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Hydrogen-doped alkali-metal fullerides were studied by means of mass-analyzed thermal desorption and positron annihilation. The hydrogen desorption spectra reveal the inclusion of hydrogen in the fullerides as two types of components; one weakly adsorbed and the other strongly bound, probably as hydride ion. The positron lifetime spectra demonstrate that hydrogen-doping to alkali-metal fullerides increases a concentration of higher-order vacancy-type defects, through which more than 50% injected positrons decay with a lifetime of 0.42–0.71 ns.

A novel inorganic synthetic clay material (SC) has been evaluated as the stationary phase in packed-column, supercritical fluid chromatography (SFC). The molecular recognition capability of the SC stationary phase in SFC for polycyclic aromatic hydrocarbons has been evaluated using carbon dioxide and carbon dioxide modified with methanol as the mobile phase. This recognition derives from the layer structure of the SC material which acts as a slit to distinguish non-planar solutes from the molecular-molecular interaction between solute and stationary phase and leads to smaller retention for non-planar solutes. The recognition capability is also dependent on the SFC conditions such as column pressure and column temperature.

Summary: Silicone polymer-coated silica gels modified with octadecyl and octyl groups (S/S-C18, S/S-C8), or “capsule-type silica gels” were developed as packing materials for reversed-phase liquid chromatography. They were obtained by coating the surface of totally porous silica gel with a homogeneous silicone polymer film, and thereafter modifying the coating polymer with octadecyl or octyl groups. Retaining the advantages of silica-based packings, they show strong resistance of alkali-like organic porous polymeric materials.

Summary: A new type of column packing material designed for preparative liquid chromatography, silicone polymer-coated silica gel modified with octadecyl group (S/S-C18), was applied to chromatographic purification of a lipophilic biotechnological product. Triglycerides containing γ-linolenic acid were separated from the curde oil that consisted of triglycerides, diglycerides, free fatty acids, sterols and other polar substances, using a S/S-C18 packed column (150 mm I.D. � 1000 mm). No column deterioration was observed after more than 1500 times of sample introductions.

The nucleotide sequences of the genome RNA encoding the RNA polymerase and the 3′ non-coding region (NCR) of bovine enterovirus (BEV) serotype I Japanese isolate, MZ468, were determined. The genetic distance between the two BEV serotype I strains, MZ468 and VG-5-27, was calculated by pairwise comparison of nucleotide sequences. The synonymous substitution rate was high (1.40 × 10−2/site/year), and of the same order as those of influenza virus HA, HIV-1 gag and env, and enterovirus 70 VP1 genes.