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Start Over Author "Chunlu Zhang" Journal 2009 3rd international conference on bioinformatics and biomedical engineering
1 results on '"Chunlu Zhang"'

1. Effect of N-Terminal and C-Terminal Deletion of Cry1Ie of Bacillus thuringiensis on Its Expression and Purification

Author

Chunlu Zhang, Fuping Song, Shuyuan Guo, Jie Zhang, and Huicong Shen

Subjects
chemistry.chemical_classification, biology, fungi, Mutant, food and beverages, Plutella, biology.organism_classification, medicine.disease_cause, Inclusion bodies, Microbiology, Amino acid, Biochemistry, chemistry, Bacillus thuringiensis, medicine, bacteria, Gene, Escherichia coli, Ostrinia furnacalis
Abstract

Crystal proteins are produced by the gram-positive bacterium Bacillus thuringiensis (Bt) during sporulation. The cry1le genes were silent in Bt strains, but can be over expressed in Escherichia coli BL21 (DE3) in inclusion bodies, and the expressed product was toxic to Plutella xylostella, Ostrinia furnacalis, and soybean pod borer (Leguminivora glycinivorella). In order to know the N-terminal and C-terminal amino acid on the structure formation of Cry1le protein, four deletion mutants were constructed and the expression and purification of these mutants were studied.

Published
2009
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