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Due to the large amount of pollution at coal spoil heaps, soil reclamation is slow and requires development and use of microbial preparations, selected based on assessments of the biochemical parameters of soil and the extent of its contamination. The material for this study was mine soils sampled in three different zones (Ka, Kb, Kc) of the Korchakol coal spoil heap. The studied sanitary and chemical indicators did not exceed the approximate permissible concentrations (APC) / maximum allowable concentrations (MAC), except for oil products (in the Ka, Kb, Kc zones, the excess averaged 2.2, 1.4, 1.2 times, respectively). The study found a direct correlation between the zinc content and the enzymatic activity of polyphenol oxidase, the level of Ni and peroxidase, as well as an inverse correlation between the As and Cu concentrations with invertase and nitrite reductase, respectively. The index of bacteria of the Escherichia coli group (coliform bacteria) in different zones exceeded the standard values by 61 and 171 times. Even though the sanitary indicators studied do not exceed permissible levels, they can slow down growth and development of plants. Thus, successful reclamation of mine soils requires introduction of biological preparations based on microorganisms that biotransform heavy metals and rhizobacteria.