1. DNA damage induced during mitosis undergoes DNA repair synthesis
- Author
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Godinez, Veronica Gomez, Kabbara, Sami, Sherman, Adria, Wu, Tao, Cohen, Shirli, Kong, Xiangduo, Maravillas-Montero, Jose Luis, Shi, Zhixia, Preece, Daryl, Yokomori, Kyoko, and Berns, Michael W
- Subjects
Biological Sciences ,Environmental Biotechnology ,Environmental Sciences ,Cancer ,Genetics ,1.1 Normal biological development and functioning ,2.1 Biological and endogenous factors ,Generic health relevance ,Animals ,Cell Line ,DNA ,DNA Breaks ,DNA Repair ,G1 Phase ,Humans ,Infrared Rays ,Lasers ,Mitosis ,Potoroidae ,ATM protein ,BRCA1 protein ,discoidin domain receptor ,DNA ligase ,DNA ligase IV ,gamma H2AX ,histone H2AX ,nibrin ,Rad51 protein ,tumor suppressor p53 binding protein 1 ,ubiquitin ,unclassified drug ,anaphase ,animal cell ,Article ,cell cycle G1 phase ,cell damage ,controlled study ,DNA damage ,DNA repair ,DNA synthesis ,double stranded DNA break ,homologous recombination ,human ,human cell ,metaphase ,mitosis ,nonhomologous end joining repair ,nonhuman ,potoroo ,Potorous tridactylus ,protein function ,protein localization ,regulatory mechanism ,adverse device effect ,adverse event ,animal ,biosynthesis ,cell line ,DNA strand breakage ,genetics ,infrared radiation ,laser ,radiation response ,rat kangaroo ,General Science & Technology - Abstract
Understanding the mitotic DNA damage response (DDR) is critical to our comprehension of cancer, premature aging and developmental disorders which are marked by DNA repair deficiencies. In this study we use a micro-focused laser to induce DNA damage in selected mitotic chromosomes to study the subsequent repair response. Our findings demonstrate that (1) mitotic cells are capable of DNA repair as evidenced by DNA synthesis at damage sites, (2) Repair is attenuated when DNA-PKcs and ATM are simultaneously compromised, (3) Laser damage may permit the observation of previously undetected DDR proteins when damage is elicited by other methods in mitosis, and (4) Twenty five percent of mitotic DNA-damaged cells undergo a subsequent mitosis. Together these findings suggest that mitotic DDR is more complex than previously thought and may involve factors from multiple repair pathways that are better understood in interphase.
- Published
- 2020