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Copyright of Journal of the Cukurova University Institute of Social Sciences is the property of Cukurova University Institute of Social Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Turkish Culture & Haci Bektas Veli Research Quarterly is the property of Turkish Cultur & Haci Bektas Veli Research Quarterly and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The minimal genetic requirements for microbes to survive within multiorganism communities, including host-pathogen interactions, remain poorly understood. Here, we combined targeted gene mutagenesis with phenotype-guided genetic reassembly to identify a cooperative network of SPI-2 T3SS effector genes that are sufficient for Salmonella Typhimurium (S Tm) to cause disease in a natural host organism. Five SPI-2 effector genes support pathogen survival within the host cell cytoplasm by coordinating bacterial replication with Salmonella -containing vacuole (SCV) division. Unexpectedly, this minimal genetic repertoire does not support S Tm systemic infection of mice. In vivo screening revealed a second effector-gene network, encoded by the spv operon, that expands the life cycle of S Tm from growth in cells to deep-tissue colonization in a murine model of typhoid fever. Comparison between Salmonella infection models suggests how cooperation between effector genes drives tissue tropism in a pathogen group. [Display omitted] • Genome reconstruction reveals essential SPI-2 effector genes of Salmonella • A five-gene network (θ genes) coordinates SCV division and Salmonella proliferation • θ genes and the spv operon cooperate to induce typhoid fever in mice • Host-tissue tropism is determined by diverse effector-gene networks The minimal effector-gene repertoire required for Salmonella intracellular survival and infection of host tissues remains undefined. In this work, Chen et al. performed a systematic interrogation of the SPI-2 effector genome to identify a minimal gene network that drives cellular and tissue tropism in the Salmonella enterica pathogen group. [ABSTRACT FROM AUTHOR]