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Animal feed with meat and bone meal (MBM) has been the source of bovine spongiform encephalopathy (BSE) in cattle and other livestock animals. Many countries have banned the use MBM as animal feed ingredient. Spectral imaging techniques have shown potentials for rapidly assessing authentication of various food and feed ingredients. The aim of this study was to develop rapid and accurate spectral imaging methods for assessing MBM in animal feed. A preliminary investigation of hyperspectral fluorescence imaging techniques for assessing pork and poultry MBM characteristics in animal feeds is presented in this paper.