1. Immunoassay-Compatible Inactivation of SARS-CoV-2 in Plasma Samples for Enhanced Handling Safety
- Author
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Oi Wah Liew, Felic Fanusi, Jessica Yan Xia Ng, Bintou Ahmadou Ahidjo, Samantha Shi Min Ling, Shera Lilyanna, Jenny Pek Ching Chong, Angeline Eng Siew Lim, Wei Zheng Lim, Sindhu Ravindran, Justin Jang Hann Chu, Shir Lynn Lim, and Arthur Mark Richards
- Subjects
General Chemical Engineering ,General Chemistry - Abstract
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) inactivation is an important step toward enhanced biosafety in testing facilities and affords a reduction in the biocontainment level necessary for handling virus-positive biological specimens. Virus inactivation methods commonly employ heat, detergents, or combinations thereof. In this work, we address the dearth of information on the efficacy of SARS-CoV-2 inactivation procedures in plasma and their downstream impact on immunoassays. We evaluated the effects of heat (56 °C for 30 min), detergent (1-5% Triton X-100), and solvent-detergent (SD) combinations [0.3-1% tri
- Published
- 2022