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Grain storage plays a vital part in social stability as well as people’s daily lives. By virtue of its unique storage advantages, wheat has become one of the most important grain reserves in the world. Wheat kernels continue consuming their own energy to maintain lives during the whole process of storage, which will lead to deterioration of their edible qualities as well as may be infected by certain mold provided that the surrounding conditions are suitable. Thus, establishing an efficient and accurate method to assess wheat kernel’s storage status is necessary for the sake of shaping a shelter to human beings as well as animals. For that reason, various wheat aging and wheat moldy detection methods have been summarized in this chapter. Up to now, study of aging and mold detection in wheat kernels can be divided into conventional chemical techniques and newly proposed physical detection methods. This review has analyzed in detail the limitations of conventional technologies, and then probes into some promising physical technologies for better detecting stored status of wheat kernels.