33 results on '"Luo, Lijuan"'
Search Results
2. The potential beneficial effects of Lactobacillus plantarumGM11 on rats with chronic unpredictable mild stress- induced depression
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Ma, Jie, Wang, Junrui, Wang, Gang, Wan, Yujun, Li, Nanzhen, Luo, Lijuan, Gou, Hongmei, and Gu, Jianwen
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ABSTRACTObjectiveThe main purpose of the present study was to assess the beneficial effect of Lactobacillus plantarumGM11 (LacPGM11), screened from Sichuan traditional fermented food, in depressive rats induced by chronic unpredictable mild stress (CUMS).MethodsMale SPF SD rats were randomly assigned to 3 groups: the control group, CUMS group and CUMS + LacPGM11 group (n = 10). The rats in the CUMS and LacPGM11 groups received CUMS stimulation for 42 d. The behavioral tests and levels of monoamine neurotransmitter, glucocorticoid hormone and brain-derived neurotrophic factor (BDNF) in the serum and hippocampus were measured. The effects of LacPGM11 on the mRNA and protein expression of BDNF and cAMP response element binding protein (CREB) in the hippocampus were also investigated.ResultsAfter supplementation for 21 d, LacPGM11 was associated with alleviation of depressive-like behavior, not anxiety-like behavior, in depressive rats. LacPGM11 increased the levels of 5-hydroxytryptamine (5-HT) and BDNF and decreased the level of cortisol (CORT) in the serum and hippocampus in depressed rats. In addition, treatment with LacPGM11 also increased the mRNA and protein expression of BDNF and CREB in the hippocampus.ConclusionsThis work has revealed that LacPGM11 has potential beneficial effects on depression. This effect might be related to alleviating monoamine neurotransmitter deficiency, HPA axis hyperfunction and CREB-BDNF signaling pathway downregulation. This study demonstrates that LacPGM11 could be a potential therapeutic approach to treat depression and other mental health problems.
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- 2024
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3. Enhancing 3D DNA Walker-Induced CRISPR/Cas12a Technology for Highly Sensitive Detection of ExomicroRNA Associated with Osteoporosis.
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Luo, Lijuan, Dong, Fang, Li, Dandan, Li, Xinmin, Li, Xinyu, Fan, Yunpeng, Qi, Caihong, Luo, Jinyong, Li, Li, and Shen, Bo
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- 2024
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4. Bayesian Estimation of Inverted Beta Mixture Models With Extended Stochastic Variational Inference for Positive Vector Classification
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Lai, Yuping, Guan, Wenbo, Luo, Lijuan, Guo, Yanhui, Song, Heping, and Meng, Hongying
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The finite inverted beta mixture model (IBMM) has been proven to be efficient in modeling positive vectors. Under the traditional variational inference framework, the critical challenge in Bayesian estimation of the IBMM is that the computational cost of performing inference with large datasets is prohibitively expensive, which often limits the use of Bayesian approaches to small datasets. An efficient alternative provided by the recently proposed stochastic variational inference (SVI) framework allows for efficient inference on large datasets. Nevertheless, when using the SVI framework to address the non-Gaussian statistical models, the evidence lower bound (ELBO) cannot be explicitly calculated due to the intractable moment computation. Therefore, the algorithm under the SVI framework cannot directly use stochastic optimization to optimize the ELBO, and an analytically tractable solution cannot be derived. To address this problem, we propose an extended version of the SVI framework with more flexibility, namely, the extended SVI (ESVI) framework. This framework can be used in many non-Gaussian statistical models. First, some approximation strategies are applied to further lower the ELBO to avoid intractable moment calculations. Then, stochastic optimization with noisy natural gradients is used to optimize the lower bound. The excellent performance and effectiveness of the proposed method are verified in real data evaluation.
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- 2024
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5. Personnel Trajectory Extraction From Port-Like Videos Under Varied Rainy Interferences
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Chen, Xinqiang, Wei, Chenxin, Yang, Yang, Luo, Lijuan, Biancardo, Salvatore Antonio, and Mei, Xiaojun
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Large-scale deployed cameras in the automated container terminal (ACT) area helps on-site staff better identify unexpected yet emergency events by monitoring port personnel trajectories. Rainy weather is
${a}$ ${\mathbf {IDF}}_{\mathbf {1}}$ - Published
- 2024
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6. High-throughput fitness experiments reveal specific vulnerabilities of human-adapted Salmonelladuring stress and infection
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Wang, Benjamin X., Leshchiner, Dmitry, Luo, Lijuan, Tuncel, Miles, Hokamp, Karsten, Hinton, Jay C. D., and Monack, Denise M.
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Salmonella entericais comprised of genetically distinct ‘serovars’ that together provide an intriguing model for exploring the genetic basis of pathogen evolution. Although the genomes of numerous Salmonellaisolates with broad variations in host range and human disease manifestations have been sequenced, the functional links between genetic and phenotypic differences among these serovars remain poorly understood. Here, we conduct high-throughput functional genomics on both generalist (Typhimurium) and human-restricted (Typhi and Paratyphi A) Salmonellaat unprecedented scale in the study of this enteric pathogen. Using a comprehensive systems biology approach, we identify gene networks with serovar-specific fitness effects across 25 host-associated stresses encountered at key stages of human infection. By experimentally perturbing these networks, we characterize previously undescribed pseudogenes in human-adapted Salmonella. Overall, this work highlights specific vulnerabilities encoded within human-restricted Salmonellathat are linked to the degradation of their genomes, shedding light into the evolution of this enteric pathogen.
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- 2024
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7. Nanospray Laser-Induced Plasma Ionization Mass Spectrometry for Rapid and Sensitive Analysis of Polycyclic Aromatic Hydrocarbons and Halogenated Derivatives.
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Deng, Jiewei, Xie, Jialiang, Lu, Qiao, Xiao, Xu, Wu, Yuehua, Liu, Ning, Luo, Lijuan, Luan, Tiangang, and Yang, Yunyun
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- 2023
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8. Removal of estrogens from primary settled sewage by repeated culture of Selenastrum capricornutum
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Wang, Ping, Luan, Jianwen, and Luo, Lijuan
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- 2023
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9. Nanospray Laser-Induced Plasma Ionization Mass Spectrometry for Rapid and Sensitive Analysis of Polycyclic Aromatic Hydrocarbons and Halogenated Derivatives
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Deng, Jiewei, Xie, Jialiang, Lu, Qiao, Xiao, Xu, Wu, Yuehua, Liu, Ning, Luo, Lijuan, Luan, Tiangang, and Yang, Yunyun
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Polycyclic aromatic hydrocarbons (PAHs) and halogenated derivatives are a series of environmental pollutants with potential toxicity and health risks on biosystems and the ecosystem. Rapid and sensitive analysis of trace PAHs and halogenated PAHs in complex environmental samples is a challenging topic for analytical science. Here we report the development of a nanospray laser-induced plasma ionization MS method for rapid and sensitive analysis of trace PAHs and halogenated PAHs under ambient and open-air conditions. A nanospray tip was applied for loading samples and placed pointing to the MS inlet, being a nanospray emitter with the application of a high voltage. A beam of laser was focused to induce energetic plasma between the nanospray emitter and the MS inlet for ionization of PAHs and halogenated PAHs for mass spectrometric analysis. Meanwhile, an inner-wall naphthyl-coated nanospray emitter was developed and applied as a solid-phase microextraction (SPME) probe for highly selective enrichment of trace PAHs and halogenated PAHs in complex environmental samples, and some organic solvent was applied to desorb the analytes for nanospray laser-induced plasma ionization MS analysis. Satisfactory linearity for each target PAH and halogenated PAH was obtained, with correlation coefficient values (r) no less than 0.9917. The method showed extremely high sensitivity for analysis of trace PAHs and halogenated PAHs in water, with limits of detection (LODs) and quantification (LOQs) of 0.0001–0.02 and 0.0003–0.08 μg/L, respectively. By using the inner-wall naphthyl-coated nanospray laser-induced plasma ionization MS method, sensitive detection of trace PAHs and halogenated PAHs in real sewage and wastewater samples was successfully achieved.
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- 2023
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10. Visual information and appearance: The impact of visual attributes of user-generated photos on review helpfulness.
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Luo, Lijuan, Liu, Ling, Zheng, Yujie, and Chen, Jing
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ELABORATION likelihood model ,AESTHETICS ,DEEP learning ,FOOD writers ,RESTAURATEURS - Abstract
• We examine the role of UGPs' visual information and appearance on review helpfulness. • We use deep learning to calculate the breadth and depth of photos. • We show visual breadth and depth positively impact on review helpfulness. • Reviewer status and review length moderate these effects. Images have become integral to consumers' sharing of consumption experiences due to their abilities of carrying rich and vivid information. Drawing from the perspective of information theory and the elaboration likelihood model (ELM), this study investigates impacts of visual information and visual appearance of user-generated photos (UGPs) on customers' perceived review helpfulness. We utilize deep learning techniques to calculate the breadth and depth of photos (visual information), and evaluate the aesthetic value of the photos (visual appearance). By collecting a substantial amount of review, reviewer and restaurant information from the Yelp platform, our results demonstrate that the visual breadth and visual depth of review photos have a significant positive impact on review helpfulness. Notably, reviewer status and review length moderate these effects. These insights offer valuable strategies for both restaurant managers and online restaurant platforms regarding UGP management. [ABSTRACT FROM AUTHOR]
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- 2024
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11. Cyclovirobuxine D inhibits the growth of osteosarcoma cells through the induction of autophagy flux arrest by promoting lysosomal acidification.
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Liang, Shiqiong, Xie, Liping, Li, Ziyun, Lu, Qiuping, Zhang, Lulu, Wang, Jiayu, Xia, Haichao, Luo, Lijuan, Wang, Xiaoxuan, and Luo, Jinyong
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[Display omitted] • CVB-D exerted inhibitory effect on osteosarcoma cells in vivo and in vitro. • PI3K-AKT-TOR signaling pathway might be involved in the anti-OS process. • CVB-D initiated autophagy, but impaired autophagy flux. • CVB-D over-acidified lysosomes and inhibited CTSD maturing by binding to ATP6V0A1. Osteosarcoma (OS) is an aggressive primary tumor with the highest incidence in children and adolescents. Natural plant compounds (NPCs) have long been promising resources in the field of antitumor drug discovery because of their high efficacy and low toxicity. Here, the aim of this study is to investigate the potential inhibitory effects of Cyclovirobuxine D (CVB-D), a natural bioactive isolated from the traditional Chinese medicinal herb Huangyang, on OS cells. We showed that CVB-D reduced OS cell growth in vitro and in vivo. Mechanistically, CVB-D inhibited PI3K-AKT-mTOR pathway and initiated autophagy. On the other hand, CVB-D induced lysosomal over-acidification by interacting with the V-type proton ATPase 116 kDa subunit a1 (ATP6V0A1), ultimately leading to autophagy flux arrest which might be related to the inhibitory effect of CVB-D on OS cells. Conclusively, our results propose a potential foundation for CVB-D to be developed into an anti-OS drug and an autophagy inhibitor. [ABSTRACT FROM AUTHOR]
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- 2024
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12. Fecal microbiota transplantation before hematopoietic stem cell transplantation in a pediatric case of chronic diarrhea with a FOXP3 mutation.
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Wu, Wenyan, Shen, Nan, Luo, Lijuan, Deng, Zhaohui, Chen, Jing, Tao, Yue, Mo, Xi, and Cao, Qing
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HEMATOPOIETIC stem cell transplantation ,FECAL microbiota transplantation ,FORKHEAD transcription factors ,DIARRHEA ,BACTERIAL diversity ,FAILURE to thrive syndrome - Abstract
Immune dysregulation, polyendocrinopathy, enteropathy, X-linked (IPEX) syndrome is a rare disorder caused by mutation of the forkhead box protein 3 (FOXP3) gene, often leading to intractable and life-threatening diarrhea. Fecal microbiota transplantation (FMT), has been regarded in recent years as an available approach to reconstruct disrupted gut microbiome and successfully used to attenuates diarrhea induced by different underlying diseases. Therefore, FMT may have curative potential on the symptoms of enteropathy in patients with IPEX syndrome. Physical and laboratory examinations were performed, and clinical data were collected. FMT was administered via frozen fecal microbial solution, and the fecal microbiota composition was analyzed using 16S rDNA sequencing before and after FMT. The patient was diagnosed with IPEX syndrome with a mutation detected in the FOXP3 gene, which was identified as c.767T > C (p.M256T). He presented with recurrent watery diarrhea and respiratory infections after birth and developed a significant failure to thrive. Disturbances in the gut microbiota composition and marked decreased bacterial diversity were observed to be involved in the persistent and refractory diarrhea. After receiving FMT treatment, the patient responded with remission of the diarrhea without apparent side effects. His stool output significantly decreased, corresponding to increased microbial diversity and modification of his microbiota composition. The patient finally achieved full recovery after hematopoietic stem cell transplantation (HSCT). Our data suggest an association between the gut microbiota and clinical symptoms of patient with IPEX syndrome and demonstrate FMT as an alternative therapy for severe diarrhea unresponsive to routine therapy in these patients. [ABSTRACT FROM AUTHOR]
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- 2021
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13. Chlorinated Anthracenes Induced Pulmonary Immunotoxicity in 3D Coculture Spheroids Simulating the Lung Microenvironment
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Li, Xinyan, Zhou, Yiluan, Luo, Lijuan, Zheng, Shuang, Deng, Jiewei, and Luan, Tiangang
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Chlorinated anthracenes (Cl-Ants), persistent organic pollutants, are widely detected in the environment, posing potential lung toxicity risks due to frequent respiratory exposure. However, direct evidence and a comprehensive understanding of their toxicity mechanisms are lacking. Building on our prior findings of Cl-Ants’ immunotoxic risks, this study developed a three-dimensional coculture spheroid model mimicking the lung’s immune microenvironment. The objective is to explore the pulmonary immunotoxicity and comprehend its mechanisms, taking into account the heightened immune reactivity and frequent lung exposure of Cl-Ants. The results demonstrated that Cl-Ants exposure led to reduced spheroid size, increased macrophage migration outward, lowered cell viability, elevated 8-OHdG levels, disturbed anti-infection balance, and altered cytokine production. Specifically, the chlorine substituent number correlates with the extent of disruption of spheroid indicators caused by Cl-Ants, with stronger immunotoxic effects observed in dichlorinated Ant compared to those in monochlorinated Ant. Furthermore, we identified critical regulatory genes associated with cell viability (ALDOC and ALDOA), bacterial response (TLR5 and MAP2K6), and GM-CSF production (CEBPB). Overall, this study offers initial in vitro evidence of low-dose Cl-PAHs’ pulmonary immunotoxicity, advancing the understanding of Cl-Ants’ structure-related toxicity and improving external toxicity assessment methods for environmental pollutants, which holds significance for future monitoring and evaluation.
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- 2024
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14. Natural Porphyrins Accelerating the Phototransformation of Benzo[a]pyrene in Water
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Luo, Lijuan, Xiao, Zhengyu, Chen, Baowei, Cai, Fengshan, Fang, Ling, Lin, Li, and Luan, Tiangang
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Phototransformation is one of the most important transformation pathways of organic contaminants in the water environment. However, how active compounds enable and accelerate the phototransformation of organic pollutants remains to be elucidated. In this study, the phototransformation of benzo[a]pyrene (BaP, the first class “human carcinogens”) by various natural porphyrins under solar irradiation was investigated, including chlorophyll a, sodium copper chlorophyllin, hematin, cobalamin, and pheophorbide a. Transformation efficiency of BaP varied considerably with chemical stabilities of the porphyrins. Porphyrins with a lower stability displayed higher BaP transformation efficiencies. BaP transformation had a significant positive correlation with the production of singlet oxygen. Identical phototransformation products of BaP were observed for all investigated porphyrins, and the main products were identified as BaP-quinones, including BaP-1,6-dione, BaP-3,6-dione, and BaP-6,12-dione. The mechanism of natural porphyrins accelerating the BaP phototransformation in water was proposed to proceed via the photocatalytic generation of singlet oxygen resulting in the transformation of BaP to quinones.
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- 2024
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15. Endovascular treatment of complex middle cerebral artery aneurysms using TuBridge flow diverters
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Liang, Feng, Yang, Yibing, Luo, Lijuan, Liao, Bingye, Zhang, Guofeng, Ou, Siqi, Xiao, Weiping, Guo, Ning, and Qi, Tiewei
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Background The safety and efficacy of the TuBridge flow diverter in treating middle cerebral artery aneurysms remains unknown. In this study, we report our preliminary experience treating complex middle cerebral artery aneurysms using the TuBridge flow diverter.Methods A prospectively maintained database of intracranial aneurysms treated with the TuBridge flow diverter was retrospectively reviewed, and patients with middle cerebral artery aneurysms were included in this study. Demographics, aneurysm features, complications, and clinical and angiographic outcomes were assessed. Evaluation of the angiographic results included occlusion grade of aneurysm (O’Kelly–Marotta grading scale), patency of jailed branch(es), and in-stent stenosis.Results Eight patients with eight middle cerebral artery aneurysms were included in this study. The mean aneurysm size was 11.8 ± 6.8 mm. There were no procedure-related complications and there was no morbidity or mortality at a mean follow-up of 11.3 ± 3.6 months. All patients had follow-up angiograms at a mean of 7.5 ± 4.0 months after surgery. Of the eight patients, there was 1 (12.5%) O’Kelly–Marotta grading scale A, 3 (37.5%) O’Kelly–Marotta grading scale B, 1 (12.5%) O’Kelly–Marotta grading scale C, and 3 (37.5%) O’Kelly–Marotta grading scale D. Of the seven patients with jailed branch, the blood flow of jailed branch was unchanged in 4 (57.1%), decreased in 2 (28.6%), and occluded in 1 (14.3%). In-stent stenosis was mild in 2 (25%) patients and moderate in 1 (12.5%) patient.Conclusion Midterm results suggest that endovascular treatment of middle cerebral artery aneurysms using the TuBridge flow diverter is safe and associated with good outcomes. The TuBridge flow diverter may be an option for complex middle cerebral artery aneurysms that are difficult to treat with either clipping or coiling.
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- 2020
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16. Feasibility of mean platelet volume as a biomarker for chronic obstructive pulmonary disease: A systematic review and meta-analysis
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Ma, Yiming, Zong, Dandan, Zhan, Zijie, Li, Herui, Dai, Zhongshang, Cui, Yanan, Luo, Lijuan, Zeng, Zihang, He, Chenjie, and Chen, Yan
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Objective To evaluate the feasibility of mean platelet volume (MPV) as a biomarker for chronic obstructive pulmonary disease (COPD).Methods A systematic search for studies published up to March 2019 was performed in the PubMed and Web of Science databases. Three independent investigators screened the titles and abstracts of including studies according to eligibility criteria. The Newcastle–Ottawa Scale was used to assess the quality of eligible studies, and statistical analyses were performed using Review Manager version 5.3.Results A total of eight studies with 1230 COPD patients and 443 healthy controls were included in our meta-analysis. No significant differences in MPV level were identified in pairwise comparisons of the acute exacerbations of COPD (AECOPD), stable COPD, and control groups. Furthermore, no significant correlation was observed between MPV level and systemic inflammatory biomarkers.Conclusions MPV does not appear to represent a suitable biomarker of disease phase or inflammatory burden in COPD. However, future large-scale studies should be performed to further investigate the relationship between MPV and COPD.
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- 2019
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17. Natural Porphyrins Accelerating the Phototransformation of Benzo[a]pyrene in Water.
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Luo, Lijuan, Xiao, Zhengyu, Chen, Baowei, Cai, Fengshan, Fang, Ling, Lin, Li, and Luan, Tiangang
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- 2018
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18. Inactivation of Soybean Bowman–Birk Inhibitor by Stevioside: Interaction Studies and Application to Soymilk
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Liu, Chun, Luo, Lijuan, Wu, Ying, Yang, Xiaoquan, Dong, Jie, Luo, Feijun, Zou, Yuan, Shen, Yingbin, and Lin, Qinlu
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In this work, the interaction of the soybean Bowman–Birk inhibitor (BBI) with stevioside (STE) was studied by stopped-flow–fluorescence and molecular docking. STE’s inactivation of protease-inhibitor activities in soymilk and the influence of STE addition on the sensory character of soymilk were also evaluated. The results indicate that STE binds BBI with a binding constant (Ka) of 3.38 × 105L mol–1to form a 1:1 complex. The docking study reveals that two hydrogen bonds are formed between the side-chain of Lys16 (reactive site 1) of BBI and the glucose-ring hydroxyl groups of STE, which may block BBI from contacting trypsin and thus deactivate the trypsin-inhibitor activity (TIA) of BBI. Moreover, the residual TIA in soymilk could also be inactivated by STE. A mixture of 159 mg/L STE and 60 g/L sucrose could be used for sweetening soymilk without affecting the sensory characteristics when compared to a reference product sweetened with 9% sucrose.
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- 2019
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19. Degradation pathways of 1-methylphenanthrene in bacterial Sphingobium sp. MP9-4 isolated from petroleum-contaminated soil.
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Zhong, Jianan, Luo, Lijuan, Chen, Baowei, Sha, Sha, Qing, Qing, Tam, Nora F.Y., Zhang, Yong, and Luan, Tiangang
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MARINE pollution ,OIL pollution of the sea ,SOIL pollution ,POLYCYCLIC aromatic hydrocarbons ,MASS spectrometers - Abstract
Alkylated polycyclic aromatic hydrocarbons (PAHs) are abundant in petroleum, and alkylated phenanthrenes are considered as the primary PAHs during some oil spill events. Bacterial strain of Sphingobium sp. MP9-4, isolated from petroleum-contaminated soil, was efficient to degrade 1-methylphenanthrene (1-MP). A detailed metabolism map of 1-MP in this strain was delineated based on analysis of metabolites with gas chromatograph–mass spectrometer (GC–MS). 1-MP was initially oxidized via two different biochemical strategies, including benzene ring and methyl-group attacks. Benzene ring attack was initiated with dioxygenation of the non-methylated aromatic ring via similar degradation pathways of phenanthrene (PHE) by bacteria. For methyl-group attack, mono oxygenase system was involved and more diverse enzymes were needed than that of PHE degradation. This study enhances the understanding of the metabolic pathways of alkylated PAHs and shows the significant potential of Sphingobium sp. MP9-4 for the bioremediation of alkylated PAHs contaminated environments. [ABSTRACT FROM AUTHOR]
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- 2017
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20. Development of a Novel ListeriaEnrichment Broth for the Isolation of Pathogenic Listeria
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Liu, Dongxin, Wang, Yan, Wang, Yi, Zhang, Lu, Luo, Lijuan, Liu, Kai, and Ye, Changyun
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Listeriosis, the disease caused by pathogenic Listeriaspecies, can present severe symptoms in susceptible people. The goal of this study was to develop a novel enrichment broth, Listeriaallose enrichment broth (LAEB), to improve isolation of Listeria monocytogenesand Listeria ivanoviifrom samples through incorporating a specific carbohydrate and reducing inhibitor concentrations. Other coexisting bacteria, particularly Listeria innocua,can interfere with the isolation of pathogenic Listeriain such ways as overgrowth of L. innocuaand the generation of inhibitory metabolites. The incorporation of allose into the novel LAEB was effective for slowing the growth of L. innocuaand other nontarget microorganisms. We determined that 35°C and pH 7.0 under aerobic conditions are optimal for Listeriagrowth in this medium. The novelty of the use of LAEB is the single enrichment procedure at 35°C for 24 h, obviating the need for a secondary enrichment medium. In 50 simulated samples, the sensitivity of the LAEB method (86%) was higher than that of the International Organization for Standardization (EN ISO) method (70%). In 142 naturally contaminated samples tested, the isolation rate for pathogenic Listeriawith the LAEB method was 26.0% (37 of 142 samples), which was significantly higher than the 17.6% (25 of 142 samples) for the EN ISO method. Higher isolation rates and a quicker and easier protocol make the novel LAEB method an appropriate alternative for the isolation of pathogenic Listeria.
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- 2017
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21. Neferine inhibits the growth of human osteosarcoma cells through activating P38/JNK and suppressing Wnt/β-catenin signaling pathway.
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Xu, Jingtao, Wang, Xiaoxuan, Luo, Lijuan, Xia, Haichao, Lu, Qiuping, Huang, Yanran, Wang, Yuping, Zhang, Jun, Jiwa, Habu, Zhao, Runhan, Xiong, Chuang, Luo, Jinyong, and Luo, Xiaoji
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• Neferine is a Chinese traditional medicine, has shown inhibitory effects in osteosarcoma. • Evaluate the inhibitory effects of Neferine in vivo and vitro. • Inhibiting tumor growth by Wnt/β-catenin and P38/JNK pathway. • Find an efficient and secure solution for osteosarcoma. Osteosarcoma (OS) is one of the most common types of bone malignant tumors. Clinical commonly used therapeutic drugs of OS treatment are prone to toxic and side effects, so it is supposed to develop new drugs with low toxicity and low side effects. As a Chinese traditional medicine, Neferine(C38H44N2O6) has inhibitory effects in various tumors. In the present study, we investigated the effects of Nef on OS cells in vitro and in vivo. We found that Nef inhibited cell proliferation, reduced migration, decreased invasion, and induced cell cycle arrest of OS cells. Notably, we demonstrated that Nef had no obvious effect on proliferation and apoptosis of normal cells. Besides, Nef repressed tumor growth of OS cells in xenograft mouse model. Mechanically, we found that Nef increased the phosphorylation level of P38 and JNK and suppressed Wnt/β-Catenin signaling pathway. In summary, our research validates that Nef may inhibit the proliferation, migration, and invasion of OS and promote apoptosis possibly by activating P38/JNK and suppressing Wnt/β-Catenin signaling pathway. [ABSTRACT FROM AUTHOR]
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- 2022
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22. Sulfonated Holey Graphene Oxide (SHGO) Filled Sulfonated Poly(ether ether ketone) Membrane: The Role of Holes in the SHGO in Improving Its Performance as Proton Exchange Membrane for Direct Methanol Fuel Cells
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Jiang, Zhong-Jie, Jiang, Zhongqing, Tian, Xiaoning, Luo, Lijuan, and Liu, Meilin
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Sulfonated holey graphene oxides (SHGOs) have been synthesized by the etching of sulfonated graphene oxides with concentrated HNO3under the assistance of ultrasonication. These SHGOs could be used as fillers for the sulfonated aromatic poly(ether ether ketone) (SPEEK) membrane. The obtained SHGO-incorporated SPEEK membrane has a uniform and dense structure, exhibiting higher performance as proton exchange membranes (PEMs), for instance, higher proton conductivity, lower activation energy for proton conduction, and comparable methanol permeability, as compared to Nafion 112. The sulfonated graphitic structure of the SHGOs is believed to be one of the crucial factors resulting in the higher performance of the SPEEK/SHGO membrane, since it could increase the local density of the −SO3H groups in the membrane and induce a strong interfacial interaction between SHGO and the SPEEK matrix, which improve the proton conductivity and lower the swelling ratio of the membrane, respectively. Additionally, the proton conductivity of the membrane could be further enhanced by the presence of the holes in the graphitic planes of the SHGOs, since it provides an additional channel for transport of the protons. When used, direct methanol fuel cell with the SPEEK/SHGO membrane is found to exhibit much higher performance than that with Nafion 112, suggesting potential use of the SPEEK/SHGO membrane as the PEMs.
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- 2017
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23. Real-time PCR for detection and quantification of C. gloeosporioides s.l. growth in Stylosanthes and Arabidopsis.
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Wan, Miting, Yang, Liyun, Zhang, Shizi, Gao, Jing, Jiang, Lingyan, and Luo, Lijuan
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COLLETOTRICHUM gloeosporioides ,ARABIDOPSIS ,SYMPTOMS ,TROPICAL medicine ,DIAGNOSTIC use of polymerase chain reaction - Abstract
Colletotrichum gloeosporioides sensu lato (s.l.) is one of the most economically and scientifically important fungal pathogens, causing serious diseases in tropical and subtropical region. The detection of C. gloeosporioides s.l. is of importance for disease control. In this study, an ACT-based real-time PCR assay was developed for quantification of C. gloeosporioides s.l., which reliably detected as little as 100 fg genomic DNA, 100 copies of target DNA and 20 conidia. This method could recognize all four tested C. gloeosporioides s.l. isolates, while no amplification was observed in other Colletotrichum species and Botrytis cinerea , indicating the specificity of this assay. Detection and quantification of C. gloeosporioides s.l. was demonstrated in artificially and naturally infected host leaves. First, the real-time PCR analysis was performed using leaf samples collected at different time points post inoculation to monitor the growth of C. gloeosporioides s.l. over time. Secondly, the method was used to compare the resistance in two Stylosanthes cultivars and two Arabidopsis cultivars. Finally, naturally infected and symptomless leaves of Stylosanthes in the fields were tested by the real-time PCR method. Overall, the real-time PCR assay could allow the detection at early symptomless phase of infection; and the results was well correlated with microscopic observation and later disease symptoms. Therefore, our studies have provided a rapid and effective detection method for studying the plant- Colletotrichum interaction as well as disease prediction and control. [Display omitted] • Established a detection method for C. gloeosporioides. • The specificity and sensitivity of the method. • Suitable for the detection in Stylosanthes and Arabidopsis. • Suitable for the natural infected host samples. [ABSTRACT FROM AUTHOR]
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- 2022
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24. Dihydroquercetin suppresses cigarette smoke induced ferroptosis in the pathogenesis of chronic obstructive pulmonary disease by activating Nrf2-mediated pathway.
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Liu, Xiangming, Ma, Yiming, Luo, Lijuan, Zong, Dandan, Li, Herui, Zeng, Zihang, Cui, Yanan, Meng, Weiwei, and Chen, Yan
- Abstract
Background: Dihydroquercetin (DHQ) is a flavonoid with strong anti-inflammatory and antioxidant effects. However, its protective activity against cigarette smoke-induced ferroptosis in the pathogenesis of chronic obstructive pulmonary disease and its underlying mechanisms remain unclear.Purpose: The present study was conducted to investigate the protective role of DHQ in the pathogenesis of COPD in vivo and in vitro.Methods: A cigarette smoke-induced COPD mouse model was established by cigarette smoke (CS) exposure combined with intraperitoneal injection of cigarette smoke extract (CSE). During the modeling process, the mice were intraperitoneally injected with DHQ daily. HBE cells were cultured with CSE with or without pretreatment with DHQ (40, 80 μM) or ML385 (10 μM). Cell viability was assessed by a cell counting kit 8 (CCK-8). The contents of malondialdehyde (MDA) and superoxide dismutase (SOD) were determined by MDA and SOD assay kits, respectively, and reactive oxygen species (ROS) generation was detected by DCFH-DA assays. Protein expression levels of solute carrier family 7 member 11 (SLC7A11), glutathione peroxidase 4 (GPx4) and nuclear factor erythroid 2-related factor 2 (Nrf2) were measured by western blot. Lipid peroxidation was determined by C11-BODIPY staining. Transmission electron microscopy was used to observe the morphological features of the mitochondria.Results: Treatment with DHQ significantly elevated ferroptosis-related protein (SLC7A11 and GPx4) expression in vivo and in vitro. The mRNA levels of SLC7A11 and GPx4 were also increased after DHQ treatment. The excessive MDA and ROS production and depleted SOD activity induced by CSE were reversed by DHQ. DHQ notably reduced the increased lipid peroxidation induced by CSE in HBE cells. In addition, treatment with DHQ attenuated the morphological changes in the mitochondria caused by CSE. Moreover, we also found that DHQ increased the levels of Nrf2 in a concentration-dependent manner in the cigarette smoke-induced COPD mouse model and CSE-treated HBE cells. Additionally, after administering an Nrf2-specific inhibitor, ML385, to HBE cells, the elevated SLC7A11 and GPx4 mRNA and protein levels induced by DHQ were reversed. Moreover, ML385 treatment attenuated the protective effect of DHQ on lipid peroxidation.Conclusion: Our results show that treatment with DHQ significantly reverses the ferroptosis induced by cigarette smoke both in vivo and in vitro via a Nrf2-dependent signaling pathway. [ABSTRACT FROM AUTHOR]- Published
- 2022
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25. Hydrothermal Synthesis of Boron and Nitrogen Codoped Hollow Graphene Microspheres with Enhanced Electrocatalytic Activity for Oxygen Reduction Reaction
- Author
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Jiang, Zhongqing, Zhao, Xinsheng, Tian, Xiaoning, Luo, Lijuan, Fang, Jianghua, Gao, Haoqi, and Jiang, Zhong-Jie
- Abstract
Boron and nitrogen codoped hollow graphene microspheres (NBGHSs), synthesized from a simple template sacrificing method, have been employed as an electrocatalyst for the oxygen reduction reaction (ORR). Because of their specific hollow structure that consists of boron and nitrogen codoped graphene, the NBGHSs can exhibit even high electrocatalytic activity toward ORR than the commercial JM Pt/C 40 wt %. This, along with their higher stability, makes the NBGHSs particularly attractive as the electrocatalyst for the ORR with great potential to replace the commonly used noble-metal-based catalysts.
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- 2015
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26. Quantitative Analysis of Thermal Environment of Adjustable Window Shutters in Summer of Tianjin.
- Author
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Zhang, Haibin, Ma, Jian, Liu, Gang, and Luo, Lijuan
- Subjects
WINDOW blinds ,QUANTITATIVE research ,SUMMER ,CLIMATE change ,THERMAL comfort - Abstract
Abstract: According to the climatic characteristics in Tianjin where lies in the cold climate regions of China, the passage describes a technique called adjustable external blinds which is to enhance the architectural thermal comfort degree. This technique based on a series of experiments of architectures with different orientations in local climatic condition and numerical analysis of thermal environment with finite element method afterwards. This paper shows that alumina shutter has great advantages which can isolate indoor heat greatly in summer of Tianjin, and some optimization measures has also been adopted in order to improve indoor thermal comfort. [Copyright &y& Elsevier]
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- 2011
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27. Study on operating conditions of household vanadium redox flow battery energy storage system
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Zou, Tao, Luo, Lijuan, Liao, Yuwu, Wang, Peiyuan, Zhang, Jun, and Yu, Longhai
- Abstract
•The vanadium redox flow battery energy storage system was built, including the stack, power conversion system, electrolyte storage tank, pipeline system, control system.•By adjusting the system current, the system performance was further studied, including system charge and discharge energy, stack polarization voltage.•The stability of the single battery and the energy consumption of each unit in the system are calculated to provide effective data support for the subsequent improvement of system performance.
- Published
- 2022
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28. Integrated multi-omics analysis provides insights into genome evolution and phosphorus deficiency adaptation in pigeonpea (Cajanus cajan)
- Author
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Liu, Chun, Tai, Yuling, Luo, Jiajia, Wu, Yuanhang, Zhao, Xingkun, Dong, Rongshu, Ding, Xipeng, Zhao, Shancen, Luo, Lijuan, Liu, Pandao, and Liu, Guodao
- Abstract
Pigeonpea (Cajanus cajan) is an important legume food crop and plays a crucial role in a secure food supply in many developing countries. Several previous studies have suggested that pigeonpea has great potential for phosphorus (P) deficiency tolerance, but little is known about the underlying mechanism. In this study, the physiological and molecular responses of pigeonpea roots to phosphate (Pi) starvation were investigated through integrating phenotypic, genomic, transcriptomic, metabolomic, and lipidomic analyses. The results showed that low-Pi treatment increased total root length, root surface area, and root acid phosphatase activity, and promoted the secretion of organic acids (e.g. citric acids, piscidic acids, and protocatechuic acids) and the degradation of phospholipids and other P-containing metabolites in the roots of pigeonpea. Consistent with the morphological, physiological, and biochemical changes, a large number of genes involved in these Pi-starvation responses were significantly upregulated in Pi-deficient pigeonpea roots. Among these Pi-starvation response genes upregulated by low-Pi treatment, four gene families were expanded through recent tandem duplication in the pigeonpea genome, namely phosphate transporter 1(PHT1), phosphoethanolamine/phosphocholine phosphatase(PECP), fasciclin-like arabinogalactan protein(FLA), and glutamate decarboxylase(GAD). These gene families may be associated with Pi uptake from the soil, phospholipid recycling, root morphological remodeling, and regulation of organic acid exudation. Taken together, our results suggest that pigeonpea employs complex Pi-starvation responses to strengthen P acquisition and utilization during low-Pi stress. This study provides new insights into the genome evolution and P deficiency adaptation mechanism of pigeonpea.
- Published
- 2022
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29. A Polypyrrole‐Imprinted Electrochemical Sensor Based on Nano‐SnO2/Multiwalled Carbon Nanotubes Film Modified Carbon Electrode for the Determination of Oleanolic Acid
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Zhang, Zhaohui, Luo, Lijuan, Chen, Hongjun, Zhang, Minglei, Yang, Xiao, Yao, Shouzhuo, Li, Jiaxing, and Peng, Mijun
- Abstract
A sensitive molecularly imprinted electrochemical sensor with specific recognition ability for oleanolic acid was synthesized by modification of multiwalled carbon nanotubes (MWNTs) decorated with tin oxide nanoparticles (nano‐SnO2/MWNTs) and polypyrrole‐imprinted polymer on a carbon electrode. The morphology and electrochemical performance of the imprinted sensor were investigated by using scanning electron microscope (SEM), X‐ray diffraction (XRD), cyclic voltammetry (CV), linear sweep voltammetry (LSV) and amperometric i–tcurve. The results showed that the imprinted sensor displayed excellent selectivity toward oleanolic acid. A linear relationship between the response currents and oleanolic acid concentrations ranging from 5.0×10−8g/L to 2.0×10−5g/L was obtained for the imprinted sensor. The limit of detection (LOD) of the imprinted sensor toward oleanolic acid was calculated as 8.6×10−9g/L at a signal to noise ratio (S/N) of 3. This imprinted sensor was successfully applied to the determination of oleanolic acid in Acitinidia deliciosaroot samples.
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- 2011
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30. Synthesis and application of core‐shell complex‐imprinted polymer for the solid‐phase extraction of melamine from dairy products
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Zhang, Zhaohui, Zhang, Minglei, Luo, Lijuan, Yang, Xiao, Hu, Yufang, Zhang, Huabin, and Yao, Shouzhuo
- Abstract
A core‐shell complex‐imprinted polymer (CIP) was synthesized and evaluated for selective extraction of melamine from dairy products. The CIP synthesis based on surface modification of home‐made silicon microspheres was conducted viaa metal complex as functional monomer, ethylene glycol dimethylacrylate as cross‐link and melamine as template. The resulting CIP was characterized by SEM and Fourier transform infrared spectroscopy. When the amount of silica microspheres was 100 mg and the molar ratio of copper(II) methacrylate to EDGMA was 1:10, well‐dispersed particles were obtained. The complex‐imprinted SPE condition was optimized and the maximum enrichment factor of melamine can be up to 40. The reliability of complex‐imprinted SPE for extraction and enrichment of melamine was investigated and proved by using spiked samples including milk and milk powder with the recoveries ranging from 87 to 92%.
- Published
- 2010
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31. Rapid Synthesis of Single-Crystalline SrSn(OH)6Nanowires and the Performance of SrSnO3Nanorods Used as Anode Materials for Li-Ion Battery
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Hu, Xiaoyan, Tang, Yiwen, Xiao, Ting, Jiang, Jian, Jia, Zhiyong, Li, Dawei, Li, Bihui, and Luo, Lijuan
- Abstract
One-dimensional SrSn(OH)6nanowires were successfully synthesized through a rapid simple methodsonochemical synthesis at room temperature without using any templates or surfactants. The growth mechanism has been proposed, and the reaction conditions were carefully investigated. The experimental results revealed that both the ultrasound irradiation and the presence of Na2CO3in the synthetic process had an impact on the fast formation of the SrSn(OH)6nanowires. Moreover, a subsequent precipitationhydrothermal treatment of SrSn(OH)6nanowireswas conducted in ethanol at 180 °C for 8 h in order to improve their crystallinity and thermal stalibity. SrSnO3nanorods were converted by calcination of the hydrothermally treated SrSn(OH)6nanowires at 700 °C for 3 h in air. In particular, it was the first attempt to test the electrochemical properties of the SrSnO3products as anode in a Li ion battery. The resulting SrSnO3nanorods exhibited a better cyclability over 50 cycles with a reversible lithium storage capacity of 200 mA h g−1than that of SrSnO3nanoparticles.
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- 2010
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32. Investigation on Mechanical Compatibility Matching for Biomedical Titanium Alloys
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Yu, Zhentao, Zhou, L., Luo, Lijuan, Fan, Maohong, and Fu, Yanyan
- Abstract
The effects of alloying elements such as Mo, Sn, Zr, Nb, deforming-rate, solid solution and aging treatment on mechanical property and microstructure of near βtype biomedical Ti alloys based on Ti-Nb-Zr system were studied. The Influential factors and their solution were discussed to keep matching of strength, elastic modulus, plasticity and toughness. Ti-Zr-Mo-Nb and Ti-Zr-Sn-Mo-Nb alloy systems possess excellent biocompatibility, lower elastic modulus, higher strength, finer plasticity and fracture toughness matching by means of solid solution and ageing treatment which are much more suitable for surgical implant and orthopedic materials.
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- 2005
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33. Dietary Fat, but Not Protein or Carbohydrate, Regulates Energy Intake and Causes Adiposity in Mice.
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Hu, Sumei, Wang, Lu, Yang, Dengbao, Li, Li, Togo, Jacques, Wu, Yingga, Liu, Quansheng, Li, Baoguo, Li, Min, Wang, Guanlin, Zhang, Xueying, Niu, Chaoqun, Li, Jianbo, Xu, Yanchao, Couper, Elspeth, Whittington-Davies, Andrew, Mazidi, Mohsen, Luo, Lijuan, Wang, Shengnan, and Douglas, Alex
- Abstract
Summary The impacts of different macronutrients on body weight regulation remain unresolved, with different studies suggesting increased dietary fat, increased carbohydrates (particularly sugars), or reduced protein may all stimulate overconsumption and drive obesity. We exposed C57BL/6 mice to 29 different diets varying from 8.3% to 80% fat, 10% to 80% carbohydrate, 5% to 30% protein, and 5% to 30% sucrose. Only increased dietary fat content was associated with elevated energy intake and adiposity. This response was associated with increased gene expression in the 5-HT receptors, and the dopamine and opioid signaling pathways in the hypothalamus. We replicated the core findings in four other mouse strains (DBA/2, BALB/c, FVB, and C3H). Mice regulate their food consumption primarily to meet an energy rather than a protein target, but this system can be over-ridden by hedonic factors linked to fat, but not sucrose, consumption. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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