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The enrichment of Escherichia coli (E. coli) is of great significance for detection of E. coli species. In this paper, the principle of vortex velocity field generated by BAW resonator is analyzed. A hand-held device with simple operation and small size, which can quickly enrich E. coli, has been demonstrated. High efficient enrichment of E. coli in flowing samples can be achieved by using the vortex field array generated by the GHz BAW resonator array, and the whole collecting process can be completed in one minute. In addition, the experimental results show that the trapping rate of E. coli can reach 89%.

Controllable cell lysis is a crucial step for the intracellular detection, and has the potential to be applied to cell transfection. This work demonstrates a new device of acoustic pillar array chip for the parallel trapping and controllable lysis of cells. Through vibrating pillars, acoustic wave is coupled into the fluid. Radiation force and streaming field with great velocity gradient are triggered near the pillar. Cells are trapped under the radiation force and lysed by the gradient induced shear stress. Using acoustic pillar array chips, red blood cells are demonstrated to be lysed efficiently in a controllable manner.