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Abstract: The following problem was posed by the second author in ‘Semigroup Forum’, Vol. 1, No. 1, 1970, p. 91: Describe the structure of semigroups S such that for all a, b, c ε S, abc=ab, ac or bc.

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Abstract: It was shown in [3] that every finite group is the maximal subgroup of a semigroupB x of all binary relations on some finite set X. This result is extended here to arbitrary groups.

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Zusammenfassung: Die 11 -HSD knnte ein wichtiger intrazellulrer Regulationsmechanismus sein, welcher bei der Glukokortikoidtherapie Beachtung finden sollte.

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High-frequency electroporation of whole Corynebacterium glutamicum cells without enzymatic pretreatment was achieved. Under optimized conditions concerning growth stage, washing of cells, cell concentration and pulse parameters transformation efficiencies of far more than 107 transformants per μg pWST4B plasmid DNA were reached. Using electroporation, linearised and subsequently religated plasmid as well as chimeric ligase reaction products were directly introduced into C. glutamicum with reasonable efficiencies. Electrotransformation efficiency was reduced about 105-fold for plasmid DNA cycled through E. coli JM83. Restriction deficient mutants of C. glutamicum were isolated which could be efficiently transformed with foreign DNA.