1. Inactivation of avian influenza viruses by hydrostatic pressure as a potential vaccine development approach
- Author
-
Andréa C. Oliveira, Patricia Souza dos Santos, Shana Priscila Coutinho Barroso, Alexandre Morrot, Davis Fernandes Ferreira, Jerson L. Silva, Ana Clara V. Santos, Dirlei Nico, and José Nelson Couceiro
- Subjects
Infectivity ,viral inactivation ,0303 health sciences ,biology ,030306 microbiology ,Hydrostatic pressure ,stability ,fluorescence spectroscopy ,medicine.disease_cause ,Virology ,influenza virus ,Epitope ,Influenza A virus subtype H5N1 ,Virus ,high pressure ,03 medical and health sciences ,Antigen ,Viral replication ,biology.protein ,medicine ,General Materials Science ,Antibody ,Research Articles ,030304 developmental biology - Abstract
Vaccines are a recommended strategy for controlling influenza A infections in humans and animals. Here, we describe the effects of hydrostatic pressure on the structure, morphology and functional characteristics of avian influenza A H3N8 virus. The effect of hydrostatic pressure for 3 h on H3N8 virus revealed that the particles were resistant to this condition, and the virus displayed only a discrete conformational change. We found that pressure of 3 kbar applied for 6 h was able to inhibit haemagglutination and infectivity while virus replication was no longer observed, suggesting that full virus inactivation occurred at this point. However, the neuraminidase activity was not affected at this approach suggesting the maintenance of neutralizing antibody epitopes in this key antigen. Our data bring important information for the area of structural virology of enveloped particles and support the idea of applying pressure-induced inactivation as a tool for vaccine production.
- Published
- 2021
- Full Text
- View/download PDF