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Your search keyword '"Rozeboom, Henriëtte J."' showing total 5 results
Start Over Author "Rozeboom, Henriëtte J." Journal acta crystallographica section d biological crystallography
5 results on '"Rozeboom, Henriëtte J."'

5. Crystallization and preliminary X-ray analysis of an enantioselective halohydrin dehalogenase from Agrobacterium radiobacter AD1.

Author

de Jong RM, Rozeboom HJ, Kalk KH, Tang L, Janssen DB, and Dijkstra BW

Subjects
Crystallization, Crystallography, X-Ray, Protein Conformation, Stereoisomerism, Hydrolases chemistry, Rhizobium enzymology
Abstract

Halohydrin dehalogenases are key enzymes in the bacterial degradation of vicinal halopropanols and structurally related nematocides. Crystals of the enantioselective halohydrin dehalogenase HheC from Agrobacterium radiobacter AD1 have been obtained at room temperature from hanging-drop vapour-diffusion experiments against 50-70% saturated ammonium sulfate solution at pH 6.5-7.3. The crystals belong to space group P4(1)2(1)2 or P4(3)2(1)2, with unit-cell parameters a = b = 104.5, c = 121.4 A, and contain two monomers in the asymmetric unit. The crystals diffract to 3.0 A resolution with X-rays from a Cu Kalpha rotating-anode generator.

Published
2002
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