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[Display omitted] • The main active ingredients of wolfberry against breast cancer contain quercetin, β-sitosterol, Stigmasterol, etc. • PPI network showed that the core targets of active ingredients of wolfberry against breast cancer were ESR1, MYC, HIF1A, EGFR, VEGFA, CCND1, etc. • Wolfberry may intervene in the process of breast cancer through biological processes such as response to steroid hormones, response to ketones. • The anti-breast cancer process of wolfberry involves several signaling pathways, including cancer pathway, estrogen signaling pathway, etc. This study aims to investigate the therapeutic effects of wolfberry (Lycium barbarum L.) against breast cancer and the potential mechanisms using network pharmacology. The active ingredients and corresponding targets of wolfberry were screened by TCMSP database, and the potential targets of Wolfberry against breast cancer were obtained by intersecting them with the disease targets obtained from GeneCards database and OMIM database；The visualized network of drug-active component-disease-related target was constructed；Protein-protein interaction (PPI) relationships were demonstrated for the wolfberry-breast cancer intersection targets using the String database, and the core target is obtained by analyzing it. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional enrichment analysis were performed on crossover targets via the DAVID online platform and the Bioinformatics online platform. In this study, we screened the main active ingredients of Wolfberry against breast cancer as quercetin, β-sitosterol, Stigmasterol by network pharmacological methods. The PPI network showed that the core targets are ESR1, MYC, HIF1A, EGFR, VEGFA, CCND1etc. GO enrichment analysis showed that the main biological processes of wolfberry against breast cancer include response to steroid hormones, response to ketone, cellular response to chemical stress, epithelial cell proliferation. The results of KEGG enrichment analysis showed that the anti-breast cancer targets of wolfberry were mainly enriched in cancer pathways, estrogen signaling pathway, AGE-RAGE signaling pathway, P53 signaling pathway, HIF-1 signaling pathway. Through a network pharmacology approach, we predicted wolfberry can treat breast cancer mainly by acting on ESR1, MYC, HIF1A and other key targets, regulating cancer pathway, estrogen signaling pathway and so on. [ABSTRACT FROM AUTHOR]

This study aimed to describe the structural features and anti-adipogenesis effect of polysaccharides obtained from Lycium barbarum L. by a bioactivity-oriented purification method. First, crude polysaccharide LICP009 was identified as the main bioactive compound with potential inhibition of intracellular lipid accumulation. Bioactivity guided fractionation was further performed using ion-exchange chromatography and Sephadex G-50 to obtain the purified component LICP009-2-1. This component was mainly composed of arabinose (Ara), galactose (Gal), glucose (Glc), galacturonic acid (GalA), and glucuronic acid (GlcA) in a molar ratio of 40.2:31.5:4.2:5.8:5.7 with a molecular weight of 1.35 × 104 g/mol. Linkage and NMR data indicated that the backbone of LICP009-2-1 was consisted of a repeated unit of →3,6)-β-D-Gal p- (1→ residues, with three branches composed of α-L-Ara f -(1→, α-L-Ara f -(1 → 5)-α-L-Ara f -(1 → 5)-α-L-Ara f -(1 → 3)-β-D-Gal p -(1→, and α-L-Ara f -(1 → 2)-α-L-Ara f -(1→[4)-β-D-Gal p -(1] 3 →. In differentiated 3T3-L1 adipocyte model, LICP009-2-1 exhibited an antiadipogenic effect by decreasing lipid accumulation, as well as downregulating the expression of key transcription factors and proteins in the adipogenesis pathway in a dose-dependent manner. In high-fat-diet-induced obese mice, oral administration of LICP009-2-1 counteracted the progression of obesity and hyperlipidemia. These results indicated that LICP009-2-1 plays anti-adipogenic and anti-obesity roles both in vitro and in vivo , and could be an anti-obesity agent utilized as medicinal, pharmacological and functional food ingredient. • Polysaccharide from Lycium barbarum L. was studied by activity-oriented approach. • LBPs showed anti-adipogenesis activity in differentiated 3T3-L1 adipocytes and high-fat-diet-induced obese mice. • LICP009-2-1 with potential activity was mainly composed of.→3,6)-β-D-Gal p- (1 →. [ABSTRACT FROM AUTHOR]

[Display omitted] • The fermented and unfermented goji berry juices attenuated dextran sodium sulfate induced colitis. • The probiotics-fermented goji berry juice treatment (FGJ, 20 mL/kg/d) prevented histological damage. • FGJ enhanced intestinal integrity and modulated inflammatory cytokines in serum and colon. • The key gut bacteria at the phylums, family and genus level were regulated by FGJ. This study administered the goji juice (20 mL/kg/d) fermented by Lactobacillus plantarum , Lactobacillus reuteri and Streptococcus thermophilus or unfermented juice for male mice for 30 days, then the effects on dextran sodium sulfate (DSS)-induced ulcerative colitis were investigated. The results shown that intaking fermented or unfermented goji juice decreased the levels of pro-inflammatory cytokines and total superoxide dismutase (T-SOD) in serum and colon, while increased the levels of anti-inflammatory cytokines, myeloperoxidase (MPO) and glutathione peroxidase (GSH). Additionally, intaking fermented or unfermented goji juices decreased intestinal permeability, and modulated gut microbiota at the phylums, family and genus level in ulcerative colitis mice. It is worth noting that the probiotics-fermented goji juice showed better anti-inflammatory effects than unfermented goji juice. These results demonstrated that probiotics-fermentation enhanced the anti-ulcerative colitis function of goji berry juice by regulating oxidative stress and inflammation markers and modulating gut microbiota. [ABSTRACT FROM AUTHOR]