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Abstract A one-step sample processing was developed to determine the levels of perchlorate in human urine, whole blood and breast milk by using liquid chromatography tandem mass spectrometry (LC-MS/MS). Athena C18-WP column was used to separate and analyze perchlorate. Perchlorate and isotope-labeled perchlorate (Cl18O 4 -) internal standards were spiked in the sample matrix through vortex mixing, centrifugation, and filtration. The filtrate was collected and subjected to LC analysis. The developed method was validated for its reproducibility, linearity, trueness, and recovery. Satisfactory recovery of perchlorate ranged from 81% to 117% with intraday relative standard deviations (RSDs) (n = 3) and inter-day RSDs (n = 9) of 5–18% and of 5–16%, respectively. Good linearity (R 2 ≥ 0.99) was observed. Limits of detection and quantification for perchlorate ranged from 0.06 µg/L to 0.3 µg/L and from 0.2 µg/L to 1 µg/L, respectively. Perchlorate concentrations were found in human urine (n = 38) and whole blood (n = 8) samples with the range of 6.5–288.6 µg/L and 0.3–2.8 µg/L, respectively. These results indicate the applicability of our developed method in determining perchlorate level in real samples. Moreover, this method is also highly reliable, sensitive and selective in detecting perchlorate in human urine, whole blood and breast milk samples and may be applicable to other matrixes i.e. saliva, serum, plasma, milk powder and dairy milk. Graphical abstract fx1 Highlights • A one-step sample processing method was developed for perchlorate analysis. • The developed method was rapid, simple, and sensitive. • Perchlorate was detectable in all urine and blood samples. [ABSTRACT FROM AUTHOR]

Triboelectric nanogenerator (TENG) which can work as novel power provider and self-powered sensor has been in rapidly developed recently. However, fabricating large-area TENG through a one-step, continuous, and environmentally friendly method still remains a challenge but highly desired. Herein, large-area sandwiched film based triboelectric nanogenerator (LSF-TENG) was successfully fabricated via coextrusion blow film molding in a one-step way, whose skin (triboelectric layer) and core layer (electrode) are respectively pure polypropylene (PP) and carbon nanotubes compounded with PP (CNTs/PP) phase. Moreover, the as-fabricated LSF-TENG can realize effectively harvest mechanical energy and serves as a self-powered biomimetic whisker (SPBW) for automated guided vehicle (AGV). Furtheromre, thanks to Internet of Things (IoT) technology, the raindrop energy harvesting & precipitation monitoring (REH&PM) system has been successfully developed. This work proposes a facile, continuous and environmentally friendly method for developing low-cost, durable and large-area TENG, opening a new pathway to develop TENG based on the concept of polymer "structuring" processing. [Display omitted] • Large-area sandwiched film based TENG (LSF-TENG) was fabricated via a one-step method. • Triboelectric layer exhibits excellent compatibility with electrode thanks to their shared PP phase. • LSF-TENG can effectively harvests mechanical energy and serves as a self-powered biomimetic whisker. • Raindrop energy harvesting & precipitation monitoring system was constructed. [ABSTRACT FROM AUTHOR]

The article focuses on the study which examines the validation and development of one-step SYBR Green real-time reverse transcription polymerase chain reaction (RT-PCR) method for the rapid detection of Duck Tembusu virus (DTMUV). The study used 96 experimentally infected samples and 168 field samples of ducks. Results show that RT-PCR is cost effective, specific, sensitive, high-throughput, and simple diagnostic tool for the epidemiological surveillance and rapid detection of DTMUV infection.

Objective: The purpose of this study was to determine whether a self-adhesive universal cement, RelyX Unicem (3M ESPE, Seefeld, Germany), can be used successfully to bond orthodontic brackets to enamel. Materials and Methods: Forty human molars were cleaned, mounted, and randomly divided into two groups: 20 orthodontic brackets were bonded to teeth using RelyX Unicem, and 20 brackets were bonded using the Transbond XT (3M Unitex, Monrovia, Calif) adhesive system. The teeth were debonded within 30 minutes after initial bonding using a universal testing machine. After debonding, the enamel surface was examined under 10× magnification to determine the amount of residual adhesive remaining on the tooth. Student's t-test was used to compare the shear bond strength (SBS) of the two groups, and the chi-square test was used to compare the Adhesive Remnant Index (ARI) scores for the two adhesive systems. Results: The mean SBS of the brackets bonded using the RelyX Unicem was 3.7 ± 2.1 MPa and was significantly lower (t = 2.07, P = .048) than the SBS of the brackets bonded with the Transbond system (xˉ = 5.97 ± 4.2 MPa). The comparisons of the ARI scores between the two groups (X2 = 17.4) indicated that bracket failure mode was significantly different (P = .002) with more adhesive remaining on the teeth bonded with Transbond XT. Conclusions: The SBS of the self-adhesive universal cement needs to be increased for it to be successfully used for bonding orthodontic brackets. [ABSTRACT FROM AUTHOR]

Compared to two-step process, one-step spinning process for ITY is more cost-efficient and the product's quality and performance are more stable. Key technologies, similarities and differences of the spinning system and process between high-shrinkage and low-shrinkage POY/FDY blended yarn were introduced in this article. Furthermore, the practical effects of a domestic spinning system is introduced. [ABSTRACT FROM AUTHOR]