surfaces.

Trifluoromethanesulfonate and tetrafluoroborate salts of the previously in situ-generated synthetic precursor [Tp*W(NCMe)(CO)3]⁺ and a propionitrile analogue, [Tp*W(NCEt)(CO)3](CF3SO3), have been isolated and fully characterized. The complex cations exhibit a carbonyl-capped octahedral structure with Cs symmetry and undergo slow nitrile ligand exchange.

There has been rapid progress in inertial fusion in the past few years. This progress spans the construction of ignition facilities, a wide range of target concepts and the pursuit of integrated programmes to develop fusion energy using lasers, ion beams and z-pinches.Two ignition facilities are under construction, the national ignition facility (NIF) in the United States and the laser megajoule (LMJ) in France, and both projects are progressing towards an initial experimental capability. The laser integration line prototype beamline for LMJ and the first four beams of NIF will be available for experiments in 2003. The full 192 beam capability of NIF will be available in 2009 and ignition experiments are expected to begin shortly after that time.There is steady progress in target science and target fabrication in preparation for indirect-drive ignition experiments on NIF. Advanced target designs may lead to 5–10 times more yield than initial target designs. There has also been excellent progress on the science of ion beam and z-pinch-driven indirect-drive targets.Excellent progress on direct-drive targets has been obtained on the Omega laser at the University of Rochester. This includes improved performance of targets with a pulse shape predicted to result in reduced hydrodynamic instability. Rochester has also obtained encouraging results from initial cryogenic implosions.There is widespread interest in the science of fast ignition because of its potential for achieving higher target gain with lower driver energy and relaxed target fabrication requirements. Researchers from Osaka have achieved outstanding implosion and heating results from the Gekko XII Petawatt facility and implosions suitable for fast ignition have been tested on the Omega laser.A broad-based programme to develop lasers and ion beams for inertial fusion energy (IFE) is under way with excellent progress in drivers, chambers, target fabrication and target injection. KrF and diode pumped solid-state lasers are being developed in conjunction with dry-wall chambers and direct-drive targets. Induction accelerators for heavy ions are being developed in conjunction with thick-liquid protected wall chambers and indirect-drive targets.

AbstractOver the next three years the research program of the Heavy Ion Fusion Virtual National Laboratory (HIF-VNL), a collaboration among LBNL, LLNL, and PPPL, is focused on separate scientific experiments in the injection, transport and focusing of intense heavy ion beams at currents from 100 mA to 1 A. As a next major step in the HIF-VNL program, we aim for a complete “source-to-target” experiment, the Integrated Beam Experiment (IBX). By combining the experience gained in the current separate beam experiments IBX would allow the integrated scientific study of the evolution of a single heavy ion beam at high current (~1 A) through all sections of a possible heavy ion fusion accelerator: the injection, acceleration, compression, and beam focusing.This paper describes the main parameters and technology choices of the planned IBX experiment. IBX will accelerate singly charged potassium or argon ion beams up to 10 MeV final energy and a longitudinal beam compression ratio of 10, resulting in a beam current at target of more than 10 Amperes. Different accelerator cell design options are described in detail: Induction cores incorporating either room temperature pulsed focusing-magnets or superconducting magnets.

AbstractAn updated, self-consistent point design for a heavy ion fusion (HIF) power plant based on an induction linac driver, indirect-drive targets, and a thick liquid wall chamber has been completed. Conservative parameters were selected to allow each design area to meet its functional requirements in a robust manner, and thus this design is referred to as the Robust Point Design (RPD-2002). This paper provides a top-level summary of the major characteristics and design parameters for the target, driver, final focus magnet layout and shielding, chamber, beam propagation to the target, and overall power plant.

The influence of the lipopolysaccharide (LPS) chain length on bacterial adhesion was investigated by measuring the collision efficiencies of three Escherichia coli K12 strains, each having a different length LPS, to silica glass beads in column tests (macroscale tests). Nanoscale interactions between the bacteria and a silicon nitride tip were probed utilizing atomic force microscopy (AFM). Adhesion results based on column tests indicated that collision efficiencies of the three bacteria were not consistently correlated to LPS length. Under conditions of low ionic strength (1 mM NaCl), collision efficiencies increased with LPS length for the three strains of E. coli. However, if cells were fixed with glutaraldehyde (2.5%), the strain with the shortest LPS chain had the greatest adhesion, while the bacterium with the mid-length LPS had the least adhesion to glass beads. Collision efficiencies increased when the solution ionic strength was increased from 1 to 100 mM as expected, and in most cases glutaraldehyde treatment also increased adhesion. AFM force curves failed to distinguish the adhesion characteristics of these bacteria measured in column tests, as all AFM force curves on the bacteria were identical. Changes in adhesion were also not predictable by more conventional measurements of bacterial properties based on ζ potential or contact angle. These results suggest that the LPS molecule length is not the sole determinant of adhesion of the three E. coli strains in porous media and that AFM force curve analysis, zeta potential, or contact angle data cannot yet be used to fully predict adhesion of these three strains to glass beads.

Atomic force microscopy (AFM) has become an important tool for investigating various biological materials, and it is now being applied more routinely for imaging bacteria. By imaging bacteria in water, AFM can provide in-situ images of viable cells and be used to measure interaction forces between the AFM tip (or a colloid probe) and the cell surface. However, the relatively large height and compliance of the bacterium can also lead to imaging artifacts. AFM images of Escherichia coli K12 were consistently found to contain image shadows that were oriented in parallel lines 27° from the direction of the cantilever tilt, regardless of the scan direction. Similar image shadows were also observed for 1 μm diameter polystyrene latex microspheres. Using a simple geometric model for the interaction of the tip and the bacterium, it is demonstrated here that these lines observed for bacteria are image artifacts produced by the pyramidal shape of the tip, the 10° tilt of the cantilever, and the height of the bacterium relative to the size of the tip. Such image artifacts disappear when we image dehydrated bacteria that are lower in height, or bacteria that become damaged and deflated during imaging in water. The interaction of the edge of the tip with the bacterium is also shown to result in inconsistent shapes of force curves unless the force curve is centered on the crest of the rounded bacterial surface.

Highly ionized plasmas are being considered as a medium for charge neutralizing heavy ion beams in order to focus beyond the space-charge limit. Calculations suggest that plasma at a density of 1–100 times the ion beam density and at a length ~0.1–2 m would be suitable for achieving a high level of charge neutralization. An Electron Cyclotron Resonance (ECR) source has been built at the Princeton Plasma Physics Laboratory (PPPL) to support a joint Neutralized Transport Experiment (NTX) at the Lawrence Berkeley National Laboratory (LBNL) to study ion beam neutralization with plasma. The ECR source operates at 13.6 MHz and with solenoid magnetic fields of 1–10 gauss. The goal is to operate the source at pressures ~10−6 Torr at full ionization. The initial operation of the source has been at pressures of 10−4–10−1 Torr. Electron densities in the range of 108 to 1011 cm−3 have been achieved. Low-pressure operation is important to reduce ion beam ionization. A cusp magnetic field has been installed to improve radial confinement and reduce the field strength on the beam axis. In addition, axial confinement is believed to be important to achieve lower-pressure operation. To further improve breakdown at low pressure, a weak electron source will be placed near the end of the ECR source. This article also describes the wave damping mechanisms. At moderate pressures (> 1 mTorr), the wave damping is collisional, and at low pressures (&lt; 1 mTorr) there is a distinct electron cyclotron resonance.

Abstract: We studied the biochemical composition and photosynthetic characteristics of the aerial parasite eastern dwarf mistletoe (Arceuthobium pusillum)and the effect of infection on the needles of host white spruce (Picea glauca)in a coastal forest stand in Maine, USA. Eastern dwarf mistletoe was capable of photosynthetic oxygen evolution; however, rates were low and were exceeded by respiratory oxygen consumption at all light intensities through full sunlight. Therefore, eastern dwarf mistletoe acts as a net sink for host photosynthate. Relative to those of uninfected trees, needles from infected branches of white spruce were significantly smaller in terms of length, fresh weight, maximum cross section and the diameter of the vascular cylinder. Needles of uninfected and infected trees did not differ in terms of fresh weight to dry weight ratio, nor in nitrogen, soluble sugar or starch content. Needles of infected trees possessed significantly less α‐carotene and neoxanthin, but did not otherwise differ from uninfected needles in terms of chlorophyll and carotenoid composition. Since specific physiological roles for α‐carotene and neoxanthin have not been described, the functional significance of the decreases in their content is not known. Photosynthetic capacities of needles from infected and uninfected white spruce did not differ significantly, as measured by oxygen evolution. These findings suggest that dwarf mistletoe infection does not substantially perturb host white spruce source‐sink balance at the end of the growing season and that carbon exchange dynamics between the host and parasite are unlikely to fully explain the detrimental effects of infection on white spruce.

Probing nanoscale interactions between an atomic force microscope tip and a bacterium may provide insight into the molecular level origins of bacterial adhesion. Distinguishing between the relevant surface interaction forces, such as steric and electrostatic interactions, is complicated by the elastic deformation of the bacterium. To probe the possible role of lipopolysaccharides (LPS) in bacterial adhesion and cell elasticity, atomic force microscopy (AFM) force images were obtained between a bare silicon nitride AFM tip and three different Escherichia coli K12 strains, each having a different length of LPS on their surface. Cell elasticity was varied with glutaraldehyde fixation. Bacterial force curves consisted of a nonlinear region 20−30 nm above the cell surface and a constant compliance (linear region) with a slope that was significantly less than that of a hard surface. AFM force curves obtained on the top of the cell were identical (linear and nonlinear regions) for all three strains, indicating a lack of a steric contribution of LPS to the force curve. Force images obtained off the center of the cell produced apparent long-range forces, but these were considered to be imaging artifacts produced by tip−surface geometries at the cell edges. Glutaraldehyde strongly affected the elasticity of the cell but did not affect the nonlinear portion of the force curve. The effective spring constant of the bacterium, calculated from the constant compliance region of the force curve, was found to increase 4-fold with the addition of 2.5% glutaraldehyde and was independent of the spring constant of the cantilever. The nonlinear portion of the curve is not consistent with electrostatic forces, because interaction distances were not a function of solution ionic strength. These results suggested that nonlinear forces were due to deformation of the bacterial surface layer.

This is the final report of a panel set up by the U.S. Department of Energy (DOE) Fusion Energy Sciences Advisory Committee (FESAC) in response to a charge letter dated September 10, 2002 from Dr. Ray Orbach, Director of the DOE's Office of Science. In that letter, Dr. Orbach asked FESAC to develop a plan with the end goal of the start of operation of a demonstration power plant in approximately 35 years. This report, submitted March 5, 2003, presents such a plan, leading to commercial application of fusion energy by mid-century. The plan is derived from the necessary features of a demonstration fusion power plant and from the time scale defined by President Bush. It identifies critical milestones, key decision points, needed major facilities and required budgets. The report also responds to a request from DOE to FESAC to describe what new or upgraded fusion facilities will “best serve our purposes” over a time frame of the next twenty years.

We studied the biochemical composition and photosynthetic characteristics of the aerial parasite eastern dwarf mistletoe (Arceuthobium pusillum) and the effect of infection on the needles of host white spruce (Picea glauca) in a coastal forest stand in Maine, USA. Eastern dwarf mistletoe was capable of photosynthetic oxygen evolution; however, rates were low and were exceeded by respiratory oxygen consumption at all light intensities through full sunlight. Therefore, eastern dwarf mistletoe acts as a net sink for host photosynthate. Relative to those of uninfected trees, needles from infected branches of white spruce were significantly smaller in terms of length, fresh weight, maximum cross section and the diameter of the vascular cylinder. Needles of uninfected and infected trees did not differ in terms of fresh weight to dry weight ratio, nor in nitrogen, soluble sugar or starch content. Needles of infected trees possessed significantly less α-carotene and neoxanthin, but did not otherwise differ from uninfected needles in terms of chlorophyll and carotenoid composition. Since specific physiological roles for α-carotene and neoxanthin have not been described, the functional significance of the decreases in their content is not known. Photosynthetic capacities of needles from infected and uninfected white spruce did not differ significantly, as measured by oxygen evolution. These findings suggest that dwarf mistletoe infection does not substantially perturb host white spruce source-sink balance at the end of the growing season and that carbon exchange dynamics between the host and parasite are unlikely to fully explain the detrimental effects of infection on white spruce.

The Innovative Confinement Concepts Workshop, ICC2002, provided a forum for presentations and exchange of ideas on the science and status of innovative concepts in the U.S. Fusion Energy Program. The workshop, held at the University of Maryland on January 22–24, 2002, included oral presentations addressing the important science and status of the concepts, posters focussed on details of the work, a skunkworks for novel ideas, and breakout sessions preparing for the July 2002 fusion energy Snowmass meeting. This report summarizes the oral presentations. A web site (https://wormhole.ucllnl.org/ICC2002/) has been established with the abstracts and many of the presentations, both oral and poster, from the workshop.