Search

The microbiota colonizing the rhizophere(immediately surrounding the root) and the endophytic microbiota(within the root) contribute to plant growth,productivity and phytoremediation.The microbiota inhibiting the rhizosphere can both benefit and undermine the plant health,whereas mutualistic andophytic microbiota may provide physiologically accessible nutrients and phytohormones to improve the plant growth,or help the plant withstand salt and drought.In this study,one phosphate-solubilizing bacterium C9 was isolated from within the root system of Caragana Korshinskii Kom.It was identificated by morphology and 16S rDNA sequencing.Using BLAST software,C9 was identified as Pantoea.The phosphate-solubilizing capacity of C9 was assayed by molydenum-antimony-D-iso-ascorbic-acid-colorimetry(MADAC) in inorganic phosphorus liquid culture.The results showed that the available phosphorus content in culture increased to 4.45mg/L and the pH value dropped to 4.22.These data indicate that the strain C9 from Caragana Korshinskii Kom.root system is able to utilize the insoluble phosphate and transform insoluble phosphorus into available form for the plants.At the same time,the possible relationship among pH level and phosphate dissolving ability was discussed.Futhermore,real-time PCR method was developed for quantifying gene copy number of C9 in roots and soil.PCR results demonstrated that the copy number of C9 follows the sequence of Caragana Korshinskii Kom.root rhizosphere soil soil around rhizosphere.Results indicate that Caragana Korshinskii Kom.exertspositive effects on the existent environment of C9 and helps improve salinity-alkalinity ecological environment gradually.