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Most truck fleet transportation shows typical repetitive features in vehicle models, routes and cargos. So the internet of vehicles (IoV) theory could be easily introduced into the active control for truck suspensions. We establish a communication network structure in which paired vehicles are basic elements, and the geographic information systems are treated as a detection auxiliary. The new design reduces the overall communication demand for suspension control data. Based on this simplified IoV system, a new super-wheelbase preview control method is proposed. The optimal vehicle distance between paired trucks is calculated by the particle swarm optimisation (PSO). The traditional wheelbase preview algorithm is improved by using two equivalent parameters. The rear truck shows better comprehensive suspension performances than the front one. Finally, we perform a simple objective optimisation in the truck pairs sequence. The convergence results show that with the help of the IoV suspension system, the 6th and after trucks can get the minimised body acceleration in the fleet's first loop.

Our group previously demonstrated that administration of a CD34-negative fraction of human non- hematopoietic umbilical cord blood stem cells (UCBSC) 48 h after ischemic injury could reduce infarct volume by 50% as well as significantly ameliorate neurological deficits. In the present study, we explored possible mechanisms of action using next generation RNA sequencing to analyze the brain transcriptome profiles in rats with ischemic brain injury following UCBSC therapy. Two days after ischemic injury, rats were treated with UCBSC. Five days after administration, total brain mRNA was then extracted for RNAseq analysis using Illumina Hiseq 2000. We found 275 genes that were significantly differentially expressed after ischemic injury compared with control brains. Following UCBSC treatment, 220 of the 275 differentially expressed genes returned to normal levels. Detailed analysis of these altered transcripts revealed that the vast majority were associated with activation of the immune system following cerebral ischemia which were normalized following UCBSC therapy. Major alterations in gene expression profiles after ischemia include blood-brain-barrier breakdown, cytokine production, and immune cell infiltration. These results suggest that UCBSC protect the brain following ischemic injury by down regulating the aberrant activation of innate and adaptive immune responses.

Circadian rhythms regulate cell proliferation and differentiation; however, little is known about their roles in myogenic differentiation. Our synchronized differentiation studies demonstrate that myoblast proliferation and subsequent myotube formation by cell fusion occur in circadian manners. We found that one of the core regulators of circadian rhythms, Cry2, but not Cry1, is critical for the circadian patterns of these two critical steps in myogenic differentiation. This is achieved through the specific interaction between Cry2 and Bclaf1, which stabilizes mRNAs encoding cyclin D1, a G1/S phase transition regulator, and Tmem176b, a transmembrane regulator for myogenic cell fusion. Myoblasts lacking Cry2 display premature cell cycle exit and form short myotubes because of inefficient cell fusion. Consistently, muscle regeneration is impaired in Cry2−/−mice. Bclaf1knockdown recapitulated the phenotypes of Cry2knockdown: early cell cycle exit and inefficient cell fusion. This study uncovers a post-transcriptional regulation of myogenic differentiation by circadian rhythms.

Pluripotent stem (PS)-cell-derived cell types hold promise for treating degenerative diseases. However, PS cell differentiation is intrinsically heterogeneous; therefore, clinical translation requires the development of practical methods for isolating progenitors from unwanted and potentially teratogenic cells. Muscle-regenerating progenitors can be derived through transient PAX7 expression. To better understand the biology, and to discover potential markers for these cells, here we investigate PAX7 genomic targets and transcriptional changes in human cells undergoing PAX7-mediated myogenic commitment. We identify CD54, integrin α9β1, and Syndecan2 (SDC2) as surface markers on PAX7-induced myogenic progenitors. We show that these markers allow for the isolation of myogenic progenitors using both fluorescent- and CGMP-compatible magnetic-based sorting technologies and that CD54+α9β1+SDC2+ cells contribute to long-term muscle regeneration in vivo. These findings represent a critical step toward enabling the translation of PS-cell-based therapies for muscle diseases.

ABSTRACTTo apply the Ziyuan-3 (ZY-3) for monitoring of sea state, this letter proposes an ocean integrated wave parameter retrieval model based on analysis of the spatial characteristics of ZY-3 satellite optical imagery. Using pseudo-top-hat operator, it retrieves significant wave height, peak wavelength and direction from ZY-3 images based on their spatial characteristics and the principles of optical shadowing of rough ocean surfaces. The experiment demonstrates the feasibility of this wave model capturing integrated wave parameters from ZY-3 images, with a maximum significant wave height error of 0.27 m and a maximum peak direction error of under the slight-moderate sea state in range 0.5–2.5 m swell waves, and the merit that the retrieval of integrated wave parameters does not depend on other measured data or the modulation transfer functions.