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Scopo del presente lavoro è contribuire alla comprensione del significato fisico di alcuni parametri di rugosità in modo da diffonderne l’utilizzo. Si propone inoltre di utilizzarli come metodologia di routine nella definizione dello stato di una superficie.

The synthetic opiate loperamide (LPMD) is an antidiarrheal compound which affects both intestinal motility and the transport of water and electrolytes. We have investigated its effects on ion transport in the stripped ileal mucosa of rabbits, mounted in Ussing chambers in vitro.Addition of LPMD to serosal bathing medium resulted in a dose‐dependent decrement of short‐circuit current (Isc). LPMD (50 μM) significantly increased net Cl absorption (Jc1,) and JRnet(assumed to represent HCO3secretion). LPMD also inhibited the Isc increments evoked either by agents that increase 3‘,5’‐cyclic adenosine monophosphate (cAMP) content (theophylline and prostaglandin E2) or by the Ca2+ionophore A23187; the opiate, however, did not prevent the Isc change induced by 3‘,5’‐cyclic guanosine monophosphate (cGMP)‐related agents such as 8‐Br‐cGMP and Escherichia coliheat‐stable enterotoxin. LPMD did not alter basal or secretagogue‐stimulated tissue cAMP. In contrast, LPMD caused a small increase in cGMP content of stripped ileal mucosa, but not in that of isolated entero‐cytes. The role of Ca2+in LPMD action is suggested by the significantly different effect of the drug on JCtnetin the presence and in the absence of Ca2+in the serosal solution.

The synthetic opiate loperamide (LPMD) is an antidiarrheal compound which affects both intestinal motility and the transport of water and electrolytes. We have investigated its effects on ion transport in the stripped ileal mucosa of rabbits, mounted in Ussing chambers in vitro.Addition of LPMD to serosal bathing medium resulted in a dose-dependent decrement of short-circuit current (Isc). LPMD (50 μM) significantly increased net Cl absorption (Jc1,) and JRnet(assumed to represent HCO3secretion). LPMD also inhibited the Isc increments evoked either by agents that increase 3‘,5’-cyclic adenosine monophosphate (cAMP) content (theophylline and prostaglandin E2) or by the Ca2+ionophore A23187; the opiate, however, did not prevent the Isc change induced by 3‘,5’-cyclic guanosine monophosphate (cGMP)-related agents such as 8-Br-cGMP and Escherichia coliheat-stable enterotoxin. LPMD did not alter basal or secretagogue-stimulated tissue cAMP. In contrast, LPMD caused a small increase in cGMP content of stripped ileal mucosa, but not in that of isolated entero-cytes. The role of Ca2+in LPMD action is suggested by the significantly different effect of the drug on JCtnetin the presence and in the absence of Ca2+in the serosal solution.