1. N-Acyl Homoserine Lactone Derived Tetramic Acids Impair Photosynthesis in Phaeodactylum tricornutum
- Author
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Benjamin Bailleul, Ewout Ruysbergh, Koen Sabbe, Assaf Vardi, Sven Mangelinckx, Bernard Lepetit, Frederike Stock, Simon Backx, Peter G. Kroth, Shiri Graff van Creveld, Willem Stock, Lander Blommaert, Norbert De Kimpe, Wim Vyverman, Lachlan Dow, Anne Willems, Michail Syrpas, Universiteit Gent = Ghent University [Belgium] (UGENT), Laboratory of Protistology & Aquatic Ecology, University of Konstanz, Institut de biologie de l'ENS Paris (UMR 8197/1024) (IBENS), Département de Biologie - ENS Paris, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Faculty of Science, Department of Biochemistry and Microbiology, Laboratory of Microbiology, Gent University, Department of Biology, Institut de biologie de l'ENS Paris (IBENS), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Département de Biologie - ENS Paris, and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
0301 basic medicine ,Photosystem II ,Homoserine ,Photosynthetic efficiency ,Photosynthesis ,01 natural sciences ,Biochemistry ,03 medical and health sciences ,chemistry.chemical_compound ,4-Butyrolactone ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,Phaeodactylum tricornutum ,ComputingMilieux_MISCELLANEOUS ,Diatoms ,biology ,010405 organic chemistry ,food and beverages ,General Medicine ,biochemical phenomena, metabolism, and nutrition ,biology.organism_classification ,Pyrrolidinones ,0104 chemical sciences ,Quorum sensing ,030104 developmental biology ,N-Acyl homoserine lactone ,chemistry ,13. Climate action ,Molecular Medicine ,Bacteria - Abstract
Marine bacteria contribute substantially to nutrient cycling in the oceans and can engage in close interactions with microalgae. Many microalgae harbor characteristic satellite bacteria, many of which participate in N-acyl homoserine lactone (AHL) mediated quorum sensing. In the diffusion-controlled phycosphere, AHLs can reach high local concentrations, with some of them transforming into tetramic acids, compounds with a broad bioactivity. We tested a representative AHL, N-(3-oxododecanoyl) homoserine lactone, and its tetramic acid rearrangement product on the diatom Phaeodactylum tricornutum. While cell growth and photosynthetic efficiency of photosystem II were barely affected by the AHL, exposure to its tetramic acid rearrangement product had a negative effect on photosynthetic efficiency and led to growth inhibition and cell death in the long term, with a minimum inhibitory concentration between 20 and 50 μΜ. These results strengthen the view that AHLs may play an important role in shaping the outcome of microalgae-bacteria interactions.
- Published
- 2019