1. Bacterial biogeography of adult airways in atopic asthma
- Author
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Durack, Juliana, Huang, Yvonne J, Nariya, Snehal, Christian, Laura S, Ansel, K Mark, Beigelman, Avraham, Castro, Mario, Dyer, Anne-Marie, Israel, Elliot, Kraft, Monica, Martin, Richard J, Mauger, David T, Rosenberg, Sharon R, King, Tonya S, White, Steven R, Denlinger, Loren C, Holguin, Fernando, Lazarus, Stephen C, Lugogo, Njira, Peters, Stephen P, Smith, Lewis J, Wechsler, Michael E, Lynch, Susan V, Boushey, Homer A, and for the National Heart, Lung and Blood Institute’s “AsthmaNet”
- Subjects
Lung ,Asthma ,Clinical Research ,2.1 Biological and endogenous factors ,Aetiology ,Respiratory ,Adult ,Bronchi ,Corynebacterium ,Eosinophils ,Female ,Humans ,Inflammation ,Male ,Microbiota ,Middle Aged ,Moraxella ,Mouth Mucosa ,Nose ,RNA ,Ribosomal ,16S ,Sputum ,Adult asthma ,Atopy ,Upper airways ,Lower airways ,Bronchial microbiota ,Nasal microbiota ,Induced sputum microbiota ,Oral microbiota ,Eosinophilic inflammation ,National Heart ,Lung and Blood Institute’s “AsthmaNet” ,Ecology ,Microbiology ,Medical Microbiology - Abstract
BackgroundPerturbations to the composition and function of bronchial bacterial communities appear to contribute to the pathophysiology of asthma. Unraveling the nature and mechanisms of these complex associations will require large longitudinal studies, for which bronchoscopy is poorly suited. Studies of samples obtained by sputum induction and nasopharyngeal brushing or lavage have also reported asthma-associated microbiota characteristics. It remains unknown, however, whether the microbiota detected in these less-invasive sample types reflect the composition of bronchial microbiota in asthma.ResultsBacterial microbiota in paired protected bronchial brushings (BB; n = 45), induced sputum (IS; n = 45), oral wash (OW; n = 45), and nasal brushings (NB; n = 27) from adults with mild atopic asthma (AA), atopy without asthma (ANA), and healthy controls (HC) were profiled using 16S rRNA gene sequencing. Though microbiota composition varied with sample type (p
- Published
- 2018