1. Frequency of I655V SNP of HER-2/neu in colorectal cancer: a study from India
- Author
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Prahalad Singh Bharti, Vasiuddin Khan, Kapil Dev, Shahbaz Khan, Amit Kumar Verma, Rameez Hasan, Afzal Anees, and Deepti Bhatt
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medicine.medical_specialty ,education.field_of_study ,medicine.diagnostic_test ,business.industry ,Colorectal cancer ,medicine.medical_treatment ,Population ,Wild type ,Environmental Science (miscellaneous) ,medicine.disease ,Agricultural and Biological Sciences (miscellaneous) ,Gastroenterology ,Polymorphism (computer science) ,Internal medicine ,Biopsy ,Medicine ,SNP ,business ,education ,Allele frequency ,Biotechnology ,Colectomy - Abstract
The present study was conducted to determine the prognostic significance of I655V SNP (rs1136201) is a genetic one in HER-2 oncoprotein in cases of colorectal cancer (CRC). We conducted a case–control study analysing 83 subjects (naive primary CRC cases) who underwent CRC biopsy/colectomy and included 57 healthy control subjects. Analysis of HER-2 polymorphism was done by PCR-RFLP technique. The mean age was found to be 55.9 years; median age was 56 years and mode age was 54 years with a range of 43 (30–73). Males constitute 63 (75.9%) and females constitute 20 (24.1%) of patient population. According to gradewise distribution, 12 (14.45%) patients were of Grade I, 53 (63.85%) of Grade II, and 18 (21.68%) were of Grade III. We found out that out of 83 patients, 52 (62.65%) were of homozygous wild type (A/A; Ile/Ile); 27 (32.53%) were of heterozygous type (A/G; Ile/Val) and 4 (4.81%) were of homozygous mutant type (G/G; Val/Val). Allelic frequency of Ile (A) was found out to be 0.79 and that of Val (G) is 0.21 and were not significantly different from the healthy control population. Fischer’s exact p value obtained was 0.86.
- Published
- 2019
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