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In this study, Listeria monocytogenes and Escherichia coli were selected as reference bacteria, and the inhibitory effects and mechanisms of cell-free supernatants (CFS) of Limosilactobacillus fermentum 733 on its free-living bacteria and biofilm were investigated in depth. The findings demonstrated that L. fermentum 733 CFS displayed notable suppressive effects on L. monocytogenes and E. coli , which were caused by the loss of the cell membranes' structural integrity. This disturbance resulted in the release of intracellular adenosine triphosphate (ATP), alkaline phosphatase (ALP), and reactive oxygen species (ROS). Scanning electron microscopy and laser scanning confocal microscopy showed that L. fermentum 733 CFS disrupted L. monocytogenes and E. coli biofilm structure. L. fermentum 733 CFS inhibited partial population sensing and biofilm-associated gene expression of L. monocytogenes and E. coli, shown by fluorescence quantitative PCR (qPCR). Metabolomics analysis showed that L. fermentum 733 CFS strongly inhibited L. monocytogenes and E. coli by the disruption of both cellular structure and intracellular. These results offer novel perspectives and a theoretical foundation for the prevention of lactic acid bacteria (LAB) infections caused by L. monocytogenes and E. coli. • Limosilactobacillus fermentum 733 CFS disrupted the structural cell integrity of the pathogens. • Limosilactobacillus fermentum 733 CFS affects the biofilm structure of pathogenic bacteria. • Limosilactobacillus fermentum 733 CFS inhibits the expression of genes in pathogenic bacteria. • Limosilactobacillus fermentum 733 CFS disrupts the metabolic pathways of pathogenic bacteria. [ABSTRACT FROM AUTHOR]

Copyright of China Journal of Oral & Maxillofacial Surgery is the property of Shanghai Jiao Tong University, College of Stomatology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

A combined method of catalytic infrared (CIR) and intense pulsed light (PL) was investigated to settle a dispute of bacteriostasis on green Sichuan pepper (GSP), and the synergistic principle was elucidated from several aspects. The synergistic bacteriostasis was confirmed first on GSP by the plate counting method. On bacterial cells, leakage of cytoplasmic contents, intracellular protein and DNA breakages were investigated, and the difference between CIR and PL indicated the possibility of synergism. On a single bacterium, the expected synergy was not achieved on the plate but was obtained on GSP, and the bacterial cells treated by the combined method were mainly broken under SEM, indicating that the alliance technology could increase the damage of PL to bacterial cells on the rough surface of GSP. On multiple bacteria, synergy was obtained similarly, and the bacterial community on GSP was investigated with high-throughput sequencing. The community composition on GSP could be changed by the treatments. In conclusion, synergistic bacteriostasis on GSP was achieved by a combined method of CIR and PL, originating from a complementary effect on the microbiome and a promotion of bacteriostatic capacity on rough surfaces. These findings are expected to solve the bacteriostatic difficulty of spices with rough surfaces and have important implications for the development of effective bacteriostatic strategies in food preservation. • A combined CIR-PL conception was developed for bacteriostasis on rough surface. • A synergistic principle came from the promotion on rough surface by CIR pretreatment. • Another synergistic principle was interpreted based on microbiome complementarily. [ABSTRACT FROM AUTHOR]

This paper aimed to study the effect of metronidazole sustained-release film combined with minocycline hydrochloride on treating periodontitis. 138 patients with chronic periodontitis were collected and randomly divided into control group and observation group (each of 69 cases). Patients in control group were treated by using minocycline hydrochloride, while those in observation group were treated using metronidazole sustained-release film with minocycline hydrochloride, and each group with 4 weeks of treatment. Then we observed the periodontal mend matters, therapeutic effect, adverse reaction and relapse situation of patients in two groups. Results showed that compared with the prior treatment, the gingival index, dental plaque index, odontoseisis index and pocket depth of patients in two groups was significantly reduced. Moreover, the reduction range in observation group was significantly larger and the curative effect was superior to that in control group. Therefore, metronidazole sustained-release film combined minocycline hydrochloride can evidently improve patients' periodontal status, enhance drug therapeutic effect. It has less adverse reaction and low relapse rate, thus is worthy of clinical promotion. [ABSTRACT FROM AUTHOR]

To explore the regulatory mechanisms of parasitism of Sclerodermus sichuanensis on Tenebrio molitor, the methods of natural parasitism and venom injection were adopted to investigate the effects of the venom from S. sichuanensis on the pupa of T. molitor in the parasitic process. Under venom injection, the paralytic degree of the pupa had a positive correlation with the concentration of injected venom, and the number of recovered pupa had a negative correlation with the injected venom concentration. The T. molitor pupa was in slight and reversible paralysis when injected with 0. 01 VRE (venom reservoir equivalent) of venom, and in non-reversible and complete paralysis when 0. 2 VRE was injected. The pupa died massively and appeared a wide range of melanization when injected with soil bacterial suspension alone, but the melanization delayed and the mortality declined significantly when the mixed liquor of bacterium and venom was injected. The bacterio stasis of the venom on Staphylococcus aureus was significantly stronger than that on Escherichia coli. Within a definite range of temperature, the paralytic activity decreased significantly with increasing temperature, the bacteriostasis on S. aureus increased significantly, while that on E. coli was opposite. This study showed that the venom from S. sichuanensis had the effects of paralysis, bacterio-stasis, inhibiting exuviations, and delaying melanization. [ABSTRACT FROM AUTHOR]

The article focuses on a study on the antivirus and antibactericidal effects of flavones extracted from phoenix tree flower. It states that in the study, the nutrient agar medium was used to study the antibacterial of pathogenic bacteria. It mentions that minimal inhibitory concentration of flavones against tested bacteria was measured by cell culture. It reports the best antibacterial effect of the bacteriostasis in flavones against Micrococcus tetragenus, Escherichia coli and sarcomas.

A laboratory test was conducted to study the control effect and bacteriostasis of antagonistic bacterium Burkholderia cepacia Lu10-1 isolated from mulberry on silkworm septicemia, aimed to develop a new microbial pesticide to control silkworm diseases. The supernatant of Lu10-1 zymotic fluid achieved 41.2% control efficiency and 24.0% prophylactic effect on silkworm septicemia. The antibacterial crude extract of Lu10-1 had stronger antagonistic activity against Bacillus bombyseptieus. The diameter of inhibition zone reached 18.20 mm, and the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of the antibacterial crude extract were 1.56 and 3.13 mg· mL-1, respectively. After treated with the antibacterial crude extract, B. bombyseptieus never appeared logarithmic growth phase, its cell membrane permeability changed, intracellular protein leaked out, intracellular macromolecular protein degraded, and at last, the thalli cracked, inner substances out-flowed, cavity formed, and cell ablated. It was considered that the antagonistic substances of Lu10-1 strain could be used for controlling silkworm septicemia, with preferable development foreground. [ABSTRACT FROM AUTHOR]

Abstract: The raspberry healthy draft beer was obtained by adding the extracting of raspberry into the raw fermentation materials of beer, in order to prolong the shelf life, the different concentrations nisin were added to the the raspberry healthy draft beer, the result shown that nisin could have obvious influence on the shelf life, the shelf life prolonged with nisin concentrations adding, the shelf life raspberry healthy draft beer was 44d after adding 0.02 nisin mg/mL, the shelf life prolonged 36d than blank. [Copyright &y& Elsevier]

Summary: Tissue banks culture tissue specimens to confirm the absence of viable micro-organisms after decontamination with antibiotics. It is possible that antibiotic residues attached to decontaminated tissue are introduced into enrichment culture media. These could have an inhibitory effect on the culture results and generate false-negative results. Our aim was to detect bacteriostasis in Wilkins–Chalgren broth inoculated with bone and tendon remnants. These remnants had been soaked in a solution containing gentamicin as part of the tissue-processing procedure. We used the United States Pharmacopeia method for bacteriostasis testing with gentamicin-susceptible Pseudomonas aeruginosa American Type Culture Collection (ATCC) 15442, Staphylococcus aureus ATCC 6538, Bacillus subtilis ATCC 6633 as test strains, and gentamicin-resistant Candida albicans ATCC 90029 as control. The residual gentamicin concentration in the broths was determined and gentamicin-soaked tissue was placed on Mueller–Hinton agar inoculated with a staphylococcal suspension. Bacteriostasis was present in 53–75% of the reference test strains. Tendon remnants had a significantly higher rate of bacteriostasis (85%) than bone remnants (28%). Broths inoculated with tendon remnants had the highest residual gentamicin concentrations. [Copyright &y& Elsevier]

PURPOSE: To evaluate the pharmacologic effect of garlic on oral bacteriostasis and root detoxification. METHODS: Eight representative oral anaerobes (58 strains) including Streptococcus (9), Peptococcus (4), Lactobacillus (6), Actinomyces (3), Veillonella (10), Porphyromonas (12), Prevatella (3), Fusobacterium (11) to garlic juice were systematically studied by classical tube susceptibility test. The periodontally involved roots (12) were selected and the slices (6mmx3mmx1mm) were prepared on the exposed root surface. Six slices were randomly treated by garlic juice for 5 minutes; the other six slices were treated by normal saline with same method as control. The attachment of cultured L-fibroblasts to the treated root surface was observed under phase contrast microscope and electron scanning microscope. The attachment cells were statistically analyzed using SPSS10.0 software package for Student's t test. RESULTS: The minimal inhibitory concentration of garlic juice on most oral anaerobes (87.93%) was 1:64-1:512. The attachment L-fibroblasts to the garlic treated group increased significantly vs. the control group (P<0.05). CONCLUSION: The study shows that garlic juice could effectively inhibit oral anaerobes and reestablish the root biological adaptation. [ABSTRACT FROM AUTHOR]

Various herbal species were screened for their antimicrobial activities by employing preliminary (qualitative) antimicrobial tests. Methanolic extraction procedure was followed for extracting the active substances from herbs. Antimicrobial efficacy was assayed by AATCC (agar diffusion and parallel streak) method and Hohenstein modified challenge test. The neem leaves (Azadirachta indica), prickly chaff flower (Achyranthus aspera), tulsi leaves (Ocimum basilicum) and pomegranate rind (Punica granatum) were found to exhibit antimicrobial activity against the strains of Staphylococcus aureus and E. coil Neem ranked first followed by pomegranate and prickly chaff flower. Despite the negative results of tulsi in the qualitative tests, it showed 73% bacterial reduction in the quantitative challenge test. The treated fabric samples exhibited resistance to degradability as tested by digging soil test. [ABSTRACT FROM AUTHOR]

The study reports a facile synthesis of Ag-g-C 3 N 4 /AC composites with highly photocatalytic activity and good adsorption capacity by an impregnation-thermal polycondensation process. The resulting Ag-g-C 3 N 4 /AC composites were used for treatment of different pollutants in water including Rhodamine B (RhB), Cr(VI) ions and pathogens. When the precursor mass ratio of urea and AC was 2:2, the resultant Ag-g-C 3 N 4 /AC catalyst showed the best photocatalytic performances. In particular, the composite photocatalysts exhibited highly bactericidal efficiency, which could reach 99.9% after 120 min of irradiation. The higher photocatalytic performances of Ag-g-C 3 N 4 /AC composites were attributed to the surface plasmon resonance (SPR) effect of Ag nanoparticles (NPs) together with the interfacial synergistic effects. Meanwhile, AC not only exhibited excellent adsorption capacity, but also decreased aggregation of g-C 3 N 4 , which could enhance the separation efficiency of photogenerated charge carriers. It was uncovered through Electron Spin Resonance (ESR) and radical scavenger experiments that •OH radicals, •O 2 − and h+ participate in the RhB degradation under the visible light. This work shows that the high-efficient and stable Ag-g-C 3 N 4 /AC composites have a great potential in the treatment of organic pollutants, toxic heavy metals and pathogenic bacteria by adsorption/photocatalysis for practical water treatment. The Ag-g-C 3 N 4 /AC photocatalysts exhibit excellent activities in the photocatalytic degradation of RhB, Cr(VI) reduction and bacteriostasis of E.Coli. under visible light irradiation. [Display omitted] • Novel Ag-g-C 3 N 4 /AC photocatalysts were successfully fabricated. • The catalysts exhibited excellent properties in water treatment. • The Ag-g-C 3 N 4 /AC(2:2) afforded the highest photocatalytic activities. • •OH radicals, •O 2 − and h+ participate in the photocatalytic degradation of RhB. • The recycling experiments showed the stability and reusability of the catalysts. [ABSTRACT FROM AUTHOR]

Copyright of China Textile Leader is the property of China Textile Information Center and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)