1. Structure of promoter-bound TFIID and model of human pre-initiation complex assembly
- Author
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Louder, Robert K, He, Yuan, López-Blanco, José Ramón, Fang, Jie, Chacón, Pablo, and Nogales, Eva
- Subjects
Biochemistry and Cell Biology ,Biological Sciences ,Genetics ,1.1 Normal biological development and functioning ,Cryoelectron Microscopy ,DNA ,Humans ,Models ,Molecular ,Promoter Regions ,Genetic ,Protein Binding ,Substrate Specificity ,TATA Box ,TATA-Binding Protein Associated Factors ,TATA-Box Binding Protein ,Transcription Factor TFIIA ,Transcription Factor TFIID ,Transcription Initiation ,Genetic ,General Science & Technology - Abstract
The general transcription factor IID (TFIID) plays a central role in the initiation of RNA polymerase II (Pol II)-dependent transcription by nucleating pre-initiation complex (PIC) assembly at the core promoter. TFIID comprises the TATA-binding protein (TBP) and 13 TBP-associated factors (TAF1-13), which specifically interact with a variety of core promoter DNA sequences. Here we present the structure of human TFIID in complex with TFIIA and core promoter DNA, determined by single-particle cryo-electron microscopy at sub-nanometre resolution. All core promoter elements are contacted by subunits of TFIID, with TAF1 and TAF2 mediating major interactions with the downstream promoter. TFIIA bridges the TBP-TATA complex with lobe B of TFIID. We also present the cryo-electron microscopy reconstruction of a fully assembled human TAF-less PIC. Superposition of common elements between the two structures provides novel insights into the general role of TFIID in promoter recognition, PIC assembly, and transcription initiation.
- Published
- 2016