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Recently, Advanced Platelet Rich Fibrin (A-PRF) has been considered an alternative to fetal bovine serum (FBS) as a culture medium supplement for cells. A-PRF can release the growth factors (GF) that are required for fibroblast proliferation. However, the ability of A-PRF to support proliferation in human Dental Pulp Stem Cells (hDPSCs) has not been reported yet. We analyzed hDPSC proliferation from cultures in three different concentrations of A-PRF cultured medium (10%, 20%, and 25% A-PRF) using flow cytometry and an MTT assay compared with a control group. Compared with 10% and 20% A-PRF, the proliferation of hDPSCs in 25% A-PRF cultured medium was significantly different on the first day (P<.05). The proliferation rate of hDPSCs between days 1 and 3 and days 1 and 5 was significantly different (P<.05) in the different media. There were no significant differences in the proliferation rate between days 3 and 5 (P>.05). The highest rate of proliferation was observed with 25% A-PRF on day 1 compared with 10% and 20% A-PRF. [ABSTRACT FROM AUTHOR]