1. Delivery system for AZT-triphosphate analogues based on SiO2 nanoparticles
- Author
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N. A. Mazurkova, Vladimir N. Silnikov, Asya S. Levina, Nikolai Li-Zhulanov, Natalia V. Shatskaya, Svetlana V. Vasilyeva, and Kasivelu Govindaraju
- Subjects
Materials science ,biology ,Cycloaddition ,chemistry.chemical_compound ,chemistry ,Biochemistry ,Sio2 nanoparticles ,Nucleoside triphosphate ,biology.protein ,Click chemistry ,Delivery system ,Nucleoside ,Polymerase ,DNA - Abstract
SiO2 nanoparticles as platform for delivery of 3′-triazole analogues of AZT-triphosphate into cells were proposed. The results confirmed the successfulness of the Huisgen's 1,3-dipolar cycloaddition reaction for the preparation of the SiO2∼dNTP nanocomposites. It was shown that polymerase can recognize nucleoside triphosphates in the proposed nanobiocomposites and incorporate them into the growing DNA chain. The proposed SiO2∼dNTP nanocomposites were demonstrated to penetrate into eukaryotic cells. The tentative results indicate that these nanocomposites at low concentrations can inhibit the reproduction of POX and Herpes viruses. This makes it possible to use the nanobiocomposites bearing nucleoside triphosphate analogues as promising therapeutic drugs.
- Published
- 2015
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