Search

OBJECTIVE: To investigate the expression of Long non-coding RNA ADIPOQ and its facilitating effects on proliferation and invasion of colorectal cancer by modulating the expression of TP53 via sponging with miR-219c-3p. PATIENTS AND METHODS: qRT-PCR was performed to detect the expressions of ADIPOQ and TP53 in human colorectal cancer tissues and cells. CCK-8 assay was performed to evaluate the Caco-2 cells proliferation and transwell assay was performed to evaluate the Caco-2 cells migration. The relationship between ADIPOQ and miR-219c-3p was detected by statistical analysis. Target prediction and Luciferase activity assay were conducted to investigate the binding site and interaction between ADIPOQ and miR-219c-3p. Further, we cloned the mice TP53 3'-UTR into the Luciferase reporter vector and constructed miR-219c-3p binding mutants to verify the inhibited regulation of miR-219c-3p to the TP53 expression. RESULTS: The results suggested that the expression of ADIPOQ and TP53 was downregulated in human colorectal cancer tissues and Caco- 2 cells. qRT-PCR and CCK-8 assay showed that ADIPOQ expression is correlated with the proliferation of colorectal cancer cells. Transwell assay showed that ADIPOQ regulated the migration ability of colorectal cancer cells. The bioinformatics prediction and Luciferase assay demonstrated that ADIPOQ serves as ceRNA for miR-219c-3p to further regulate the expression of TP53. CONCLUSIONS: For the first time, we found that lncRNA-ADIPOQ was downregulated in human colorectal cancer cells, which could facilitate tumor proliferation, migration and invasion as a ceRNA by sponging with miR-219c-3p. [ABSTRACT FROM AUTHOR]

Iron-based composites were reinforced with TiC particulates by in situ synthesis. The microstructure was characterised by transmission electron microscope. The bright field image, dark field image and diffraction pattern of reinforcing particulates and graphite harmful phase were obtained. Meanwhile, the interface between reinforcing particulates and matrix of TiCp/Fe composite material was also observed by scanning electron microscope and transmission electron microscope. The results showed that the calculated lattice constant of reinforcing TiC particulates was 0·41 nm and the calculated lattice constants of graphite were a = 0·282 nm and c = 0·459 nm. The TiC particulates had quadrilateral morphology, and were chemically wetted in Fe matrix without distinct interface product. There was no transition layer at the interface, avoiding pollution of reinforcing particulates. This study provided some details for understanding the bonding interface of in situ metal matrix composites. [ABSTRACT FROM AUTHOR]