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Abstract Omethoate, an organophosphorous pesticide, causes a variety of health effects, especially the damage of chromosome DNA. The aim of the study was to assess the correlation between polymorphisms of encoding miRNA genes and telomere length in omethoate–exposure workers. 180 workers with more than 8 years omethoate–exposure and 115 healthy controls were recruited in the study. Genotyping for the selected single nucleotide polymorphisms loci were performed using the flight mass spectrometry. Real-time fluorescent quantitative polymerase chain reaction(PCR) method was applied to determine the relative telomere length(RTL) in human peripheral blood leukocytes DNA. After adjusting the covariate of affecting RTL, covariance analysis showed that the female was significantly longer than that of the male in control group(P < 0.046). For the miR-145 rs353291 locus, this study showed that RTL of mutation homozygous AG+GG individuals was longer than that of wild homozygous AA in the exposure group (P = 0.039). In the control group, RTL with wild homozygous TT genotype in miR-30a rs2222722 polymorphism locus was longer than that of the mutation homozygous CC genotype (P = 0.038). After multiple linear regression analysis, the independent variables of entering into the model were omethoate–exposure (b = 0.562, P < 0.001), miR-145 rs353291 (AG+GG) (b = 0.205, P = 0.010). The prolongation of relative telomere length in omethoate exposed workers was associated with AG+GG genotypes in rs353291 polymorphism of encoding miR-145 gene. Graphical abstract fx1 Highlights • The encoding miRNA genes and telomere length under omethoate exposure. • Omethoate-exposure and miR-145 genetic polymorphism affect telomere length. • Prolongation of RTL in workers exposed to omethoate with rs353291 AG+GG genotypes. [ABSTRACT FROM AUTHOR]

Omethoate, an organophosphorous pesticide, can cause a variety of health effects, especially the decrease of cholinesterase activity. The aim of this study is to explore the association of genetic polymorphisms of telomere binding proteins with cholinesterase activity in omethoate-exposed population. Cholinesterase activities in whole blood, red blood cell and plasma were detected using acetylthiocholine and dithio-bis-(nitrobenzoic acid) method; Genetic Genotyping of POT 1 rs1034794, POT 1 rs10250202, TERF 1 rs3863242 and TERT rs2736098 were performed with PCR-RFLP. The cholinesterase activities of whole blood, red blood cells and plasma in exposure group are significantly lower than that of the control group ( P  < 0.001). Multivariate analysis indicates that exposure group ( b  = − 1.016, P  < 0.001), agender ( b  = 0.365, P  < 0.001), drinking ( b  = 0.271, P  = 0.004) and TERF1 rs3863242 ( b  = − 0.368, P  = 0.016) had an impact on cholinesterase activities. The results suggest that individual carrying AG+GG genotypes in TERF 1 gene rs3863242 polymorphism were susceptible to damage in cholinesterase induced by omethoate. [ABSTRACT FROM AUTHOR]

After spraying different concentrations of two brands pesticide omethoate on cole (Brassica campestris L.) leaves, the leaf chlorophyll a fluorescence transients were measure by a Plant Efficiency Annlyzer (PEA), and the toxicological effects and rudimental dynamic courses of on omethoate on the leaf photosystem II (PS II) were investigated by JIP-test. The results showed that after spraying omethoate except at the concentration of 0.50%, the maximal efficiency of photo-chemistry (Fv/Fm) did not have a remarkable change. However , with increasing omethoate concentration, the minimal fluorescence Fo, maximal fluorescence Fm, relative variable fluorescence at the J-step (VJ), and electron transport flux perreactive center in PS II (ETO/RC) increased remarkably, but ψO, the efficiency that a trapped exciton in PS II move an electron into the electron transport chain beyond QA-, decreased remarkably. The test two brands of pesticide omethoate had almost alike elects on the PS II of cole, and the residual effect of the pesticide was the strongest at the third day after spraying and petered out from the ninth to twelfth day. The main targets of omethoate on the PS II of cole could be listed as promoting the reduction from QA to QA- (increasing of VJ) and the electron transmission from QA- to QB (increasing of ETO/RC). [ABSTRACT FROM AUTHOR]