Search

Planococcusis a genus of Gram-positive bacteria known for potential industrial and agricultural applications. Here, we report the first draft genome sequence and phylogenomic analysis of a CRISPR-carrying, multidrug-resistant, novel candidate Planococcussp. NCCP-2050Tisolated from agricultural soil in Pakistan. The strain NCCP-2050Texhibited significant resistance to various classes of antibiotics, including fluoroquinolones (i.e., ciprofloxacin, levofloxacin, ofloxacin, moxifloxacin, and bacitracin), cephalosporins (cefotaxime, ceftazidime, cefoperazone), rifamycins (rifampicin), macrolides (erythromycin), and glycopeptides (vancomycin). Planococcussp. NCCP-2050Tconsists of genome size of 3,463,905 bp, comprised of 3639 annotated genes, including 82 carbohydrate-active enzyme genes and 39 secondary metabolite genes. The genome also contained 80 antibiotic resistance, 162 virulence, and 305 pathogen–host interaction genes along with two CRISPR arrays. Based on phylogenomic analysis, digital DNA–DNA hybridization, and average nucleotide identity values (i.e., 35.4 and 88.5%, respectively) it was suggested that strain NCCP-2050Tmight represent a potential new species within the genus Planococcus.

The present study reports the diversity of culturable bacteria associated with the puffer fish Gastrophysus spadiceus. During the study, a total of 31 strains affiliated to the genera Pseudomonas, Janthinobacterium, Rahnella,and Psychrobacterwere isolated from liver, intestines, and flesh of G. spadiceus. These strains exhibited a diverse range of metabolites as indicated by the HPLC and TLC profiles of the chemical extracts of their fermentation products. Some of these crude extracts showed strong antimicrobial activities against pathogenic bacterial strains. In addition, few crude extracts exhibit insecticidal activity against Artemia salina.

Synthesis of the exopolysaccharide-based bioflocculant Rhizobium radiobacterSZ4S7S14 was researched and medium optimized for enhanced production of bioflocculant. d-Mannose and yeast extract were found to be best carbon and nitrogen sources for maximal yield of bioflocculant. The bioflocculant samples obtained in different media with different carbon and nitrogen sources were further analyzed by SEM-EDX and FT-IR. FT-IR spectroscopy of the bioflocculant samples, obtained in different carbon/nitrogen sources showed slight modification of the structures of biopolymers. SEM micrographs revealed that exopolysaccharide-based bioflocculant of R. radiobacterSZ4S7S14 looks like bricks, and chemical structure of it can be varied due to utilized carbon and nitrogen source.