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Abstract The effect of an N-alkyl substituent R on the characteristics of ignition of hypergolic ionic liquids (ILs) upon contact with white fuming nitric acid (WFNA) is examined by a modified drop test method that simultaneously combines high-speed shadow imaging with high-speed two-color pyrometry. The number of carbon atoms in the synthesized N-alkylated propargyl-imidazolium dicyanamide with alkyl substituents varied from 1 to 7. The ILs under study can be grouped in two categories depending on the initial location of the flame zone. When R has 1 to 3 carbon atoms, ignition is visualized as a glow inside a bubble (formed after droplet fall) and above the liquid surface. When the number n of carbon atoms in R is 4 to 6, no glow is recorded inside the bubble. In the case of a heptyl substituent, ignition does not occur since no flame is observed during approximately one minute. It is shown that the value of ignition delay can be interpreted as an Arrhenius-type function where the exponent depends linearly on n. An analysis of high-speed video records shows that the evaporation starting time of WFNA measured from the first IL–WFNA contact is also an Arrhenius-type function of n. Furthermore, it is found that the time for a bubble to reach its maximum diameter is independent of n. A possible mechanism of reaction between ILs of this class and WFNA is suggested and discussed. [ABSTRACT FROM AUTHOR]

ICGi021-A and ICGi022-A iPSC lines were obtained by reprogramming PBMCs of two healthy women of the Siberian population using episomal non-integrating vectors expressing Yamanaka factors. iPSC lines expressed pluripotency markers, had a normal karyotype and demonstrated the ability to differentiate into derivatives of the three germ layers. Clinical exome sequencing data of the original biosamples of the donors are available in the NCBI SRA database. The generated cell lines are useful as "healthy" control in biomedical studies. [ABSTRACT FROM AUTHOR]

Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease characterized by death of motor neurons. To date, neither etiology nor pathogenesis of ALS are known, which leads to the absence of an effective treatment strategy. ALS patient-specific induced pluripotent stem cells (iPSCs) represent an excellent tool for the disease study. We obtained iPSCs line from peripheral blood mononuclear cells of the patient with homozygous Asp90Ala mutation in the SOD1 gene using non-integrating episomal vectors. The iPSCs line retained pathological genotype and expressed pluripotency markers. It also displayed a normal karyotype and the ability to differentiate into derivatives of three germ layers. [ABSTRACT FROM AUTHOR]

Studying Parkinson's disease (PD), one of the most common neurodegenerative disorders worldwide, requires different model systems, including patient-specific induced pluripotent stem cell lines. With the help of non-integrating episomal vectors the iPSC lines ICGi015-A and ICGi015-B were generated from blood mononuclear cells of PD patient, carrying three SNPs, associated with PD development. The obtained iPSC lines express pluripotency markers and demonstrate the ability to in vitro differentiate into the three germ layers. These cell lines may be useful for studying molecular mechanisms of PD and for drug screening. [ABSTRACT FROM AUTHOR]

Abstract The induced pluripotent stem cell (iPSC) lines ICGi008-A and ICGi008-B were generated from dermal fibroblasts using episomal vectors expressing pluripotency factors. Dermal fibroblasts were obtained from a 55 year old male Сaucasian familial Alzheimer's disease (AD) patient carrying heterozygous V717I mutation in the APP gene. The generated iPSC lines maintained the original APP genotype, expressed pluripotency markers, exhibited a normal karyotype and retained the ability to differentiate into cell types of the three germ layers. The iPSC lines will be useful for the study of the AD molecular and cellular mechanisms and drug screening. [ABSTRACT FROM AUTHOR]

Abstract Spinal muscular atrophy (SMA) is a neuromuscular disease caused by deletion or mutation in SMN1 gene. SMA human induced pluripotent stem cells (iPSCs) represent a useful and valid model for the study of the disorder, as they provide in vitro the target cells. We generated iPSCs from a SMA type I patient and SMA type II patient by using non-integrating episomal plasmid vectors. The resulting iPSCs are episomal-free, express pluripotency markers, display a normal karyotype, retain the mutation (homozygous deletion of SMN1) and are able to differentiate into the three germ layers. [ABSTRACT FROM AUTHOR]