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Introduction:Motivation is an important tool to motivate nurses to provide quality care. The purpose of this study was to investigate the decreasing factors of motivation affecting the performance of nurses in the teaching hospitals of Shiraz University of Medical Sciences. Methods: This descriptive-analytic study was conducted in Shiraz in 2019. The research population consisted of all nurses of the teaching hospitals of Shiraz University of Medical Sciences, among whom 340 from 10 hospitals were selected as sample size. Data were collected through a questionnaire and analyzed by t-test, ANOVA, and Pearson correlation coefficient with SPSS23 software. Results: The total mean of materialistic and non-materialistic factors decreasing motivation were 3.14 ± 1.24 and 3.29 ± 1.31, respectively. Among the materialistic factors, the "personal economic problems" factor with the mean of 3.38 ± 1.14 and among non-materialistic factors, the "instability in decision making by the top managers" with the mean of 4/31 ± 1/13 had the highest score. There was a significant relationship between materialistic factors and gender, marital status, shift record, the number of shift work per month, and the number of patients under observation in each shift work. Also, there was a significant relationship between non-materialistic factors and age, work experience, and education level (p˂0.05). Conclusion: According to the findings, organizing training courses for top managers in different managerial levels in the field of decreasing factors of motivation, providing a suitable working environment coupled with the desired interaction, and solving the economic problems of nurses is suggested.