1. Defective cyclic guanosine monophosphate-gated calcium channels and the pathogenesis of psoriasis.
- Author
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McKenzie RC, Oda Y, Szepietowski JC, Behne MJ, and Mauro T
- Subjects
- Adult, Aged, Biopsy, Needle, Calcium Signaling, Case-Control Studies, Cells, Cultured, Cyclic GMP genetics, Female, Genetic Markers genetics, Humans, Immunohistochemistry, Ion Channel Gating, Keratinocytes pathology, Male, Middle Aged, Probability, Psoriasis genetics, RNA, Messenger analysis, Reverse Transcriptase Polymerase Chain Reaction, Risk Assessment, Sampling Studies, Sensitivity and Specificity, Severity of Illness Index, Statistics, Nonparametric, Calcium Channels metabolism, Cyclic GMP metabolism, Psoriasis pathology
- Abstract
A positive association between intake of calcium channel blockers and psoriasis has been observed recently. Intake of blockers of voltage-gated calcium ion channels is associated with outbreaks of psoriasis after a latent period in patients with and without a previous family history of psoriasis. This suggests that interfering with calcium influx may trigger psoriasis. Calcium influx also occurs via cyclic guanosine monophosphate-gated channels; human keratinocytes contain functional and non-functional (splice variants) versions of these channels. We show here that keratinocytes and skin from psoriatic individuals express higher levels of mRNA encoding a non-functional cyclic guanosine monophosphate-gated calcium channel and that high expression of the splice variant by transfection of cells in culture leads to loss of protein expression for the functional cyclic guanosine monophosphate-gated Ca2+ channels.
- Published
- 2003
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