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Porous defect induced by thermal decomposition is one of the most significant factors in determining the weld quality of ultrasonically welded thermoplastics. MoS2has high thermal stability and a two-dimensional layer structure, which could play a key role in improving the thermal stability of the thermoplastics. In this study, MoS2with 0.5 wt%, 1.0 wt%, 1.5 wt%, and 2.0 wt% loadings are incorporated into carbon fiber/polyamide 66 composite (CF/PA 66), and its effect on microstructure and mechanical properties of the joint is evaluated with the combination of finite element analysis. Results showed the incorporation of MoS2slightly influenced tensile property of the composite while had a considerable effect on joint performance. With 1.5 wt% loading of MoS2, the highest peak load was enhanced to 6.20 kN from 5.05 kN and the difference in peak load became limited. This is mainly because the temperature of the workpiece under the horn rose above the thermal decomposition point of the composite during ultrasonic welding (UW). However, MoS2filler improved the thermal stability of the workpiece and alleviated the thermal decomposition of PA 66 during UW. As a result, the workpiece with MoS2addition can endure longer weld time to increase the weld area of the joint and improve the weld microstructure and porous defect, which results in higher peak load and stability. This work provides a new solution for improving the joint strength of ultrasonically welded thermoplastics.

Electrochemical (EC) sensors have been widely developed for DNA detection, but they are seldom used in a simple, economic, and efficient manner. In this work, for the first time, EC cloth-based DNA sensors (ECDSs) are developed as a new class of EC DNA sensors, without the need for cumbersome chip fabrication and high-cost peripheral facilities. Carbon ink- and solid wax-based screen printing were used to produce ultracheap sensing devices (the cost of one sensor is estimated to be $0.045). Also, a CdTe QDs/MWCNTs nanocomposite (CdTe-MWCNTs) was applied to modify the sensing interface to obtain a stronger EC signal. Specifically, the newly developed double linear hybridization chain reaction (DL-HCR) greatly amplified the EC signal, relative to the conventional linear HCR. Under optimized conditions, target DNA (TD) samples (75-bp DNA fragments prepared via PCR amplification) were determined in a range from 20 fM to 5 nM, with a detection limit of 8.74 fM and relative standard deviations of 2.04% and 4.75% for intra- and inter-assays at 50 pM TD, respectively. Additionally, the ECDSs had an acceptable storage stability and high selectivity. Importantly, the ECDSs, coupled with simple enzyme digestion, could detect genomic DNA from Listeria monocytogenes(L. monocytogenes), and a detection limit of 0.039 ng/μL was obtained. When coupled with enzyme digestion and PCR amplification, the ECDSs could determine L. monocytogenesin milk samples, with detection limits of approximately 1.64 × 104and 11 CFU/mL. These results demonstrate that the method offers a broad prospect for cost-effective, reliable, and highly sensitive gene-sensing applications.

This study addresses the gap in understanding of fabrication solvent influence on Cu-Co catalyst performance for MNZ degradation. By synthesizing recyclable Cu-Co catalysts using the co-precipitation method, the study investigates the impact of ethylene glycol (EG) solvent compared to conventional water (H2O) solvent. The optimized operational parameters resulted in stable and high MNZ degradation efficiency (above 90 % removal) with twice-feeding of 1600 ppm H2O2, pH ∼7.15, and 50 °C. The use of EG solvent enhances catalyst surface area, exposing more active sites with higher Cu+and Cu2+content, which incorporated in CoO6environment with promoted redox property to facilitate H2O2reaction and •OH production. This research contributes valuable insights into catalyst design and optimization, highlighting the potential of EG solvent for industrial application in antibiotic organic pollutant remediation.

Alcohol consumption likely induces gastric carcinogenesis through deregulation of RNA polymerase (Pol) III genes and oxidative damage. Transcription factor IIB-related factor 1 (BRF1) overexpression alleviates RNA Pol III transcription inhibition through breast cancer susceptibility gene 1 (BRCA1). Myeloperoxidase (MPO) involvement in cancer is induced by alcohol-mediated oxidative damage. BRCA1/2 and MPO play key roles in DNA repair. BRCA1 and BRCA2 exert different roles in homologous recombination repair. By using human gastric cancer (GC) biopsies, we investigated the prognostic value of these proteins upon alcohol induction. In total, high expression of BRF1 (P = 0.010) and positive cell infiltration of MPO (P = 0.004) in tumor tissues as well as positive expression of BRCA1 (P < 0.001) in para-tumor tissues were more frequent in GC patients with hazardous or harmful alcohol consumption habits. BRF1 (P = 0.021), BRCA2 (P < 0.001), and MPO (P = 0.039) were independent prognostic factors for disease-free survival. BRCA1 (P = 0.005) and BRCA2 (P < 0.001) also were identified as independent prognostic factors for overall survival. Furthermore, BRCA2 was an independent unfavorable prognostic factor for disease-free survival and overall survival (P < 0.001) in GC patients who underwent platinum-based adjuvant chemotherapy. BRF1, BRCA1/2, and MPO are DNA repair–related biomarkers, induced by alcohol with prognostic value in GC patients.

A comprehensive study of advanced oxidation was conducted over Co-Cu layered double hydroxide (LDH) catalysts, while the Co-Cu interaction mechanism at interfaces as well as oxygen vacancies (Ov) on the production of hydroxyl radicals (•OH) were studied. Cu could promote the oxidation of Co2+by depressing the formation of β-Co(OH)2and replacing Co sites in octahedral environment in α-Co(OH)2. A Co4Cu1LDH catalyst showed the highest sulfadiazine (SDZ) removal efficiency of 93.7%. The reaction mechanism was assumed on the base of kinetics study of SDZ removal. The formation of Co-O-Cu oxo-bridge in MO6surroundings can enhance the generation of ameliorated Ov, which improves the formation of surface hydroxyl for H2O2adsorption by boosting electron-transfer rate under the Co-Cu interaction at interfaces. This study paves a new way to the Fenton-Like catalysts R&D, especially orientating to the remediation of non-biodegradable organic wastes from pharmaceutical industry.

ABSTRACTChloroplast repair and reorganization are crucial for the rehydration of resurrected plants. As one of the most important organelles in plant, photosynthesis takes place in chloroplasts. Meanwhile, light is important to the biosynthesis and activity regulation of chloroplasts. Here, we investigate the recovery of the chloroplasts and photosynthetic system in plant: Selaginella tamariscinaunder dark condition and environmental light (dark-light transition) condition. This study used the S. tamariscinagrown in a culturing room, dehydrated S. tamariscinaand S. tamariscinarehydrated in environmental light and dark conditions for 72 h as experimental material to measure and observed the chlorophyll content, chloroplast ultrastructure, photosynthesis, chlorophyll a fluorescence parameters. Specific leaf area and relative water content recovered in dark-rehydration conditions and were higher than those of light-rehydration, while dark-rehydration did not fully recover the chlorophyll content, net photosynthetic rate, water-use efficiency, nor the Fv/Fm. Dehydration did not destroy the chloroplast envelop, but increased the number of plastoglobules and disturbed the granum structure. As a homeochlorophyllous resurrection plant, reorganization, not the rebuilding of chloroplasts, occurs during the dehydration and rehydration processes in S. tamariscina. Environmental light signals play an important role in the recovery of photosynthetic systems.