1. Crystallization and preliminary X-ray diffraction analysis of prion protein bound to the Fab fragment of the POM1 antibody.
- Author
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Baral PK, Wieland B, Swayampakula M, Polymenidou M, Aguzzi A, Kav NN, and James MN
- Subjects
- Animals, Crystallization, Crystallography, X-Ray, Immunoglobulin Fab Fragments immunology, Mice, Prions immunology, Immunoglobulin Fab Fragments chemistry, Prions chemistry
- Abstract
Prion diseases are neurodegenerative diseases that are characterized by the conversion of the cellular prion protein PrP(c) to the pathogenic isoform PrP(sc). Several antibodies are known to interact with the cellular prion protein and to inhibit this transition. An antibody Fab fragment, Fab POM1, was produced that recognizes a structural motif of the C-terminal domain of mouse prion protein. To study the mechanism by which Fab POM1 recognizes and binds the prion molecule, the complex between Fab POM1 and the C-terminal domain of mouse prion (residues 120-232) was prepared and crystallized. Crystals of this binary complex belonged to the monoclinic space group C2, with unit-cell parameters a = 83.68, b = 106.9, c = 76.25 Å, β = 95.6°., (© 2011 International Union of Crystallography. All rights reserved.)
- Published
- 2011
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