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This work presents a biodegradable flexible pressure sensor using a piezoelectric layer sandwiched between Mg electrodes. The active layer of the sensor is a piezoelectric composite of β-glycine amino acid and natural chitosan polymer, fabricated by self-assembly of glycine molecules inside polymer. As a result, a one-step solution casting method have been used to fabricate the biodegradable piezoelectric composite. The x-ray diffraction confirms the structure and crystalline nature of β-glycine crystals inside the chitosan matrix and hence its piezoelectric nature. The sensitivity of the fabricated pressure was about ~ 1.42 mV in the range of 5-25 kPa. The in-vitro degradability of the device has been confirmed in cell culture medium. The fabricated biodegradable sensor offers potential for point-of-care and implantable healthcare applications.