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Seroprevalences were determined by testing sera of 1607 blood donors from North, East, and South Tyrol. In the Tyrols, the continental divide delimitates areas with high seroprevalences of IgG antibodies against Borrelia burgdorferi sensu lato in the North (7.2%) from areas with low seroprevalences in the South (1.5%). To determine Borrelia prevalences in unfed Ixodes ricinus ticks, 755 questing ticks were tested by PCR. Prevalences in nymphal and adult ticks were found to be 19.7% ( n = 132) and 21.5% ( n = 205) in North Tyrol and 23% ( n = 43) and 23.7% ( n = 376) in South Tyrol, respectively. Sequencing of 46 Borrelia -positive ticks yielded 74% Borrelia ( B. ) afzelii , 11% B . garinii , 7% B . lusitaniae , 7% B. burgdorferi sensu stricto, and 2% B. valaisiana infections. Distinct genetic clusters could not be delimitated on either side of the continental divide. This study describes occurrence and geographic dispersion of Borrelia spp. in the Tyrols, discusses possible reasons for significant differences in human seroprevalence, and indicates that prevalence of Borrelia in vector ticks is not a direct predictive factor for the local seroprevalence in humans. [ABSTRACT FROM AUTHOR]

Abstract: Following a brief review of EDM and its use on advanced aerospace alloys including workpiece integrity constraints, data are presented after machining Ti–6Al–4V and Inconel 718. Roughing and finishing (multiple trim cut) strategies were employed on two high specification machines with pulse generators designed to provide minimum workpiece integrity damage. Results include productivity, 3D topographic maps of workpiece surfaces, microstructural and microhardness depth profile data. Average recast thickness was <11μm, several trim passes showing no apparent recast or damage. Similarly, no significant change in workpiece microhardness variation was observed with cracking confined to the recast layer. [Copyright &y& Elsevier]