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In 29 patients with blood diseases, in 40 with other diseases and 22 healthy blood donors sucrose test for presence of plasma haemolytic factor was performed by the method of Hartmann et al. in the modification of Hansen. The percent index of haemolysis of erythrocytes after incubation with serum in 10% sucrose solution was calculated. The results were evaluated in 4 variants: 1) positive sucrose test for presence of SHF against own erythrocytes, 2) positive sucrose test for presence of SHF against foreign erythrocytes, 3) authohaemolysis in 10% sucrose and 0.9% NaCl solution, 4) haemolysis of foreign erythrocytes in 10% sucrose and 0.9% NaCl. The test was usually negative in healthy controls and patients with non-haematological diseases and in patients with leukemia it was positive in 17%.