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Inverted Follicular Keratosis (IFK) a benign tumor of skin appendages which is mostly presented as a solitary nodule with keratotic or scaling surface on the face of a middle aged or old patients but it can develop in other parts of the body. Different studies have lead to development of different theories about the role of human papillomavirus (HPV) in this kind of skin tumor. The purpose of this study is to determine the probable role of HPV in this lesion. In a cross-sectional study, we selected all the samples with the diagnosis of IFK referred to pathology department of Farabi Eye Hospital, from April 1995 to April 2006. All these samples were evaluated for histopathologic changes specific for HPV. Immunohistochemistry (IHC) staining was performed using antibody against HPV common antigen. The age range of the patients was 37 to 78 years (Mean=53). 18 patients (58%) were males and 13 patients (42%) were females. The lesion can develop in deferent areas of eyelid. Histopathologic changes regarding viral infection such as focal parakeratosis (80.6%), acanthosis (80.6%), papillomatosis (64.5%), hypergranulosis (67.7%), large keratohyaline granules (32.2%) and koilocytosis (16.1%) were evident within the lesions but HPV detection by IHC revealed positive result in any of the samples. The results about age, sex, anatomic site of the lesion and IHC staining were consistent with previous studies which consider no role for HPV in development of IFK lesions. Performing a more sensitive test such as PCR in order to strongly reject the role of HPV is recommended.

Abstract- Squamous-cell carcinoma (SCC) of the eye conjunctiva is a rare tumor. Its link with immune impairment suggests that infectious agents such as human papillomavirus (HPV) may be involved in the etiology of SCC. We conducted a case--control study on 50 SCC cases (mean age: 65.2) and 50 age frequency-matched control patients with lesion-free, normal conjunctival biopsies (mean age: 63.8) obtained from the cancer registry archive at Pathology Department of Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran, where SCC has become the most common conjunctival malignancy. MY/GP nested PCR was performed for HPV detection and E6/E7 consensus primers in combination of type specific primers were used in another nested PCR series for HPV typing. HPV DNA was detected in 46 of 50 samples of squamous cell carcinoma and none of the normal biopsies by nested PCR using primer sets of the HPV consensus L1 region (MY/GP). Subsequently, specimens from the 46 positive cases were subjected to specific PCR. Although 630bp amplicon was produced in 44 of 46 samples (E6/E7 primers), none of the specific HPV PCR reactions for HPV DNA type 16, 18, 31 or 33 resulted in the detection of HPV DNA in the 44 SCC specimens of the conjunctiva. Current results confirm the role of HPV in the etiology of conjunctival SCC. The absence of HPV 16, 18, 31 and 33 in conjunctival SCC in this study raise doubts about the role of genital types of HPV in conjunctival carcinomas. [ABSTRACT FROM AUTHOR]

Inverted Follicular Keratosis (IFK) a benign tumor of skin appendages which is mostly presented as a solitary nodule with keratotic or scaling surface on the face of a middle aged or old patients but it can develop in other parts of the body. Different studies have lead to development of different theories about the role of human papillomavirus (HPV) in this kind of skin tumor. The purpose of this study is to determine the probable role of HPV in this lesion. In a cross-sectional study, we selected all the samples with the diagnosis of IFK referred to pathology department of Farabi Eye Hospital, from April 1995 to April 2006. All these samples were evaluated for histopathologic changes specific for HPV. Immunohistochemistry (IHC) staining was performed using antibody against HPV common antigen. The age range of the patients was 37 to 78 years (Mean=53). 18 patients (58%) were males and 13 patients (42%) were females. The lesion can develop in deferent areas of eyelid. Histopathologic changes regarding viral infection such as focal parakeratosis (80.6%), acanthosis (80.6%), papillomatosis (64.5%), hypergranulosis (67.7%), large keratohyaline granules (32.2%) and koilocytosis (16.1%) were evident within the lesions but HPV detection by IHC revealed positive result in any of the samples. The results about age, sex, anatomic site of the lesion and IHC staining were consistent with previous studies which consider no role for HPV in development of IFK lesions. Performing a more sensitive test such as PCR in order to strongly reject the role of HPV is recommended. [ABSTRACT FROM AUTHOR]