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This paper presents a novel device which provides the opportunity to perform high-throughput biochemical assays on different individual cells. In particular, the proposed device is suited to screen the rare cells in biological samples for early stage cancer diagnosis and explore their biochemical functionality. In the process, single cells are precisely positioned and captured in activated micropores. To show the performance of the proposed device, cultured yeast cells and human epithelial circulating tumor cells are successfully captured.

This paper presents the modeling of the electrical properties of bioactivated nanopores, customized nanopore devices with a biological macromolecule attached in the pore as the probe. These devices are capable of detecting and analyzing interactions between the attached biomolecule and the molecules in the analyte at a single molecule level. I. INTRODUCTION lthough nanopore-based sensors have long been explored for single nucleic acid molecule sequencing (1), the researchers still cannot claim success. Nonetheless, nanopores offer one of the most promising approaches for structural analysis of single molecules with a diameter of a few nanometers. Nanometer-scale pores provide an electronic way of probing the structure of a single biomolecule. Bioactivated nanopores have many significant potential applications for bio-analysis in a label-free platform at the single molecule level. Any chemical binding or minor structural changes in the biomolecule can be detected by monitoring the ionic current passing through the nanopore. As a result, these devices can be used to detect the interactions between the biomolecule attached in the pore and the molecules in the analyte at the single molecule level.