1. A microfluidic device capable of continuous quantification of single-cell specific membrane capacitance and cytoplasm conductivity
- Author
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Deyong Chen, Yana Luo, Jian Chen, Rong Long, Min-Hsien Wu, Junbo Wang, Song-Bin Huang, Yang Zhao, Tzu-Keng Chiu, Hao Li, and Bin Deng
- Subjects
Membrane potential ,Membrane ,Materials science ,Cytoplasm ,Analytical chemistry ,Equivalent circuit ,Conductance ,Conductivity ,Capacitance ,Electrical impedance - Abstract
This paper presents a microfluidic system enabling continuous characterization of specific membrane capacitance (Cspecific membrane) and cytoplasm conductivity (σcytoplasm) of single cells by aspirating cells continuously through a constriction channel with cell elongations and impedance profiles at two frequencies (1 kHz and 100 kHz) measured simultaneously. 1 kHz impedance data were used to evaluate cellular sealing properties and 100 kHz impedance data were used to quantify membrane capacitance and cytoplasm conductance, which were than translated to Cspecific membrane and σcytoplasm based on the proposed equivalent circuit model. Four cell lines were used to evaluate this technique, producing Cspecific membrane and σcytoplasm of 3.81±0.90 μF/cm2 and 0.49±0.07 S/m (CRL-5803, n=489), 4.28±1.05 μF/cm2 and 0.45±0.08 S/m (CCL-185, n=487), 3.67±1.00 μF/cm2 and 0.47±0.09 S/m (CRL-1932, n=302), as well as 4.53±1.51 μF/cm2 and 0.55±0.14 S/m (CRL-1999, n=216), respectively.
- Published
- 2013